Protein: NGLY1
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase (PNGase) (hPNGase) (EC 3.5.1.52) (N-glycanase 1) (Peptide:N-glycanase)

Gene: NGLY1
Organism: Homo sapiens
Interactions: 85
UniProt ID: Q96IV0

Gene Ontology (top 5):
GO:0000224 - peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity
GO:0005634 - nucleus
GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0006457 - protein folding

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

931697

RD23B
UV excision repair protein RAD23 homolog B

9

8

Small-scale

0.754

High

935003

TERA
Transitional endoplasmic reticulum ATPase

6

1

Small-scale

0.739

High

931580

RD23A
UV excision repair protein RAD23 homolog A

6

7

Small-scale

0.730

High

1135604

UBX2B
UBX domain-containing protein 2B

4

1

High-throughput

0.698

High

1394415

UBQL1
Ubiquilin-1

4

4

High-throughput

0.698

High

731888

CEAM8
Cell adhesion molecule CEACAM8

2

2

High-throughput

0.694

High

1394405

TRI54
Tripartite motif-containing protein 54

4

0

High-throughput

0.668

High

251157

B4GT4
Beta-1,4-galactosyltransferase 4

3

0

High-throughput

0.638

High

543434

PSG1
Pregnancy-specific beta-1-glycoprotein 1

1

2

High-throughput

0.618

High

685307

PAX6
Paired box protein Pax-6

2

1

High-throughput

0.606

High

1142520

PDCD1
Programmed cell death protein 1

1

0

Small-scale

0.606

High

1394416

FAF1
FAS-associated factor 1

2

1

Small-scale

0.595

High

1179415

VMAC
Vimentin-type intermediate filament-associated coiled-coil protein

2

0

High-throughput

0.580

High

1394400

HTF4
Transcription factor 12

2

0

High-throughput

0.580

High

1394401

NPAS2
Neuronal PAS domain-containing protein 2

2

0

High-throughput

0.580

High

907224

SPHM
N-sulphoglucosamine sulphohydrolase

2

0

High-throughput

0.576

High

1394414

NAGPA
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

2

1

High-throughput

0.574

High

363710

EGFR
Epidermal growth factor receptor

2

0

Small-scale

0.559

High

1037735

PAX5
Paired box protein Pax-5

2

1

High-throughput

0.553

High

1394398

NGLY1
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

1

0

High-throughput

0.546

High

664075

PRTN3
Myeloblastin

1

1

High-throughput

0.536

High

1006626

SRPK2
SRSF protein kinase 2

1

0

High-throughput

0.527

High

1281056

VTCN1
V-set domain-containing T-cell activation inhibitor 1

1

0

Small-scale

0.517

High

1158739

PSG3
Pregnancy-specific beta-1-glycoprotein 3

1

2

High-throughput

0.514

High

282858

CRLF1
Cytokine receptor-like factor 1

1

0

High-throughput

0.513

High

561141

GILT
Gamma-interferon-inducible lysosomal thiol reductase

1

0

High-throughput

0.513

High

1154254

NPTX1
Neuronal pentraxin-1

1

0

High-throughput

0.513

High

508442

UBC
Polyubiquitin-C [Cleaved into: Ubiquitin]

1

3

High-throughput

0.501

High

1394417

NSF1C
NSFL1 cofactor p47

1

1

Small-scale

0.501

High

1292542

AMGO1
Amphoterin-induced protein 1

1

1

High-throughput

0.489

High

1360036

OIT3
Oncoprotein-induced transcript 3 protein

1

1

High-throughput

0.489

High

1394406

RTN4R
Reticulon-4 receptor

1

1

High-throughput

0.489

High

422887

A4
Amyloid-beta precursor protein

1

0

High-throughput

0.480

High

1294234

GOLI
E3 ubiquitin-protein ligase RNF130

1

1

High-throughput

0.479

High

1394413

UBQL2
Ubiquilin-2

1

2

High-throughput

0.479

High

129331

MEN1
Menin

1

0

High-throughput

0.458

High

567219

PPA5
Tartrate-resistant acid phosphatase type 5

1

0

High-throughput

0.458

High

927102

ATX3
Ataxin-3

1

0

High-throughput

0.458

High

1132315

GOGB1
Golgin subfamily B member 1

1

0

High-throughput

0.458

High

1214553

ACBD5
Acyl-CoA-binding domain-containing protein 5

1

0

High-throughput

0.458

High

1394409

PKHA4
Pleckstrin homology domain-containing family A member 4

1

0

High-throughput

0.458

High

1394410

FBX6
F-box only protein 6

1

0

High-throughput

0.458

High

1292399

OSTM1
Osteopetrosis-associated transmembrane protein 1

1

1

High-throughput

0.445

High

1056905

FMR1
Fragile X messenger ribonucleoprotein 1

1

0

High-throughput

0.438

High

188963

TMPS2
Transmembrane protease serine 2

1

2

High-throughput

0.437

High

1286865

TM11B
Transmembrane protease serine 11B

1

2

High-throughput

0.437

High

1394411

TMPS4
Transmembrane protease serine 4

1

2

High-throughput

0.437

High

350730

NOE2
Noelin-2

1

0

High-throughput

0.426

High

613434

VINC
Vinculin

1

0

High-throughput

0.426

High

1168746

DOXA2
Dual oxidase maturation factor 2

1

0

High-throughput

0.426

High

1248829

ENPP6
Glycerophosphocholine cholinephosphodiesterase ENPP6

1

0

High-throughput

0.426

High

1333763

TM87A
Transmembrane protein 87A

1

0

High-throughput

0.426

High

1369042

GGH
Gamma-glutamyl hydrolase

1

0

High-throughput

0.426

High

1224948

S22AA
Solute carrier family 22 member 10

1

1

High-throughput

0.407

High

1394418

S22AE
Solute carrier family 22 member 14

1

1

High-throughput

0.407

High

156159

TRAD1
TRAF-type zinc finger domain-containing protein 1

4

0

High-throughput

0.364

High

1394399

GUCD1
Protein GUCD1

4

0

High-throughput

0.364

High

1295022

TMM25
Transmembrane protein 25

4

0

High-throughput

0.360

High

1390493

LIGO1
Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1

3

0

High-throughput

0.345

High

1230864

BICRL
BRD4-interacting chromatin-remodeling complex-associated protein-like

2

0

High-throughput

0.316

High

346773

CER1
Cerberus

2

0

High-throughput

0.313

High

549859

CD79A
B-cell antigen receptor complex-associated protein alpha chain

2

0

High-throughput

0.313

High

1194702

GXLT1
Glucoside xylosyltransferase 1

2

0

High-throughput

0.313

High

1390860

ASIC4
Acid-sensing ion channel 4

2

0

High-throughput

0.313

High

1394402

DERL1
Derlin-1

1

0

Small-scale

0.306

High

1163799

PTPRO
Receptor-type tyrosine-protein phosphatase O

2

0

High-throughput

0.305

High

231336

EFNA2
Ephrin-A2

1

0

High-throughput

0.279

Low

450203

LIPF
Gastric triacylglycerol lipase

1

0

High-throughput

0.279

Low

597006

TSHR
Thyrotropin receptor

1

0

High-throughput

0.279

Low

1132166

ICAM4
Intercellular adhesion molecule 4

1

0

High-throughput

0.279

Low

1394403

BOC
Brother of CDO

1

0

High-throughput

0.279

Low

1394408

CST9L
Cystatin-9-like

1

0

High-throughput

0.279

Low

1394419

B3GN3
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3

1

0

High-throughput

0.279

Low

218628

TGON2
Trans-Golgi network integral membrane protein 2

1

0

High-throughput

0.249

Low

714735

KAIN
Kallistatin

1

0

High-throughput

0.232

Low

854573

S15A1
Solute carrier family 15 member 1

1

0

High-throughput

0.232

Low

1060386

SLC31
Amino acid transporter heavy chain SLC3A1

1

0

High-throughput

0.232

Low

1206272

NHLC3
NHL repeat-containing protein 3

1

0

High-throughput

0.232

Low

1231135

SCAR3
Scavenger receptor class A member 3

1

0

High-throughput

0.232

Low

1391483

BSCL2
Seipin

1

0

High-throughput

0.232

Low

1394404

S4A5
Electrogenic sodium bicarbonate cotransporter 4

1

0

High-throughput

0.232

Low

1394407

CAD23
Cadherin-23

1

0

High-throughput

0.232

Low

1394412

NPCL1
NPC1-like intracellular cholesterol transporter 1

1

0

High-throughput

0.232

Low

1394420

NTNG1
Netrin-G1

1

0

High-throughput

0.232

Low


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Last updated: 11Aug2025