HitPredict

Interactor 1: R144A (P50876)
E3 ubiquitin-protein ligase RNF144A (EC 2.3.2.31) (RING finger protein 144A) (UbcM4-interacting protein 4) (Ubiquitin-conjugating enzyme 7-interacting protein 4)
Gene: RNF144A

Interactor 2: UB2L3 (P68036)
Ubiquitin-conjugating enzyme E2 L3 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme L3) (L-UBC) (UbcH7) (Ubiquitin carrier protein L3) (Ubiquitin-conjugating enzyme E2-F1) (Ubiquitin-protein ligase L3)
Gene: UBE2L3

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.89	1.00	0.947

Property	Details
Experiments	24979766, 27312108, 29311602, 32296183, 19549727, unassigned1304
Identification Methods	pull down, two hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	IBR~UQ_con
Common GO terms	GO:0000151 - ubiquitin ligase complex GO:0000209 - protein polyubiquitination GO:0005737 - cytoplasm GO:0006511 - ubiquitin-dependent protein catabolic process GO:0016567 - protein ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 292 Hs			1 154
+	Hs
		842095 R144A_HUMAN (1-292): 5.59e-97: 288: 100.00		UB2D1_HUMAN (2-149): 5.66e-75: 221: 38.26
+	Hs
		842104 R144A_HUMAN (1-292): 5.59e-97: 288: 100.00		UB2D2_HUMAN (2-149): 7.95e-75: 221: 37.58
+	Mm
		932605 R144A_MOUSE (1-292): 7.18e-97: 288: 96.58		UB2L3_MOUSE (1-154): 1.26e-64: 196: 100.00
+	Hs
		919876 RN19A_HUMAN (16-276): 5.96e-75: 246: 25.00		UB2D2_HUMAN (2-149): 7.95e-75: 221: 37.58
+	Hs
		919757 RN19B_HUMAN (13-276): 3.98e-73: 240: 25.54		UB2D2_HUMAN (2-149): 7.95e-75: 221: 37.58
+	Hs
		932539 R144B_HUMAN (3-285): 2.41e-87: 264: 54.77		UB2L3_HUMAN (1-154): 1.26e-64: 196: 100.00
+	Hs
		895819 RN19B_HUMAN (13-276): 3.98e-73: 240: 25.54		UB2D3_HUMAN (2-149): 8.29e-74: 218: 36.91
+	Hs
		159038 R144A_HUMAN (1-292): 5.59e-97: 288: 100.00		UB2L6_HUMAN (1-152): 4e-53: 166: 55.26
+	Hs
		932584 RN19A_HUMAN (16-276): 5.96e-75: 246: 25.00		UB2L3_HUMAN (1-154): 1.26e-64: 196: 100.00
+	Mm
		841456 RN19A_MOUSE (16-276): 3.28e-73: 241: 25.00		UB2L3_MOUSE (1-154): 1.26e-64: 196: 100.00
+	Hs
		932535 RN19B_HUMAN (13-276): 3.98e-73: 240: 25.54		UB2L3_HUMAN (1-154): 1.26e-64: 196: 100.00
+	Hs
		159069 R144B_HUMAN (3-285): 2.41e-87: 264: 54.77		UB2L6_HUMAN (1-152): 4e-53: 166: 55.26
+	Ce
		137091 ARI11_CAEEL (6-234): 5.04e-68: 221: 23.83		UBC18_CAEEL (1-152): 8.21e-66: 198: 59.21
+	Dm
		284374 ARI2_DROME (12-231): 1.03e-65: 215: 26.22		UBC10_DROME (1-154): 1.1e-67: 203: 74.03
+	Ce
		137086 O01963_CAEEL (6-235): 7.28e-67: 218: 22.98		UBC18_CAEEL (1-152): 8.21e-66: 198: 59.21
+	Dm
		284368 ARI2_DROME (12-231): 1.03e-65: 215: 26.22		UBC84_DROME (1-152): 7.69e-67: 201: 63.82
+	Ce
		137089 O01964_CAEEL (6-229): 6.4e-66: 215: 21.74		UBC18_CAEEL (1-152): 8.21e-66: 198: 59.21
+	Hs
		159096 RN19A_HUMAN (16-276): 5.96e-75: 246: 25.00		UB2L6_HUMAN (1-152): 4e-53: 166: 55.26
+	Hs
		159068 RN19B_HUMAN (13-276): 3.98e-73: 240: 25.54		UB2L6_HUMAN (1-152): 4e-53: 166: 55.26
+	Hs
		1190002 R144B_HUMAN (3-285): 2.41e-87: 264: 54.77		UBE2T_HUMAN (43-151): 2.92e-25: 97.1: 40.18