HitPredict

Interactor 1: UBE2N (P61088)
Ubiquitin-conjugating enzyme E2 N (EC 2.3.2.23) (Bendless-like ubiquitin-conjugating enzyme) (E2 ubiquitin-conjugating enzyme N) (Ubc13) (UbcH13) (Ubiquitin carrier protein N) (Ubiquitin-protein ligase N)
Gene: UBE2N

Interactor 2: ZNRF1 (Q8ND25)
E3 ubiquitin-protein ligase ZNRF1 (EC 2.3.2.27) (Nerve injury-induced gene 283 protein) (RING-type E3 ubiquitin transferase ZNRF1) (Zinc/RING finger protein 1)
Gene: ZNRF1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.92	1.00	0.962

Property	Details
Experiments	27173435, 19690564, 19549727, 22797923, 29626159, 33961781
Identification Methods	affinity chromatography technology, two hybrid, pull down, x-ray crystallography, tandem affinity purification, two hybrid array, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_2
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0043161 - proteasome-mediated ubiquitin-dependent protein catabolic process
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 152 Hs			1 227
+	Hs
		1242076 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		849373 UB2D1_HUMAN (4-149): 3.22e-80: 235: 45.21		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		919790 UB2D2_HUMAN (4-149): 9.96e-80: 233: 46.58		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		895849 UB2D3_HUMAN (4-149): 1.46e-78: 230: 45.89		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		1242075 UB2E2_HUMAN (1-149): 1.52e-75: 225: 44.97		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		1242072 UB2E3_HUMAN (1-149): 3.17e-75: 224: 44.97		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		851849 UB2E1_HUMAN (1-149): 1.93e-73: 219: 45.64		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		1243628 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		Q8NEX1_HUMAN (46-227): 8.11e-77: 231: 50.55
+	Hs
		849374 UB2D1_HUMAN (4-149): 3.22e-80: 235: 45.21		Q8NEX1_HUMAN (46-227): 8.11e-77: 231: 50.55
+	Hs
		919791 UB2D2_HUMAN (4-149): 9.96e-80: 233: 46.58		Q8NEX1_HUMAN (46-227): 8.11e-77: 231: 50.55
+	Hs
		1245518 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72
+	Hs
		895850 UB2D3_HUMAN (4-149): 1.46e-78: 230: 45.89		Q8NEX1_HUMAN (46-227): 8.11e-77: 231: 50.55
+	Hs
		849375 UB2D1_HUMAN (4-149): 3.22e-80: 235: 45.21		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72
+	Hs
		919793 UB2D2_HUMAN (4-149): 9.96e-80: 233: 46.58		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72
+	Hs
		895853 UB2D3_HUMAN (4-149): 1.46e-78: 230: 45.89		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72
+	Hs
		1245511 UB2E2_HUMAN (1-149): 1.52e-75: 225: 44.97		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72
+	Hs
		1245509 UB2E3_HUMAN (1-149): 3.17e-75: 224: 44.97		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72
+	Hs
		896497 UBE2N_HUMAN (1-152): 1.51e-73: 218: 100.00		Q8NEX1_HUMAN (46-227): 8.11e-77: 231: 50.55
+	Hs
		851850 UB2E1_HUMAN (1-149): 1.93e-73: 219: 45.64		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72
+	Hs
		896498 UBE2N_HUMAN (1-152): 1.51e-73: 218: 100.00		ZNRF2_HUMAN (51-227): 9.84e-74: 225: 49.72