HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: SOS1 (Q07889)
Son of sevenless homolog 1 (SOS-1)
Gene: SOS1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	1.00	0.971

Property	Details
Experiments	32203420, 8810325, 10206341, 7862111, 10026169, 33961781, 17474147, unassigned3799
Identification Methods	affinity chromatography technology, pull down, peptide array, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	RhoGEF~SH3_1, SH3_1~SOS1_NGEF_PH
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0042110 - T cell activation GO:0140693 - molecular condensate scaffold activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 1333
+	Hs
		222064 NCK2_HUMAN (1-376): 1.95e-92: 283: 68.24		SOS1_HUMAN (1-1333): 0: 1000: 100.00
+	Hs
		593975 NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00		SOS2_HUMAN (1-1131): 0: 858: 73.59
+	Hs
		222065 NCK2_HUMAN (1-376): 1.95e-92: 283: 68.24		SOS2_HUMAN (1-1131): 0: 858: 73.59
+	Hs
		1060675 GRAP_HUMAN (5-251): 9.22e-30: 115: 20.40		SOS1_HUMAN (1-1333): 0: 1000: 100.00
+	Hs
		440511 FYN_HUMAN (7-164): 5.43e-24: 105: 17.50		SOS1_HUMAN (1-1333): 0: 1000: 100.00
+	Hs
		699712 P85A_HUMAN (241-376): 5.42e-23: 102: 19.57		SOS1_HUMAN (1-1333): 0: 1000: 100.00
+	Hs
		473405 HCK_HUMAN (2-190): 3.43e-21: 96.8: 16.84		SOS1_HUMAN (1-1333): 0: 1000: 100.00
+	Hs
		440512 FYN_HUMAN (7-164): 5.43e-24: 105: 17.50		SOS2_HUMAN (1-1131): 0: 858: 73.59
+	Hs
		699713 P85A_HUMAN (241-376): 5.42e-23: 102: 19.57		SOS2_HUMAN (1-1131): 0: 858: 73.59
+	Dm
		688363 SRC42_DROME (4-127): 2.07e-18: 88.7: 23.81		SOS_DROME (8-1333): 0: 704: 41.74
+	Hs
		593989 NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00		RPGF1_HUMAN (571-1032): 2.17e-92: 324: 23.63
+	Hs
		594064 NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00		RPGF5_HUMAN (521-1015): 9.08e-83: 285: 15.03
+	Hs
		440548 FYN_HUMAN (7-164): 5.43e-24: 105: 17.50		RPGF1_HUMAN (571-1032): 2.17e-92: 324: 23.63
+	Hs
		699741 P85A_HUMAN (241-376): 5.42e-23: 102: 19.57		RPGF1_HUMAN (571-1032): 2.17e-92: 324: 23.63
+	Hs
		473415 HCK_HUMAN (2-190): 3.43e-21: 96.8: 16.84		RPGF1_HUMAN (571-1032): 2.17e-92: 324: 23.63
+	Hs
		594015 NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00		RGPS1_HUMAN (774-945): 3.83e-23: 107: 31.43
+	Hs
		699889 P85A_HUMAN (241-376): 5.42e-23: 102: 19.57		VAV3_HUMAN (163-787): 6.01e-86: 301: 15.87
+	Hs
		440335 FYN_HUMAN (7-164): 5.43e-24: 105: 17.50		VAV_HUMAN (133-667): 1.01e-80: 286: 14.15
+	Hs
		584478 P85A_HUMAN (241-376): 5.42e-23: 102: 19.57		VAV_HUMAN (133-667): 1.01e-80: 286: 14.15
+	Hs
		238796 FYN_HUMAN (7-164): 5.43e-24: 105: 17.50		KALRN_HUMAN (8-624): 5.23e-69: 259: 12.70