Protein: NOT1
General negative regulator of transcription subunit 1 (Cell division cycle protein 39)

Gene: NOT1, CDC39
Organism: Saccharomyces cerevisiae S288C
Interactions: 121
UniProt ID: P25655

Gene Ontology (top 5):
GO:0000288 - nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
GO:0000289 - nuclear-transcribed mRNA poly(A) tail shortening
GO:0000749 - response to pheromone triggering conjugation with cellular fusion
GO:0000932 - P-body
GO:0001671 - ATPase activator activity

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

678958

POP2
Poly(A) ribonuclease POP2

16

8

Small-scale

0.988

High

679016

NOT5
General negative regulator of transcription subunit 5

14

5

Small-scale

0.982

High

678987

CAF40
Protein CAF40

14

3

Small-scale

0.982

High

438126

NOT2
General negative regulator of transcription subunit 2

13

8

Small-scale

0.980

High

438260

NOT3
General negative regulator of transcription subunit 3

11

5

Small-scale

0.972

High

678963

DHH1
ATP-dependent RNA helicase DHH1

4

3

Small-scale

0.888

High

678927

NOT1
General negative regulator of transcription subunit 1

2

1

High-throughput

0.811

High

678945

NOT4
General negative regulator of transcription subunit 4

18

1

Small-scale

0.764

High

678934

CCR4
CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4

16

9

Small-scale

0.764

High

678984

CF130
Protein CAF130

10

0

Small-scale

0.724

High

679015

RUVB2
RuvB-like protein 2

3

1

High-throughput

0.667

High

678969

NACB2
Nascent polypeptide-associated complex subunit beta-2

3

1

Small-scale

0.661

High

678954

LSM2
LSM complex subunit LSM2

2

1

High-throughput

0.613

High

679014

TFC7
Transcription factor tau 55 kDa subunit

2

0

High-throughput

0.576

High

678975

TAF1
Transcription initiation factor TFIID subunit 1

1

0

Small-scale

0.568

High

678940

SMY2
Protein SMY2

2

0

Small-scale

0.559

High

678951

CAF4
CCR4-associated factor 4

1

2

Small-scale

0.552

High

543994

SSB1
Ribosome-associated molecular chaperone SSB1

2

3

High-throughput

0.552

High

678948

DEF1
RNA polymerase II degradation factor 1

2

0

High-throughput

0.551

High

678972

DSN1
Kinetochore-associated protein DSN1

2

0

High-throughput

0.547

High

672090

PUF4
Pumilio homology domain family member 4

1

5

High-throughput

0.541

High

678944

WHI3
Protein WHI3

1

1

High-throughput

0.541

High

678959

MPT5
Suppressor protein MPT5

1

5

High-throughput

0.541

High

678977

LSM8
LSM2-LSM8 complex subunit LSM8

1

1

High-throughput

0.541

High

679006

WHI4
Protein WHI4

1

1

High-throughput

0.541

High

574557

YAK1
Dual specificity protein kinase YAK1

1

3

High-throughput

0.536

High

678986

BRE5
UBP3-associated protein BRE5

1

1

High-throughput

0.536

High

679001

RUVB1
RuvB-like protein 1

1

1

High-throughput

0.536

High

678956

SKN7
Transcription factor SKN7

1

0

Small-scale

0.529

High

678943

PIN4
RNA-binding protein PIN4

1

0

High-throughput

0.518

High

678967

LSM4
LSM complex subunit LSM4

1

0

High-throughput

0.518

High

678988

NST1
Stress response protein NST1

1

0

High-throughput

0.518

High

678990

LSM3
LSM complex subunit LSM3

1

0

High-throughput

0.518

High

679003

LSM6
LSM complex subunit LSM6

1

0

High-throughput

0.518

High

678994

PRS8
26S proteasome regulatory subunit 8 homolog

1

2

Small-scale

0.515

High

678952

ANM1
Protein arginine N-methyltransferase 1

1

0

High-throughput

0.513

High

678993

UBP3
Ubiquitin carboxyl-terminal hydrolase 3

1

0

High-throughput

0.513

High

679007

RBX1
RING-box protein HRT1

1

0

High-throughput

0.513

High

678998

NACB1
Nascent polypeptide-associated complex subunit beta-1

1

1

Small-scale

0.508

High

517920

SSBP1
Single-stranded nucleic acid-binding protein

1

1

High-throughput

0.501

High

678938

RPN2
26S proteasome regulatory subunit RPN2

1

0

High-throughput

0.493

High

678965

NPT1
Nicotinate phosphoribosyltransferase

1

0

High-throughput

0.493

High

678978

SSN8
RNA polymerase II holoenzyme cyclin-like subunit

1

0

Small-scale

0.493

High

678973

COPB2
Coatomer subunit beta'

1

1

High-throughput

0.489

High

678985

COPA
Coatomer subunit alpha

1

1

High-throughput

0.489

High

678960

SSN3
Meiotic mRNA stability protein kinase SSN3

1

0

Small-scale

0.486

High

678933

RENT1
ATP-dependent helicase NAM7

1

2

Small-scale

0.481

High

454558

TFS2
Transcription elongation factor S-II

1

0

Small-scale

0.480

High

678966

RSP5
E3 ubiquitin-protein ligase RSP5

1

0

Small-scale

0.480

High

380989

H2B1
Histone H2B.1

1

1

High-throughput

0.479

High

381427

H2B2
Histone H2B.2

1

1

High-throughput

0.479

High

381905

H4
Histone H4

1

1

High-throughput

0.479

High

438627

SC160
Protein SCP160

1

1

High-throughput

0.479

High

678935

HS104
Heat shock protein 104

1

1

High-throughput

0.479

High

678941

PRS7
26S proteasome regulatory subunit 7 homolog

1

2

Small-scale

0.479

High

678949

RS27A
Small ribosomal subunit protein eS27A

1

1

High-throughput

0.479

High

678950

CENPA
Histone H3-like centromeric protein CSE4

1

1

High-throughput

0.479

High

678970

PRS4
26S proteasome regulatory subunit 4 homolog

1

1

Small-scale

0.479

High

678974

SCD6
Protein SCD6

1

1

Small-scale

0.479

High

678981

UBC9
SUMO-conjugating enzyme UBC9

1

1

High-throughput

0.479

High

678982

CDH1
APC/C activator protein CDH1

1

1

Small-scale

0.479

High

678989

APJ1
J domain-containing protein APJ1

1

1

High-throughput

0.479

High

678991

H3
Histone H3

1

1

High-throughput

0.479

High

679000

TAF12
SAGA complex/transcription factor TFIID complex subunit TAF12

1

1

High-throughput

0.479

High

679002

SIZ1
E3 SUMO-protein ligase SIZ1

1

1

High-throughput

0.479

High

679013

SIZ2
E3 SUMO-protein ligase SIZ2

1

1

High-throughput

0.479

High

679017

HRP1
Nuclear polyadenylated RNA-binding protein 4

1

1

Small-scale

0.479

High

359162

G3P3
Glyceraldehyde-3-phosphate dehydrogenase 3

1

0

Small-scale

0.460

High

434871

RS3
Small ribosomal subunit protein uS3

1

0

High-throughput

0.458

High

561426

TBP
TATA-box-binding protein

1

0

Small-scale

0.458

High

585683

UBC4
Ubiquitin-conjugating enzyme E2 4

1

0

High-throughput

0.458

High

594224

RPB3
DNA-directed RNA polymerase II subunit RPB3

1

0

High-throughput

0.458

High

664251

SSD1
Protein SSD1

1

0

Small-scale

0.458

High

678928

CDC48
Cell division control protein 48

1

0

High-throughput

0.458

High

678929

RL16A
Large ribosomal subunit protein uL13A

1

0

High-throughput

0.458

High

678931

TF2B
Transcription initiation factor IIB

1

0

High-throughput

0.458

High

678932

MCM4
DNA replication licensing factor MCM4

1

0

Small-scale

0.458

High

678936

RPC3
DNA-directed RNA polymerase III subunit RPC3

1

0

High-throughput

0.458

High

678946

SPT10
Protein SPT10

1

0

High-throughput

0.458

High

678947

SPT21
Protein SPT21

1

0

High-throughput

0.458

High

678953

ATG8
Autophagy-related protein 8

1

0

High-throughput

0.458

High

678971

TM108
Protein TMA108

1

0

High-throughput

0.458

High

678980

RS12
Small ribosomal subunit protein eS12

1

0

High-throughput

0.458

High

679008

SGO1
Shugoshin

1

0

High-throughput

0.458

High

679010

TMA46
Translation machinery-associated protein 46

1

0

High-throughput

0.458

High

679012

RIO1
Serine/threonine-protein kinase RIO1

1

0

High-throughput

0.458

High

481508

HSP73
Heat shock protein SSA3

1

2

High-throughput

0.445

High

526051

HSP72
Heat shock protein SSA2

1

2

High-throughput

0.445

High

670394

SIS1
Protein SIS1

1

1

High-throughput

0.445

High

675752

MAS5
Mitochondrial protein import protein MAS5

1

1

High-throughput

0.445

High

678939

HSP7F
Heat shock protein homolog SSE1

1

3

High-throughput

0.445

High

678942

HSP78
Heat shock protein 78, mitochondrial

1

1

High-throughput

0.445

High

678955

SSZ1
Ribosome-associated complex subunit SSZ1

1

2

High-throughput

0.445

High

678962

CAJ1
Protein CAJ1

1

1

High-throughput

0.445

High

678968

SSB2
Ribosome-associated molecular chaperone SSB2

1

3

High-throughput

0.445

High

679004

SP382
Pre-mRNA-splicing factor SPP382

1

0

High-throughput

0.438

High

678930

RS7A
Small ribosomal subunit protein eS7A

1

1

High-throughput

0.437

High

392320

HSP82
ATP-dependent molecular chaperone HSP82

1

0

High-throughput

0.426

High

394288

EF1A
Elongation factor 1-alpha

1

0

High-throughput

0.426

High

453448

PYR1
Multifunctional protein URA2

1

0

High-throughput

0.426

High

592050

VATB
V-type proton ATPase subunit B

1

0

High-throughput

0.426

High

653826

HSP12
12 kDa heat shock protein

1

0

High-throughput

0.426

High

678964

YM11
Uncharacterized protein YMR124W

1

0

High-throughput

0.426

High

678999

NSE5
Non-structural maintenance of chromosome element 5

1

0

High-throughput

0.426

High

678995

UBC7
Ubiquitin-conjugating enzyme E2 7

1

1

High-throughput

0.417

High

679005

SWA2
Auxilin-like clathrin uncoating factor SWA2

1

1

High-throughput

0.407

High

678976

YJY1
Uncharacterized protein YJR011C

2

0

Small-scale

0.323

High

678957

SSN2
Mediator of RNA polymerase II transcription subunit 13

1

0

Small-scale

0.288

High

678937

RIM1
Single-stranded DNA-binding protein RIM1, mitochondrial

1

0

High-throughput

0.282

High

679011

ATG9
Autophagy-related protein 9

1

0

High-throughput

0.279

Low

678979

ADRO
Probable NADPH:adrenodoxin oxidoreductase, mitochondrial

1

0

High-throughput

0.265

Low

438391

EIF3B
Eukaryotic translation initiation factor 3 subunit B

1

0

Small-scale

0.249

Low

441740

RS14A
Small ribosomal subunit protein uS11A

1

0

High-throughput

0.249

Low

512854

RS8A
Small ribosomal subunit protein eS8A

1

0

High-throughput

0.249

Low

678992

NUM1
Nuclear migration protein NUM1

1

0

High-throughput

0.249

Low

678996

MLP1
Protein MLP1

1

0

Small-scale

0.249

Low

678997

NU100
Nucleoporin NUP100/NSP100

1

0

Small-scale

0.249

Low

679009

RS10A
Small ribosomal subunit protein eS10A

1

0

High-throughput

0.249

Low

678961

TCPD
T-complex protein 1 subunit delta

1

0

High-throughput

0.232

Low

678983

TAM41
Phosphatidate cytidylyltransferase, mitochondrial

1

0

High-throughput

0.232

Low


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Last updated: 11Aug2025