Protein: MED3
Mediator of RNA polymerase II transcription subunit 3 (Hyper-recombination suppressor protein 1) (Mediator complex subunit 3) (Poly-glutamine domain protein 1)

Gene: MED3, PGD1
Organism: Saccharomyces cerevisiae S288C
Interactions: 76
UniProt ID: P40356

Gene Ontology (top 5):
GO:0000122 - negative regulation of transcription by RNA polymerase II
GO:0000979 - RNA polymerase II core promoter sequence-specific DNA binding
GO:0003713 - transcription coactivator activity
GO:0005634 - nucleus
GO:0016592 - mediator complex

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

823234

MED2
Mediator of RNA polymerase II transcription subunit 2

9

0

Small-scale

0.916

High

740801

MED18
Mediator of RNA polymerase II transcription subunit 18

10

0

Small-scale

0.720

High

619432

MED14
Mediator of RNA polymerase II transcription subunit 14

9

0

Small-scale

0.714

High

624918

MED15
Mediator of RNA polymerase II transcription subunit 15

8

0

Small-scale

0.714

High

740413

MED17
Mediator of RNA polymerase II transcription subunit 17

8

0

Small-scale

0.712

High

668493

MED19
Mediator of RNA polymerase II transcription subunit 19

6

0

Small-scale

0.689

High

823231

MED7
Mediator of RNA polymerase II transcription subunit 7

6

0

Small-scale

0.686

High

823239

MED4
Mediator of RNA polymerase II transcription subunit 4

5

0

Small-scale

0.684

High

797709

MED8
Mediator of RNA polymerase II transcription subunit 8

6

0

Small-scale

0.683

High

740462

MED22
Mediator of RNA polymerase II transcription subunit 22

5

0

Small-scale

0.682

High

823238

MED1
Mediator of RNA polymerase II transcription subunit 1

5

0

Small-scale

0.676

High

804023

MED6
Mediator of RNA polymerase II transcription subunit 6

5

0

Small-scale

0.670

High

823219

MED21
Mediator of RNA polymerase II transcription subunit 21

4

0

Small-scale

0.658

High

823220

MED5
Mediator of RNA polymerase II transcription subunit 5

4

0

Small-scale

0.649

High

733543

MED16
Mediator of RNA polymerase II transcription subunit 16

4

0

Small-scale

0.644

High

751210

MED9
Mediator of RNA polymerase II transcription subunit 9

3

0

Small-scale

0.622

High

810648

SSN3
Meiotic mRNA stability protein kinase SSN3

2

1

Small-scale

0.618

High

598410

TUP1
General transcriptional corepressor TUP1

2

0

Small-scale

0.598

High

757586

MED20
Mediator of RNA polymerase II transcription subunit 20

3

0

Small-scale

0.597

High

801152

MED31
Mediator of RNA polymerase II transcription subunit 31

2

0

High-throughput

0.576

High

823225

MED10
Mediator of RNA polymerase II transcription subunit 10

2

0

High-throughput

0.576

High

594404

RPB3
DNA-directed RNA polymerase II subunit RPB3

2

0

Small-scale

0.559

High

577555

CYC8
General transcriptional corepressor CYC8

1

0

Small-scale

0.557

High

678811

SRB8
Mediator of RNA polymerase II transcription subunit 12

2

0

Small-scale

0.547

High

395247

GCN4
General control transcription factor GCN4

1

0

Small-scale

0.537

High

547872

NHP6B
Non-histone chromosomal protein 6B

1

1

Small-scale

0.527

High

721996

XPO1
Exportin-1

1

0

High-throughput

0.513

High

666688

GRR1
SCF E3 ubiquitin ligase complex F-box protein GRR1

1

0

Small-scale

0.504

High

823236

MSH5
MutS protein homolog 5

1

0

High-throughput

0.486

High

547798

NHP6A
Non-histone chromosomal protein 6A

1

1

Small-scale

0.481

High

802133

STB5
Protein STB5

1

1

Small-scale

0.481

High

823179

RAD26
DNA repair and recombination protein RAD26

1

1

High-throughput

0.481

High

602493

PHO85
Cyclin-dependent protein kinase PHO85

1

0

High-throughput

0.480

High

408417

GAL4
Regulatory protein GAL4

1

1

Small-scale

0.479

High

734431

BTAF1
TATA-binding protein-associated factor MOT1

1

1

Small-scale

0.479

High

646049

KIN3
Serine/threonine-protein kinase KIN3

1

0

High-throughput

0.460

High

750688

PRS6A
26S proteasome regulatory subunit 6A

1

0

High-throughput

0.460

High

777059

BDF1
Bromodomain-containing factor 1

1

0

High-throughput

0.460

High

797883

CENPU
Inner kinetochore subunit AME1

1

0

High-throughput

0.460

High

823217

NNF1
Kinetochore-associated protein NNF1

1

0

High-throughput

0.460

High

823222

CIK1
Spindle pole body-associated protein CIK1

1

0

High-throughput

0.460

High

823223

SPC24
Kinetochore protein SPC24

1

0

High-throughput

0.460

High

823224

NKP2
Inner kinetochore subunit NKP2

1

0

High-throughput

0.460

High

823226

ATG17
Autophagy-related protein 17

1

0

High-throughput

0.460

High

823227

ARR1
Arsenical-resistance protein ARR1

1

0

High-throughput

0.460

High

823228

HDA2
HDA1 complex subunit 2

1

0

High-throughput

0.460

High

823233

NOP53
Ribosome biogenesis protein NOP53

1

0

High-throughput

0.460

High

823242

NOT5
General negative regulator of transcription subunit 5

1

0

High-throughput

0.460

High

579508

HSC82
ATP-dependent molecular chaperone HSC82

1

0

High-throughput

0.458

High

707268

TF2B
Transcription initiation factor IIB

1

0

High-throughput

0.458

High

763332

TAF14
Transcription initiation factor TFIID subunit 14

1

0

High-throughput

0.458

High

776589

DEF1
RNA polymerase II degradation factor 1

1

0

High-throughput

0.458

High

807812

SSN2
Mediator of RNA polymerase II transcription subunit 13

1

0

High-throughput

0.458

High

823218

SSN8
RNA polymerase II holoenzyme cyclin-like subunit

1

0

High-throughput

0.458

High

823232

TAF10
SAGA complex/transcription factor TFIID complex subunit TAF10

1

0

High-throughput

0.458

High

823235

SCC1
Sister chromatid cohesion protein 1

1

0

High-throughput

0.458

High

823243

MED11
Mediator of RNA polymerase II transcription subunit 11

1

0

High-throughput

0.458

High

524134

HSP71
Heat shock protein SSA1

1

0

High-throughput

0.426

High

545098

SSB1
Ribosome-associated molecular chaperone SSB1

1

0

High-throughput

0.426

High

782575

DAD2
DASH complex subunit DAD2

2

0

High-throughput

0.304

High

657094

IF5A1
Eukaryotic translation initiation factor 5A-1

1

0

High-throughput

0.269

Low

823230

ALR1
Magnesium transporter ALR1

1

0

High-throughput

0.265

Low

677757

STP22
Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease

1

0

High-throughput

0.251

Low

790325

VPP2
V-type proton ATPase subunit a, Golgi isoform

1

0

High-throughput

0.251

Low

796318

FL2D
Protein MUM2

1

0

High-throughput

0.251

Low

804897

LAM4
Membrane-anchored lipid-binding protein LAM4

1

0

High-throughput

0.251

Low

808252

VPS41
Vacuolar protein sorting-associated protein 41

1

0

Small-scale

0.251

Low

813804

MBO1
Gamma tubulin complex adapter SPC72

1

0

High-throughput

0.251

Low

815019

SNF7
Vacuolar-sorting protein SNF7

1

0

High-throughput

0.251

Low

823215

BNR1
BNI1-related protein 1

1

0

High-throughput

0.251

Low

823216

FMP32
Protein FMP32, mitochondrial

1

0

High-throughput

0.251

Low

823221

HSK3
DASH complex subunit HSK3

1

0

High-throughput

0.251

Low

823229

IRC3
Putative ATP-dependent helicase IRC3

1

0

High-throughput

0.251

Low

823237

DAD1
DASH complex subunit DAD1

1

0

High-throughput

0.251

Low

823240

GYP5
GTPase-activating protein GYP5

1

0

High-throughput

0.251

Low

823241

ERP3
Protein ERP3

1

0

High-throughput

0.251

Low


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Last updated: 11Aug2025