Protein: NOT4
General negative regulator of transcription subunit 4 (EC 2.3.2.27) (Modulator of transcription 2)

Gene: NOT4, MOT2
Organism: Saccharomyces cerevisiae S288C
Interactions: 77
UniProt ID: P34909

Gene Ontology (top 5):
GO:0000209 - protein polyubiquitination
GO:0000289 - nuclear-transcribed mRNA poly(A) tail shortening
GO:0003723 - RNA binding
GO:0004842 - ubiquitin-protein transferase activity
GO:0005634 - nucleus

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

585697

UBC4
Ubiquitin-conjugating enzyme E2 4

8

11

Small-scale

0.967

High

585748

UBC5
Ubiquitin-conjugating enzyme E2-16 kDa

4

11

Small-scale

0.881

High

678945

NOT1
General negative regulator of transcription subunit 1

18

1

Small-scale

0.764

High

761129

CAF40
Protein CAF40

14

2

Small-scale

0.758

High

726204

CCR4
CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4

12

3

Small-scale

0.757

High

761147

NOT5
General negative regulator of transcription subunit 5

11

2

Small-scale

0.754

High

438285

NOT3
General negative regulator of transcription subunit 3

10

2

Small-scale

0.751

High

761110

POP2
Poly(A) ribonuclease POP2

9

3

Small-scale

0.748

High

438136

NOT2
General negative regulator of transcription subunit 2

8

3

Small-scale

0.745

High

761128

CF130
Protein CAF130

5

0

Small-scale

0.675

High

761121

RPN11
Ubiquitin carboxyl-terminal hydrolase RPN11

2

1

High-throughput

0.624

High

761111

DHH1
ATP-dependent RNA helicase DHH1

2

2

Small-scale

0.614

High

761106

NOT4
General negative regulator of transcription subunit 4

2

1

Small-scale

0.595

High

761107

DEF1
RNA polymerase II degradation factor 1

2

0

High-throughput

0.588

High

523545

HSP71
Heat shock protein SSA1

2

1

High-throughput

0.584

High

761136

NACB1
Nascent polypeptide-associated complex subunit beta-1

1

2

Small-scale

0.581

High

689366

RS7A
Small ribosomal subunit protein eS7A

2

1

Small-scale

0.575

High

392520

HSP82
ATP-dependent molecular chaperone HSP82

2

0

High-throughput

0.559

High

747789

SMY2
Protein SMY2

2

0

Small-scale

0.559

High

678646

PAT1
Deadenylation-dependent mRNA-decapping factor PAT1

1

1

High-throughput

0.541

High

760646

WHI3
Protein WHI3

1

1

High-throughput

0.541

High

761124

JSN1
Protein JSN1

1

1

High-throughput

0.541

High

761126

SSN8
RNA polymerase II holoenzyme cyclin-like subunit

1

0

Small-scale

0.537

High

757733

PIN4
RNA-binding protein PIN4

1

0

High-throughput

0.518

High

761132

NST1
Stress response protein NST1

1

0

High-throughput

0.518

High

761133

LSM3
LSM complex subunit LSM3

1

0

High-throughput

0.518

High

761135

PRS8
26S proteasome regulatory subunit 8 homolog

1

0

Small-scale

0.512

High

517974

SSBP1
Single-stranded nucleic acid-binding protein

1

1

High-throughput

0.501

High

636561

PSA7
Probable proteasome subunit alpha type-7

1

3

High-throughput

0.501

High

636649

PSA1
Proteasome subunit alpha type-1

1

2

High-throughput

0.501

High

661395

PSA3
Proteasome subunit alpha type-3

1

2

High-throughput

0.501

High

661493

PSA2
Proteasome subunit alpha type-2

1

3

High-throughput

0.501

High

735917

PSA5
Proteasome subunit alpha type-5

1

2

High-throughput

0.501

High

761114

PSA6
Proteasome subunit alpha type-6

1

2

High-throughput

0.501

High

761115

PSA4
Proteasome subunit alpha type-4

1

3

High-throughput

0.501

High

761125

JHD2
Histone demethylase JHD2

1

2

Small-scale

0.501

High

401958

RPB1
DNA-directed RNA polymerase II subunit RPB1

1

0

Small-scale

0.480

High

454568

TFS2
Transcription elongation factor S-II

1

0

Small-scale

0.480

High

750616

PRS6A
26S proteasome regulatory subunit 6A

1

0

Small-scale

0.480

High

438662

SC160
Protein SCP160

1

1

High-throughput

0.479

High

531296

METK1
S-adenosylmethionine synthase 1

1

1

Small-scale

0.479

High

761116

NACB2
Nascent polypeptide-associated complex subunit beta-2

1

2

High-throughput

0.479

High

761123

MTR4
ATP-dependent RNA helicase DOB1

1

1

Small-scale

0.479

High

761137

GIS1
Transcriptional activator/repressor GIS1

1

2

High-throughput

0.479

High

761146

RRP42
Exosome complex component RRP42

1

1

Small-scale

0.479

High

761148

HRP1
Nuclear polyadenylated RNA-binding protein 4

1

2

Small-scale

0.479

High

649218

SAN1
Protein SAN1

1

0

Small-scale

0.460

High

721598

RENT1
ATP-dependent helicase NAM7

1

0

Small-scale

0.460

High

594301

RPB3
DNA-directed RNA polymerase II subunit RPB3

1

0

High-throughput

0.458

High

626492

AP1
AP-1-like transcription factor YAP1

1

0

Small-scale

0.458

High

688994

RL16A
Large ribosomal subunit protein uL13A

1

0

High-throughput

0.458

High

706716

TF2B
Transcription initiation factor IIB

1

0

High-throughput

0.458

High

761109

ECM29
Proteasome component ECM29

1

0

High-throughput

0.458

High

761112

RSP5
E3 ubiquitin-protein ligase RSP5

1

0

Small-scale

0.458

High

761118

TM108
Protein TMA108

1

0

High-throughput

0.458

High

761120

DSN1
Kinetochore-associated protein DSN1

1

0

High-throughput

0.458

High

761122

RRP41
Exosome complex component SKI6

1

0

Small-scale

0.458

High

761130

CA120
CCR4-NOT transcriptional complex subunit CAF120

1

0

Small-scale

0.458

High

761131

IGO1
mRNA stability protein IGO1

1

0

Small-scale

0.458

High

761134

H3
Histone H3

1

0

High-throughput

0.458

High

761138

SCC2
Sister chromatid cohesion protein 2

1

0

High-throughput

0.458

High

761139

MUS81
Crossover junction endonuclease MUS81

1

0

High-throughput

0.458

High

761140

HBT1
Protein HBT1

1

0

High-throughput

0.458

High

761143

TMA46
Translation machinery-associated protein 46

1

0

High-throughput

0.458

High

761144

SCC1
Sister chromatid cohesion protein 1

1

0

High-throughput

0.458

High

761145

RIO1
Serine/threonine-protein kinase RIO1

1

0

High-throughput

0.458

High

761127

RS7B
Small ribosomal subunit protein eS7B

1

1

Small-scale

0.457

High

761141

WHI4
Protein WHI4

1

1

High-throughput

0.457

High

526342

HSP72
Heat shock protein SSA2

1

1

High-throughput

0.445

High

544382

SSB1
Ribosome-associated molecular chaperone SSB1

1

1

High-throughput

0.445

High

761113

SSB2
Ribosome-associated molecular chaperone SSB2

1

1

High-throughput

0.445

High

761119

MET18
DNA repair/transcription protein MET18/MMS19

1

0

High-throughput

0.438

High

621358

METK2
S-adenosylmethionine synthase 2

1

1

Small-scale

0.437

High

761142

RPN8
26S proteasome regulatory subunit RPN8

1

0

High-throughput

0.261

Low

761117

MLP2
Protein MLP2

1

0

Small-scale

0.249

Low

761108

ATG12
Ubiquitin-like protein ATG12

1

0

High-throughput

0.238

Low


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Last updated: 11Aug2025