HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: SOS2 (Q07890)
Son of sevenless homolog 2 (SOS-2)
Gene: SOS2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.70	1.00	0.839

Property	Details
Experiments	33961781, 17474147, unassigned3799
Identification Methods	affinity chromatography technology, peptide array, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	RhoGEF~SH3_1, SH3_1~SOS1_NGEF_PH
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 1332
+	Hs
		222065 NCK2_HUMAN (1-376): 1.95e-92: 283: 68.24		SOS2_HUMAN (1-1332): 0: 947: 100.00
+	Hs
		593974 NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00		SOS1_HUMAN (1-1331): 0: 897: 67.61
+	Hs
		222064 NCK2_HUMAN (1-376): 1.95e-92: 283: 68.24		SOS1_HUMAN (1-1331): 0: 897: 67.61
+	Hs
		440512 FYN_HUMAN (7-164): 5.43e-24: 105: 17.50		SOS2_HUMAN (1-1332): 0: 947: 100.00
+	Hs
		699713 P85A_HUMAN (241-376): 5.42e-23: 102: 19.57		SOS2_HUMAN (1-1332): 0: 947: 100.00
+	Hs
		1060675 GRAP_HUMAN (5-251): 9.22e-30: 115: 20.40		SOS1_HUMAN (1-1331): 0: 897: 67.61
+	Hs
		440511 FYN_HUMAN (7-164): 5.43e-24: 105: 17.50		SOS1_HUMAN (1-1331): 0: 897: 67.61
+	Hs
		699712 P85A_HUMAN (241-376): 5.42e-23: 102: 19.57		SOS1_HUMAN (1-1331): 0: 897: 67.61
+	Hs
		473405 HCK_HUMAN (2-190): 3.43e-21: 96.8: 16.84		SOS1_HUMAN (1-1331): 0: 897: 67.61
+	Dm
		688363 SRC42_DROME (4-127): 2.07e-18: 88.7: 23.81		SOS_DROME (8-1329): 0: 697: 42.50
+	Hs
		594071 NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00		ARHG2_HUMAN (30-849): 7.3e-100: 344: 11.51
+	Hs
		593989 NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00		RPGF1_HUMAN (571-1020): 8.81e-96: 334: 25.99
+	Hs
		594064 NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00		RPGF5_HUMAN (616-1017): 1.53e-80: 278: 18.38
+	Hs
		440548 FYN_HUMAN (7-164): 5.43e-24: 105: 17.50		RPGF1_HUMAN (571-1020): 8.81e-96: 334: 25.99
+	Hs
		699741 P85A_HUMAN (241-376): 5.42e-23: 102: 19.57		RPGF1_HUMAN (571-1020): 8.81e-96: 334: 25.99
+	Hs
		473415 HCK_HUMAN (2-190): 3.43e-21: 96.8: 16.84		RPGF1_HUMAN (571-1020): 8.81e-96: 334: 25.99
+	Hs
		440335 FYN_HUMAN (7-164): 5.43e-24: 105: 17.50		VAV_HUMAN (54-665): 1.37e-86: 303: 13.04
+	Hs
		584478 P85A_HUMAN (241-376): 5.42e-23: 102: 19.57		VAV_HUMAN (54-665): 1.37e-86: 303: 13.04
+	Hs
		594015 NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00		RGPS1_HUMAN (772-936): 3.58e-19: 95.1: 30.41
+	Hs
		238796 FYN_HUMAN (7-164): 5.43e-24: 105: 17.50		KALRN_HUMAN (8-626): 8.99e-65: 245: 11.41