Protein: NOT3
General negative regulator of transcription subunit 3

Gene: NOT3
Organism: Saccharomyces cerevisiae S288C
Interactions: 127
UniProt ID: P06102

Gene Ontology (top 5):
GO:0000289 - nuclear-transcribed mRNA poly(A) tail shortening
GO:0000290 - deadenylation-dependent decapping of nuclear-transcribed mRNA
GO:0000932 - P-body
GO:0005634 - nucleus
GO:0005737 - cytoplasm

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

438260

NOT1
General negative regulator of transcription subunit 1

11

5

Small-scale

0.972

High

438116

NOT2
General negative regulator of transcription subunit 2

6

8

Small-scale

0.943

High

438348

NOT5
General negative regulator of transcription subunit 5

5

2

Small-scale

0.921

High

438285

NOT4
General negative regulator of transcription subunit 4

10

2

Small-scale

0.751

High

438324

CAF40
Protein CAF40

7

4

Small-scale

0.747

High

438298

POP2
Poly(A) ribonuclease POP2

4

6

Small-scale

0.693

High

438267

CCR4
CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4

3

6

Small-scale

0.658

High

438338

WHI4
Protein WHI4

2

1

High-throughput

0.608

High

438344

YRA1
RNA annealing protein YRA1

2

1

High-throughput

0.588

High

438313

TAF1
Transcription initiation factor TFIID subunit 1

2

1

Small-scale

0.586

High

438231

PDC1
Pyruvate decarboxylase isozyme 1

2

0

High-throughput

0.563

High

438241

METK1
S-adenosylmethionine synthase 1

2

0

High-throughput

0.563

High

438347

AHA1
Hsp90 co-chaperone AHA1

2

0

High-throughput

0.563

High

438308

DSN1
Kinetochore-associated protein DSN1

2

0

High-throughput

0.559

High

438239

HSP71
Heat shock protein SSA1

2

4

High-throughput

0.552

High

438240

HSP72
Heat shock protein SSA2

2

4

High-throughput

0.552

High

438243

SSB1
Ribosome-associated molecular chaperone SSB1

2

3

High-throughput

0.552

High

438284

WHI3
Protein WHI3

1

1

High-throughput

0.541

High

438327

NST1
Stress response protein NST1

1

1

High-throughput

0.541

High

438262

RL16A
Large ribosomal subunit protein uL13A

2

1

High-throughput

0.521

High

438292

LSM2
LSM complex subunit LSM2

1

0

High-throughput

0.518

High

438317

CYP7
Peptidyl-prolyl cis-trans isomerase CYP7

1

1

High-throughput

0.515

High

438329

NACB1
Nascent polypeptide-associated complex subunit beta-1

1

2

Small-scale

0.508

High

438246

KSS1
Mitogen-activated protein kinase KSS1

1

1

High-throughput

0.501

High

438283

HSL1
Probable serine/threonine-protein kinase HSL1

1

1

High-throughput

0.501

High

438271

RIM1
Single-stranded DNA-binding protein RIM1, mitochondrial

1

1

High-throughput

0.494

High

438305

LSM5
LSM complex subunit LSM5

1

0

High-throughput

0.486

High

438232

KAPB
cAMP-dependent protein kinase type 2

1

1

Small-scale

0.481

High

438244

TAF13
Transcription initiation factor TFIID subunit 13

1

1

Small-scale

0.481

High

438248

TUP1
General transcriptional corepressor TUP1

1

1

High-throughput

0.481

High

438251

RPB4
DNA-directed RNA polymerase II subunit RPB4

1

1

Small-scale

0.481

High

438253

KIN3
Serine/threonine-protein kinase KIN3

1

2

High-throughput

0.481

High

438259

PAT1
Deadenylation-dependent mRNA-decapping factor PAT1

1

3

Small-scale

0.481

High

438280

NIP80
Protein NIP100

1

1

High-throughput

0.481

High

438297

KOG1
Target of rapamycin complex 1 subunit KOG1

1

1

High-throughput

0.481

High

438309

MAD1
Spindle assembly checkpoint component MAD1

1

1

High-throughput

0.481

High

438325

CNM67
Chaotic nuclear migration protein 67

1

1

High-throughput

0.481

High

438255

MEK1
Meiosis-specific serine/threonine-protein kinase MEK1

1

0

High-throughput

0.480

High

436258

RS31
Ubiquitin-ribosomal protein eS31 fusion protein [Cleaved into: Ubiquitin; Small ribosomal subunit protein eS31

1

2

High-throughput

0.479

High

438236

UBI4P
Polyubiquitin [Cleaved into: Ubiquitin]

1

2

High-throughput

0.479

High

438237

RL40A
Ubiquitin-ribosomal protein eL40A fusion protein [Cleaved into: Ubiquitin; Large ribosomal subunit protein eL40A

1

2

High-throughput

0.479

High

438245

TBP
TATA-box-binding protein

1

1

High-throughput

0.479

High

438263

TF2B
Transcription initiation factor IIB

1

1

High-throughput

0.479

High

438276

SMY2
Protein SMY2

1

1

High-throughput

0.479

High

438279

HSP78
Heat shock protein 78, mitochondrial

1

1

High-throughput

0.479

High

438286

DEF1
RNA polymerase II degradation factor 1

1

1

High-throughput

0.479

High

438294

RCK2
Serine/threonine-protein kinase RCK2

1

1

High-throughput

0.479

High

438307

TM108
Protein TMA108

1

1

High-throughput

0.479

High

438330

SCC2
Sister chromatid cohesion protein 2

1

1

High-throughput

0.479

High

438342

TMA46
Translation machinery-associated protein 46

1

1

High-throughput

0.479

High

438343

SCC1
Sister chromatid cohesion protein 1

1

1

High-throughput

0.479

High

438250

MYO2
Myosin-2

1

0

High-throughput

0.460

High

438266

ZIP1
Synaptonemal complex protein ZIP1

1

0

High-throughput

0.460

High

438270

SMY1
Kinesin-related protein SMY1

1

0

High-throughput

0.460

High

438278

PRS6A
26S proteasome regulatory subunit 6A

1

0

High-throughput

0.460

High

438288

NTR2
Pre-mRNA-splicing factor NTR2

1

0

High-throughput

0.460

High

438289

DAD2
DASH complex subunit DAD2

1

0

High-throughput

0.460

High

438293

CENPU
Inner kinetochore subunit AME1

1

0

High-throughput

0.460

High

438302

SNF7
Vacuolar-sorting protein SNF7

1

0

High-throughput

0.460

High

438303

PFD2
Prefoldin subunit 2

1

0

High-throughput

0.460

High

438312

BUD27
Bud site selection protein 27

1

0

High-throughput

0.460

High

438314

YJ41B
Transposon Ty4-J Gag-Pol polyprotein

1

0

High-throughput

0.460

High

438320

PFD6
Prefoldin subunit 6

1

0

High-throughput

0.460

High

438321

KNL1
Outer kinetochore KNL1 complex subunit SPC105

1

0

High-throughput

0.460

High

438326

CBK1
Serine/threonine-protein kinase CBK1

1

0

Small-scale

0.460

High

438336

NDE1
Nuclear distribution protein nudE homolog 1

1

0

High-throughput

0.460

High

438337

HDA2
HDA1 complex subunit 2

1

0

High-throughput

0.460

High

438340

SLK19
Kinetochore protein SLK19

1

0

High-throughput

0.460

High

438341

YP216
Putative ISWI chromatin-remodeling complex subunit YPL216W

1

0

High-throughput

0.460

High

438264

BMH1
Protein BMH1

1

0

High-throughput

0.458

High

438265

MCM4
DNA replication licensing factor MCM4

1

0

Small-scale

0.458

High

438269

RPC3
DNA-directed RNA polymerase III subunit RPC3

1

0

High-throughput

0.458

High

438287

CENPA
Histone H3-like centromeric protein CSE4

1

0

High-throughput

0.458

High

438290

GBLP
Small ribosomal subunit protein RACK1

1

0

Small-scale

0.458

High

438316

MPS3
Spindle pole body assembly component MPS3

1

0

High-throughput

0.458

High

438331

VMS1
tRNA endonuclease VMS1

1

0

High-throughput

0.458

High

438334

MMS22
E3 ubiquitin-protein ligase substrate receptor MMS22

1

0

High-throughput

0.458

High

438335

TFC6
Transcription factor tau 91 kDa subunit

1

0

High-throughput

0.458

High

438345

RIO1
Serine/threonine-protein kinase RIO1

1

0

High-throughput

0.458

High

394094

EF1A
Elongation factor 1-alpha

1

1

High-throughput

0.445

High

427064

RLA0
Large ribosomal subunit protein uL10

1

1

High-throughput

0.445

High

438233

DED1
ATP-dependent RNA helicase DED1

1

1

High-throughput

0.445

High

438247

VATB
V-type proton ATPase subunit B

1

1

High-throughput

0.445

High

438257

GBP2
Serine/arginine

1

2

High-throughput

0.445

High

438268

HS104
Heat shock protein 104

1

1

High-throughput

0.445

High

438273

HSP7F
Heat shock protein homolog SSE1

1

7

High-throughput

0.445

High

438274

HSP79
Heat shock protein homolog SSE2

1

7

High-throughput

0.445

High

438295

SSZ1
Ribosome-associated complex subunit SSZ1

1

1

High-throughput

0.445

High

438306

SSB2
Ribosome-associated molecular chaperone SSB2

1

3

High-throughput

0.445

High

438311

MPG1
Mannose-1-phosphate guanyltransferase

1

1

High-throughput

0.445

High

438319

KEL2
Kelch repeat-containing protein 2

1

1

High-throughput

0.439

High

438328

NUM1
Nuclear migration protein NUM1

1

1

High-throughput

0.439

High

438291

ANM1
Protein arginine N-methyltransferase 1

1

0

High-throughput

0.438

High

438277

SC61A
Protein transport protein SEC61

1

1

High-throughput

0.437

High

149477

RS9A
Small ribosomal subunit protein uS4A

1

0

High-throughput

0.426

High

432439

RL7A
Large ribosomal subunit protein uL30A

1

0

High-throughput

0.426

High

433107

RL17A
Large ribosomal subunit protein uL22A

1

0

High-throughput

0.426

High

435660

RS13
Small ribosomal subunit protein uS15

1

0

High-throughput

0.426

High

438234

FAS1
Fatty acid synthase subunit beta

1

0

High-throughput

0.426

High

438235

PYR1
Multifunctional protein URA2

1

0

High-throughput

0.426

High

438242

RL4A
Large ribosomal subunit protein uL4A

1

0

High-throughput

0.426

High

438252

XRN1
5'-3' exoribonuclease 1

1

0

High-throughput

0.426

High

438256

MAS5
Mitochondrial protein import protein MAS5

1

0

High-throughput

0.426

High

438261

RL5
Large ribosomal subunit protein uL18

1

0

High-throughput

0.426

High

438310

RL10
Large ribosomal subunit protein uL16

1

0

High-throughput

0.426

High

438249

FAS2
Fatty acid synthase subunit alpha

2

0

High-throughput

0.307

High

438258

STP22
Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease

1

0

High-throughput

0.251

Low

438272

CDC3
Cell division control protein 3

1

0

High-throughput

0.251

Low

438275

YEF3
Uncharacterized protein YEL043W

1

0

High-throughput

0.251

Low

438282

TIP20
Protein transport protein TIP20

1

0

High-throughput

0.251

Low

438296

LAM4
Membrane-anchored lipid-binding protein LAM4

1

0

High-throughput

0.251

Low

438301

MBO1
Gamma tubulin complex adapter SPC72

1

0

High-throughput

0.251

Low

438304

TSC11
Target of rapamycin complex 2 subunit TSC11

1

0

High-throughput

0.251

Low

438315

YJE9
Uncharacterized protein YJL049W

1

0

High-throughput

0.251

Low

438318

NUP57
Nucleoporin NUP57

1

0

High-throughput

0.251

Low

438322

MPS2
Monopolar spindle protein 2

1

0

High-throughput

0.251

Low

438323

NNF2
Protein NNF2

1

0

High-throughput

0.251

Low

438332

GLU2B
Glucosidase 2 subunit beta

1

0

High-throughput

0.251

Low

438333

HOB2
Protein YPR117W

1

0

High-throughput

0.251

Low

438339

YL031
Putative uncharacterized protein YLR031W

1

0

High-throughput

0.251

Low

438346

RUD3
GRIP domain-containing protein RUD3

1

0

High-throughput

0.251

Low

438349

MPD2
Protein disulfide isomerase MPD2

1

0

High-throughput

0.251

Low

438281

MFT1
THO complex subunit MFT1

1

0

Small-scale

0.249

Low

438300

LAA1
AP-1 accessory protein LAA1

1

0

High-throughput

0.249

Low

438238

RS24A
Small ribosomal subunit protein eS24A

1

0

High-throughput

0.232

Low

438254

RS3A2
Small ribosomal subunit protein eS1B

1

0

High-throughput

0.232

Low

438299

RS14B
Small ribosomal subunit protein uS11B

1

0

High-throughput

0.232

Low


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Last updated: 11Aug2025