HitPredict

Interactor 1: UBC12 (P61081)
NEDD8-conjugating enzyme Ubc12 (EC 2.3.2.34) (NEDD8 carrier protein) (Ubiquitin-conjugating enzyme E2 M)
Gene: UBE2M

Interactor 2: UBA3 (Q8TBC4)
NEDD8-activating enzyme E1 catalytic subunit (EC 6.2.1.64) (NEDD8-activating enzyme E1C) (Ubiquitin-activating enzyme E1C) (Ubiquitin-like modifier-activating enzyme 3) (Ubiquitin-activating enzyme 3)
Gene: UBA3

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score Annotation score Interaction score
0.97 1.00 0.989

The method score is based on:

Property Details
Experiments 19250909, 10207026, 27348078, 18652489, 29973603, 30097011, 29547696, 27182664, 32145688, 35544948, 37651876, 15361859, 28514442, 17353931, 28581483, 28475037, 33961781, 35271311, 15694336, 19549727
Identification Methods enzymatic study, pull down, affinity chromatography technology, x-ray crystallography, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, two hybrid
Interaction Type direct interaction, physical association, association

The annotation score is based on:

Property Details
Interacting Pfam domains E2_bind~UQ_con, ThiF~UQ_con
Common GO terms GO:0005524 - ATP binding
GO:0005634 - nucleus
GO:0005829 - cytosol
GO:0019788 - NEDD8 transferase activity
GO:0036211 - protein modification process
and 2 others

more...
Homologous interactions 20

Homologous interactions (Top 20)

Color coding for hit scores

1 183
Hs

1 463
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Last updated: 11Aug2025