HitPredict

Interactor 1: RHOA (P61586)
Transforming protein RhoA (EC 3.6.5.2) (Rho cDNA clone 12) (h12)
Gene: RHOA

Interactor 2: ARHG1 (Q92888)
Rho guanine nucleotide exchange factor 1 (115 kDa guanine nucleotide exchange factor) (p115-RhoGEF) (p115RhoGEF) (Sub1.5)
Gene: ARHGEF1

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.89	1.00	0.944

Property	Details
Experiments	12748184, 28514442, 32203420, 33961781, 31871319, 34591642
Identification Methods	enzymatic study, affinity chromatography technology, fluorescent resonance energy transfer, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	PH_16~Ras, Ras~RhoGEF
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0007266 - Rho protein signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 193 Hs			1 912
+	Hs
		177373 RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00		ARHGB_HUMAN (8-884): 1.3e-129: 427: 30.19
+	Hs
		539989 RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00		BCR_HUMAN (7-849): 4.25e-102: 347: 13.38
+	Hs
		913634 RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00		VAV2_HUMAN (345-907): 3.46e-97: 326: 14.50
+	Hs
		962190 RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00		ARHGI_HUMAN (234-909): 9.91e-92: 319: 22.68
+	Hs
		962349 RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00		ARHG2_HUMAN (289-891): 7.67e-92: 314: 23.82
+	Hs
		962003 RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00		TIAM1_HUMAN (199-908): 5.68e-89: 312: 17.46
+	Sp
		1068728 RHO1_SCHPO (1-193): 3.62e-94: 274: 66.83		RGF1_SCHPO (322-755): 3.96e-75: 270: 21.13
+	Sp
		1439782 RHO5_SCHPO (4-193): 1.57e-93: 272: 65.31		RGF1_SCHPO (322-755): 3.96e-75: 270: 21.13
+	Hs
		962258 RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00		ARG28_HUMAN (243-911): 8.39e-86: 303: 19.82
+	Sp
		1068725 RHO1_SCHPO (1-193): 3.62e-94: 274: 66.83		RGF2_SCHPO (324-758): 1.17e-73: 265: 19.66
+	Hs
		961992 RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00		AKP13_HUMAN (275-908): 3.54e-85: 302: 23.10
+	Hs
		584505 RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00		VAV_HUMAN (362-774): 4.03e-79: 276: 20.70
+	Hs
		962367 RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00		ARHGQ_HUMAN (385-770): 5.75e-75: 265: 21.04
+	Hs
		318083 RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00		PKHG5_HUMAN (395-786): 1.35e-73: 263: 26.81
+	Hs
		962108 RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00		PKHG6_HUMAN (356-795): 2.23e-72: 256: 22.34
+	Hs
		299530 RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00		TRIO_HUMAN (353-911): 3.18e-54: 207: 14.06
+	Sc
		448555 RHO1_YEAST (3-188): 1.51e-97: 283: 73.12		ROM2_YEAST (387-679): 2.54e-15: 83.2: 25.49
+	Sp
		1068727 RHO1_SCHPO (1-193): 3.62e-94: 274: 66.83		RGF3_SCHPO (412-706): 9.87e-15: 80.9: 26.12
+	Mm
		1226826 RHOA_MOUSE (1-193): 2.21e-80: 238: 99.48		PKHG5_MOUSE (386-679): 4.07e-23: 108: 30.70
+	Hs
		962214 RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00		ARHG8_HUMAN (398-590): 5.93e-14: 77.8: 30.26