Protein: NOT5
General negative regulator of transcription subunit 5

Gene: NOT5
Organism: Saccharomyces cerevisiae S288C
Interactions: 76
UniProt ID: Q12514

Gene Ontology (top 5):
GO:0000289 - nuclear-transcribed mRNA poly(A) tail shortening
GO:0000290 - deadenylation-dependent decapping of nuclear-transcribed mRNA
GO:0000932 - P-body
GO:0005634 - nucleus
GO:0005737 - cytoplasm

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

679016

NOT1
General negative regulator of transcription subunit 1

14

5

Small-scale

0.982

High

438172

NOT2
General negative regulator of transcription subunit 2

8

8

Small-scale

0.974

High

438348

NOT3
General negative regulator of transcription subunit 3

5

2

Small-scale

0.921

High

761147

NOT4
General negative regulator of transcription subunit 4

11

2

Small-scale

0.754

High

923751

CAF40
Protein CAF40

9

4

Small-scale

0.751

High

1085023

NOT5
General negative regulator of transcription subunit 5

2

2

Small-scale

0.727

High

726255

CCR4
CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4

5

6

Small-scale

0.721

High

809069

POP2
Poly(A) ribonuclease POP2

4

6

Small-scale

0.672

High

919294

CF130
Protein CAF130

3

0

Small-scale

0.623

High

858489

TAF1
Transcription initiation factor TFIID subunit 1

2

1

Small-scale

0.586

High

748114

SMY2
Protein SMY2

2

1

Small-scale

0.584

High

822216

NACB2
Nascent polypeptide-associated complex subunit beta-2

2

2

Small-scale

0.584

High

1043996

TAF12
SAGA complex/transcription factor TFIID complex subunit TAF12

2

1

Small-scale

0.584

High

1038042

NACB1
Nascent polypeptide-associated complex subunit beta-1

1

2

Small-scale

0.581

High

916625

TAF6
SAGA complex/transcription factor TFIID complex subunit TAF6

2

0

Small-scale

0.559

High

528114

HSP72
Heat shock protein SSA2

2

4

High-throughput

0.552

High

546886

SSB1
Ribosome-associated molecular chaperone SSB1

2

3

High-throughput

0.552

High

923162

BRE5
UBP3-associated protein BRE5

1

2

High-throughput

0.536

High

1084475

SCC1
Sister chromatid cohesion protein 1

1

1

High-throughput

0.536

High

548846

TAF13
Transcription initiation factor TFIID subunit 13

1

1

Small-scale

0.527

High

561554

TBP
TATA-box-binding protein

1

1

Small-scale

0.515

High

574762

KSS1
Mitogen-activated protein kinase KSS1

1

1

High-throughput

0.501

High

758353

HSL1
Probable serine/threonine-protein kinase HSL1

1

1

High-throughput

0.501

High

800788

RCK1
Serine/threonine-protein kinase RCK1

1

1

High-throughput

0.501

High

736612

RIM1
Single-stranded DNA-binding protein RIM1, mitochondrial

1

1

High-throughput

0.494

High

864764

BBC1
Myosin tail region-interacting protein MTI1

1

1

High-throughput

0.494

High

441401

KAPB
cAMP-dependent protein kinase type 2

1

1

Small-scale

0.481

High

632120

RPB4
DNA-directed RNA polymerase II subunit RPB4

1

1

Small-scale

0.481

High

678769

PAT1
Deadenylation-dependent mRNA-decapping factor PAT1

1

3

Small-scale

0.481

High

753164

NIP80
Protein NIP100

1

1

High-throughput

0.481

High

680191

CDC48
Cell division control protein 48

1

1

High-throughput

0.479

High

708631

TF2B
Transcription initiation factor IIB

1

1

High-throughput

0.479

High

776682

DEF1
RNA polymerase II degradation factor 1

1

1

High-throughput

0.479

High

794023

TAF5
SAGA complex/transcription factor TFIID complex subunit TAF5

1

1

Small-scale

0.479

High

824960

TM108
Protein TMA108

1

1

High-throughput

0.479

High

1042413

GCN5
Histone acetyltransferase GCN5

1

4

Small-scale

0.479

High

1044863

SCC2
Sister chromatid cohesion protein 2

1

1

High-throughput

0.479

High

1084080

TMA46
Translation machinery-associated protein 46

1

1

High-throughput

0.479

High

1085025

HRP1
Nuclear polyadenylated RNA-binding protein 4

1

2

Small-scale

0.479

High

796387

FL2D
Protein MUM2

1

0

High-throughput

0.460

High

823242

MED3
Mediator of RNA polymerase II transcription subunit 3

1

0

High-throughput

0.460

High

1053962

NKP2
Inner kinetochore subunit NKP2

1

0

High-throughput

0.460

High

402159

RPB1
DNA-directed RNA polymerase II subunit RPB1

1

0

Small-scale

0.458

High

738947

NAB2
Nuclear polyadenylated RNA-binding protein NAB2

1

0

Small-scale

0.458

High

806884

SKN7
Transcription factor SKN7

1

0

Small-scale

0.458

High

863157

RRP41
Exosome complex component SKI6

1

0

Small-scale

0.458

High

898206

SPT20
SAGA complex subunit SPT20

1

0

Small-scale

0.458

High

924006

CSL4
Exosome complex component CSL4

1

0

Small-scale

0.458

High

1036366

ADA2
SAGA complex subunit ADA2

1

0

Small-scale

0.458

High

945366

LSM3
LSM complex subunit LSM3

1

1

High-throughput

0.457

High

395040

EF1A
Elongation factor 1-alpha

1

1

High-throughput

0.445

High

525665

HSP71
Heat shock protein SSA1

1

4

High-throughput

0.445

High

592604

VATB
V-type proton ATPase subunit B

1

1

High-throughput

0.445

High

742759

HSP7F
Heat shock protein homolog SSE1

1

7

High-throughput

0.445

High

836983

MPG1
Mannose-1-phosphate guanyltransferase

1

1

High-throughput

0.445

High

919112

DAM1
DASH complex subunit DAM1

1

0

Small-scale

0.438

High

689169

RL16A
Large ribosomal subunit protein uL13A

1

1

High-throughput

0.437

High

748593

SC61A
Protein transport protein SEC61

1

1

High-throughput

0.437

High

365244

KPYK1
Pyruvate kinase 1

1

0

High-throughput

0.426

High

386930

TBB
Tubulin beta chain

1

0

High-throughput

0.426

High

486372

TBA1
Tubulin alpha-1 chain

1

0

High-throughput

0.426

High

863803

PFD1
Prefoldin subunit 1

1

0

High-throughput

0.426

High

924529

PFD4
Prefoldin subunit 4

1

0

High-throughput

0.426

High

747656

RSSA1
Small ribosomal subunit protein uS2A

1

1

High-throughput

0.407

High

1084445

YP150
Putative serine/threonine-protein kinase YPL150W

1

0

High-throughput

0.261

Low

370505

STF1
F(1)-ATPase inhibitor STF1, mitochondrial

1

0

High-throughput

0.251

Low

917783

KXD1
Biogenesis of lysosome-related organelles complex 1 subunit KXD1

1

0

High-throughput

0.251

Low

1085024

HPH1
Protein HPH1

1

0

High-throughput

0.251

Low

672848

ELO2
Fatty acid elongase 2

1

0

High-throughput

0.249

Low

689737

RS7A
Small ribosomal subunit protein eS7A

1

0

Small-scale

0.249

Low

795292

NU170
Nucleoporin NUP170

1

0

High-throughput

0.249

Low

825825

FIS1
Mitochondrial fission 1 protein

1

0

Small-scale

0.249

Low

835932

VMA21
Vacuolar ATPase assembly integral membrane protein VMA21

1

0

High-throughput

0.249

Low

865218

YJY1
Uncharacterized protein YJR011C

1

0

High-throughput

0.249

Low

1084819

JID1
J domain-containing protein 1

1

0

High-throughput

0.232

Low


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Last updated: 11Aug2025