HitPredict

Interactor 1: GNB5 (O14775)
Guanine nucleotide-binding protein subunit beta-5 (Gbeta5) (Transducin beta chain 5)
Gene: GNB5

Interactor 2: GBG2 (P59768)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (G gamma-I)
Gene: GNG2

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.84	1.00	0.920

Property	Details
Experiments	33961781, 10339615, 16301321, 19376773, 12606627
Identification Methods	affinity chromatography technology, pull down, fluorescent resonance energy transfer, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	G-gamma~WD40
Common GO terms	GO:0005834 - heterotrimeric G-protein complex GO:0007186 - G protein-coupled receptor signaling pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 395 Hs			1 71
+	Hs
		154666 GNB5_HUMAN (1-395): 3.65e-115: 342: 100.00		GBG4_HUMAN (1-71): 6.54e-28: 97: 77.47
+	Mm
		837708 GNB5_MOUSE (1-395): 6.78e-115: 342: 99.24		GBG4_MOUSE (1-71): 2.19e-27: 95.5: 76.06
+	Hs
		154672 GNB5_HUMAN (1-395): 3.65e-115: 342: 100.00		GBG3_HUMAN (4-71): 3.14e-27: 95.1: 79.41
+	Hs
		154700 GNB5_HUMAN (1-395): 3.65e-115: 342: 100.00		GBG8_HUMAN (3-71): 8.51e-26: 91.7: 71.01
+	Hs
		154646 GNB5_HUMAN (1-395): 3.65e-115: 342: 100.00		GBG7_HUMAN (4-71): 4.42e-25: 89.7: 70.59
+	Hs
		154673 GNB5_HUMAN (1-395): 3.65e-115: 342: 100.00		GBG5_HUMAN (4-71): 4.11e-21: 79.7: 48.53
+	Hs
		154698 GNB5_HUMAN (1-395): 3.65e-115: 342: 100.00		GBG13_HUMAN (9-71): 3.88e-19: 74.7: 34.92
+	Dm
		669788 GBB2_DROME (51-394): 1.66e-106: 319: 35.17		GBGE_DROME (11-71): 3.98e-17: 69.7: 31.15
+	Hs
		560868 GBB3_HUMAN (13-309): 4.32e-60: 199: 16.89		GBG2_HUMAN (1-71): 3.57e-29: 100: 100.00
+	Hs
		560864 GBB3_HUMAN (13-309): 4.32e-60: 199: 16.89		GBG4_HUMAN (1-71): 6.54e-28: 97: 77.47
+	Hs
		560872 GBB3_HUMAN (13-309): 4.32e-60: 199: 16.89		GBG3_HUMAN (4-71): 3.14e-27: 95.1: 79.41
+	Hs
		560895 GBB3_HUMAN (13-309): 4.32e-60: 199: 16.89		GBG8_HUMAN (3-71): 8.51e-26: 91.7: 71.01
+	Hs
		229235 GBB3_HUMAN (13-309): 4.32e-60: 199: 16.89		GBG7_HUMAN (4-71): 4.42e-25: 89.7: 70.59
+	Hs
		560893 GBB3_HUMAN (13-309): 4.32e-60: 199: 16.89		GBG12_HUMAN (3-71): 4.46e-24: 87: 62.32
+	Hs
		560865 GBB3_HUMAN (13-309): 4.32e-60: 199: 16.89		GBG10_HUMAN (4-71): 4.34e-22: 82: 52.94
+	Hs
		560873 GBB3_HUMAN (13-309): 4.32e-60: 199: 16.89		GBG5_HUMAN (4-71): 4.11e-21: 79.7: 48.53
+	Hs
		560871 GBB3_HUMAN (13-309): 4.32e-60: 199: 16.89		GBG1_HUMAN (11-70): 2.93e-20: 77.8: 38.33
+	Hs
		560892 GBB3_HUMAN (13-309): 4.32e-60: 199: 16.89		GBG13_HUMAN (9-71): 3.88e-19: 74.7: 34.92
+	Hs
		150112 GBB3_HUMAN (13-309): 4.32e-60: 199: 16.89		GBGT2_HUMAN (12-70): 5.66e-17: 69.3: 44.07
+	Hs
		560869 GBB3_HUMAN (13-309): 4.32e-60: 199: 16.89		GBG11_HUMAN (11-70): 5.68e-17: 69.3: 38.33