HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: ARHG8 (Q7Z628)
Neuroepithelial cell-transforming gene 1 protein (Proto-oncogene p65 Net1) (Rho guanine nucleotide exchange factor 8)
Gene: NET1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.61	1.00	0.783

Property	Details
Experiments	32296183, unassigned1304
Identification Methods	two hybrid, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	RhoGEF~SH3_1, SH3_1~SOS1_NGEF_PH
Common GO terms	GO:0005829 - cytosol GO:0007165 - signal transduction
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 380 Hs			1 596
+	Hs
		594104 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		ECT2_HUMAN (70-596): 1.24e-90: 301: 17.47
+	Hs
		222181 NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00		ARHGH_HUMAN (2-592): 2e-77: 270: 18.47
+	Hs
		1453434 ITSN2_HUMAN (5-273): 6.53e-36: 141: 19.27		ITSN2_HUMAN (140-499): 2.4e-87: 299: 24.87
+	Hs
		1154182 ITSN2_HUMAN (5-273): 6.53e-36: 141: 19.27		ITSN1_HUMAN (14-499): 5.96e-80: 277: 21.77
+	Hs
		1154138 ITSN1_HUMAN (5-253): 2.96e-28: 119: 16.61		PREX1_HUMAN (127-502): 1.73e-84: 290: 20.45
+	Hs
		699860 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		ECT2_HUMAN (70-596): 1.24e-90: 301: 17.47
+	Hs
		699788 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		FGD2_HUMAN (93-587): 1.58e-88: 289: 18.47
+	Hs
		1154087 ITSN1_HUMAN (5-253): 2.96e-28: 119: 16.61		ITSN1_HUMAN (14-499): 5.96e-80: 277: 21.77
+	Hs
		699767 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		ITSN1_HUMAN (14-499): 5.96e-80: 277: 21.77
+	Hs
		584478 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		VAV_HUMAN (108-595): 1.09e-81: 275: 13.71
+	Hs
		699889 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		VAV3_HUMAN (63-588): 9.53e-82: 275: 14.37
+	Hs
		463538 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		ITSN1_HUMAN (14-499): 5.96e-80: 277: 21.77
+	Hs
		463861 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		FARP1_HUMAN (8-593): 1.54e-80: 275: 15.67
+	Hs
		473443 HCK_HUMAN (2-184): 1.58e-23: 103: 21.74		NGEF_HUMAN (4-591): 5.61e-82: 273: 16.43
+	Hs
		299586 ITSN2_HUMAN (5-273): 6.53e-36: 141: 19.27		TRIO_HUMAN (147-571): 9.68e-59: 215: 17.87
+	Hs
		463644 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		ARG28_HUMAN (80-567): 5.95e-67: 239: 18.17
+	Hs
		177361 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		ARHGB_HUMAN (156-329): 2.34e-10: 65.9: 28.18
+	Hs
		299507 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		TRIO_HUMAN (147-571): 9.68e-59: 215: 17.87
+	Hs
		177363 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		ARHGB_HUMAN (156-329): 2.34e-10: 65.9: 28.18