HitPredict

Interactor 1: BRK1 (Q8WUW1)
Protein BRICK1 (BRK1)
Gene: BRK1

Interactor 2: WASF3 (Q9UPY6)
Actin-binding protein WASF3 (Protein WAVE-3) (Verprolin homology domain-containing protein 3) (Wiskott-Aldrich syndrome protein family member 3) (WASP family protein member 3)
Gene: WASF3

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.65	0.60	0.628

Property	Details
Experiments	28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005856 - cytoskeleton GO:0030027 - lamellipodium GO:0031209 - SCAR complex GO:0070062 - extracellular exosome GO:2000601 - positive regulation of Arp2/3 complex-mediated actin nucleation
Homologous interactions	11

Homologous interactions

Color coding for hit scores

	1 75 Hs			1 502
+	Mm
		1206475 BRK1_MOUSE (1-75): 4.28e-36: 117: 98.67		WASF2_MOUSE (1-484): 9.89e-66: 222: 42.56
+	Hs
		1260577 BRK1_HUMAN (1-75): 1.59e-36: 119: 100.00		WASF1_HUMAN (1-500): 3.42e-59: 206: 43.57
+	Bt
		69091 A8YXX8_BOVIN (1-75): 1.28e-36: 119: 98.67		WASF1_BOVIN (1-500): 9.32e-59: 205: 43.93
+	Hs
		1260628 BRK1_HUMAN (1-75): 1.59e-36: 119: 100.00		WASF2_HUMAN (1-484): 2.01e-57: 200: 43.60
+	Dm
		114511 Q8MLQ0_DROME (2-75): 7.14e-36: 117: 72.97		M9PCP4_DROME (1-317): 1.62e-48: 178: 37.90
+	Dm
		1228534 Q8MLQ0_DROME (2-75): 7.14e-36: 117: 72.97		Q9VKM2_DROME (1-317): 3.53e-47: 174: 37.90
+	Dm
		94635 Q8MLQ0_DROME (2-75): 7.14e-36: 117: 72.97		F2FB81_DROME (1-209): 1.43e-42: 162: 54.55
+	At
		1149753 BRK1_ARATH (12-69): 7.29e-24: 87: 43.10		SCAR1_ARATH (1-271): 7.16e-51: 187: 18.49
+	At
		1278407 BRK1_ARATH (12-69): 7.29e-24: 87: 43.10		SCAR3_ARATH (1-162): 6.62e-47: 177: 21.69
+	At
		1140890 BRK1_ARATH (12-69): 7.29e-24: 87: 43.10		SCAR2_ARATH (1-194): 3.94e-37: 148: 21.36
+	Dd
		1122139 BRK1_DICDI (9-66): 2.5e-19: 75.1: 48.28		SCAR_DICDI (23-501): 1.2e-28: 119: 22.04