HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: DOK1 (Q99704)
Docking protein 1 (Downstream of tyrosine kinase 1) (p62(dok)) (pp62)
Gene: DOK1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.84	1.00	0.922

Property	Details
Experiments	26186194, 28514442, 9737977, 33961781, unassigned3799
Identification Methods	affinity chromatography technology, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	PH~SH3_1
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0007165 - signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 481
+	Mm
		237073 NCK2_MOUSE (1-380): 5.85e-94: 287: 96.05		DOK1_MOUSE (1-481): 0: 549: 83.23
+	Hs
		594088 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		DOK1_HUMAN (1-481): 0: 564: 100.00
+	Mm
		1021485 NCK1_MOUSE (1-379): 2.32e-88: 273: 68.24		DOK1_MOUSE (1-481): 0: 549: 83.23
+	Hs
		699839 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		DOK1_HUMAN (1-481): 0: 564: 100.00
+	Mm
		686951 P85A_MOUSE (166-379): 9.21e-25: 107: 16.36		DOK1_MOUSE (1-481): 0: 549: 83.23
+	Mm
		237067 NCK2_MOUSE (1-380): 5.85e-94: 287: 96.05		DOK2_MOUSE (2-426): 3.13e-118: 354: 31.31
+	Hs
		221984 NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00		DOK2_HUMAN (2-426): 3.35e-116: 349: 34.09
+	Mm
		267122 NCK1_MOUSE (1-379): 2.32e-88: 273: 68.24		DOK2_MOUSE (2-426): 3.13e-118: 354: 31.31
+	Hs
		249715 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		DOK2_HUMAN (2-426): 3.35e-116: 349: 34.09
+	Hs
		222025 NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00		IRS1_HUMAN (6-373): 1.96e-76: 262: 16.33
+	Hs
		160420 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		IRS4_HUMAN (6-404): 5.58e-95: 314: 14.35
+	Hs
		136230 P85B_HUMAN (21-380): 2.06e-23: 104: 12.80		IRS4_HUMAN (6-404): 5.58e-95: 314: 14.35
+	Rn
		772591 P85A_RAT (164-379): 1.61e-24: 107: 16.22		IRS1_RAT (6-373): 3.7e-78: 267: 17.27
+	Mm
		686938 P85A_MOUSE (166-379): 9.21e-25: 107: 16.36		IRS1_MOUSE (6-373): 1.1e-77: 265: 17.27
+	Hs
		699616 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		IRS1_HUMAN (6-373): 1.96e-76: 262: 16.33
+	Hs
		136310 P85B_HUMAN (21-380): 2.06e-23: 104: 12.80		IRS1_HUMAN (6-373): 1.96e-76: 262: 16.33
+	Mm
		686950 P85A_MOUSE (166-379): 9.21e-25: 107: 16.36		IRS2_MOUSE (6-371): 1.56e-70: 245: 15.10
+	Rn
		98921 P85A_RAT (164-379): 1.61e-24: 107: 16.22		F1MAL5_RAT (6-371): 8.48e-69: 240: 15.10
+	Hs
		699905 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		IRS2_HUMAN (6-384): 1.12e-67: 237: 14.05
+	Rn
		148123 P85A_RAT (164-379): 1.61e-24: 107: 16.22		O08724_RAT (7-315): 2.11e-60: 207: 19.09