Protein: SPOT
Bifunctional (p)ppGpp synthase/hydrolase SpoT [Includes: GTP pyrophosphokinase (EC 2.7.6.5) ((p)ppGpp synthase) (ATP:GTP 3'-pyrophosphotransferase) (Stringent response-like protein) (ppGpp synthase II); Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) (Penta-phosphate guanosine-3'-pyrophosphohydrolase) ((ppGpp)ase)]

Gene: SPOT
Organism: Escherichia coli K-12
Interactions: 67
UniProt ID: P0AG24

Gene Ontology (top 5):
GO:0005829 - cytosol
GO:0008728 - GTP diphosphokinase activity
GO:0008893 - guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity
GO:0015949 - nucleobase-containing small molecule interconversion
GO:0015969 - guanosine tetraphosphate metabolic process

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

489306

ACP
Acyl carrier protein

3

2

Small-scale

0.631

High

501091

SPOT
Bifunctional

1

8

Small-scale

0.590

High

492610

RL19
Large ribosomal subunit protein bL19

2

1

High-throughput

0.588

High

497621

RLUC
Ribosomal large subunit pseudouridine synthase C

2

1

High-throughput

0.588

High

501106

RL2
Large ribosomal subunit protein uL2

2

1

High-throughput

0.588

High

490885

HFQ
RNA-binding protein Hfq

2

0

High-throughput

0.563

High

492506

RL7
Large ribosomal subunit protein bL12

2

0

High-throughput

0.563

High

493159

RL9
Large ribosomal subunit protein bL9

2

0

High-throughput

0.563

High

493247

RS10
Small ribosomal subunit protein uS10

2

0

High-throughput

0.563

High

493918

RS3
Small ribosomal subunit protein uS3

2

0

High-throughput

0.563

High

494095

RS4
Small ribosomal subunit protein uS4

2

0

High-throughput

0.563

High

494377

RS8
Small ribosomal subunit protein uS8

2

0

High-throughput

0.563

High

494447

RS9
Small ribosomal subunit protein uS9

2

0

High-throughput

0.563

High

497496

RL13
Large ribosomal subunit protein uL13

2

0

High-throughput

0.563

High

499059

DBHA
DNA-binding protein HU-alpha

2

0

High-throughput

0.563

High

501093

RL17
Large ribosomal subunit protein bL17

2

0

High-throughput

0.563

High

501094

RL21
Large ribosomal subunit protein bL21

2

0

High-throughput

0.563

High

501098

RL18
Large ribosomal subunit protein uL18

2

0

High-throughput

0.563

High

501103

RLMN
Dual-specificity RNA methyltransferase RlmN

2

0

High-throughput

0.563

High

501108

RL24
Large ribosomal subunit protein uL24

2

0

High-throughput

0.563

High

492113

NADK
NAD kinase

2

0

High-throughput

0.547

High

501111

FLAV
Flavodoxin 1

1

0

High-throughput

0.513

High

495823

RPOC
DNA-directed RNA polymerase subunit beta'

1

1

High-throughput

0.484

High

498373

CLPA
ATP-dependent Clp protease ATP-binding subunit ClpA

1

1

High-throughput

0.484

High

501100

SRMB
ATP-dependent RNA helicase SrmB

1

2

High-throughput

0.484

High

501104

RLUB
Ribosomal large subunit pseudouridine synthase B

1

1

High-throughput

0.484

High

501109

RL4
Large ribosomal subunit protein uL4

1

1

High-throughput

0.484

High

495483

RHLB
ATP-dependent RNA helicase RhlB

1

2

High-throughput

0.479

High

382576

RS6
Small ribosomal subunit protein bS6

1

0

High-throughput

0.464

High

382696

RS7
Small ribosomal subunit protein uS7

1

0

High-throughput

0.464

High

492937

RL28
Large ribosomal subunit protein bL28

1

0

High-throughput

0.464

High

493309

RS11
Small ribosomal subunit protein uS11

1

0

High-throughput

0.464

High

493334

RS12
Small ribosomal subunit protein uS12

1

0

High-throughput

0.464

High

493538

RS18
Small ribosomal subunit protein bS18

1

0

High-throughput

0.464

High

493629

RS20
Small ribosomal subunit protein bS20

1

0

High-throughput

0.464

High

494284

RS5
Small ribosomal subunit protein uS5

1

0

High-throughput

0.464

High

494666

RPOZ
DNA-directed RNA polymerase subunit omega

1

0

High-throughput

0.464

High

495398

YIHI
Der GTPase-activating protein YihI

1

0

High-throughput

0.464

High

497093

DEAD
ATP-dependent RNA helicase DeaD

1

0

High-throughput

0.464

High

500482

SKP
Chaperone protein Skp

1

0

High-throughput

0.464

High

501101

MUKB
Chromosome partition protein MukB

1

0

High-throughput

0.464

High

501107

RL3
Large ribosomal subunit protein uL3

1

0

High-throughput

0.464

High

501110

RL22
Large ribosomal subunit protein uL22

1

0

High-throughput

0.464

High

492735

RL1
Large ribosomal subunit protein uL1

1

0

High-throughput

0.458

High

495569

TMAR
Pole-localizer protein TmaR

1

0

High-throughput

0.458

High

501092

RHO
Transcription termination factor Rho

1

0

High-throughput

0.458

High

501102

PSS
CDP-diacylglycerol--serine O-phosphatidyltransferase PssA

1

0

High-throughput

0.458

High

498670

TOLR
Tol-Pal system protein TolR

1

2

High-throughput

0.442

High

384031

RL15
Large ribosomal subunit protein uL15

2

0

High-throughput

0.307

High

493486

RS16
Small ribosomal subunit protein bS16

2

0

High-throughput

0.307

High

501095

RS14
Small ribosomal subunit protein uS14

2

0

High-throughput

0.307

High

501096

RS1
Small ribosomal subunit protein bS1

2

0

High-throughput

0.307

High

501097

TRMH
tRNA

2

0

High-throughput

0.307

High

492310

RECF
DNA replication and repair protein RecF

1

0

High-throughput

0.279

Low

500994

RNK
Regulator of nucleoside diphosphate kinase

1

0

High-throughput

0.279

Low

501115

QUEC
7-cyano-7-deazaguanine synthase

1

0

High-throughput

0.279

Low

491938

RPPH
RNA pyrophosphohydrolase

1

0

High-throughput

0.253

Low

493752

RS2
Small ribosomal subunit protein uS2

1

0

High-throughput

0.253

Low

494565

RNPA
Ribonuclease P protein component

1

0

High-throughput

0.253

Low

501099

EFTU1
Elongation factor Tu 1

1

0

High-throughput

0.253

Low

501105

HRPA
ATP-dependent RNA helicase HrpA

1

0

High-throughput

0.253

Low

501112

YDIH
Uncharacterized protein YdiH

1

0

High-throughput

0.253

Low

501113

RLMKL
Ribosomal RNA large subunit methyltransferase K/L [Includes: 23S rRNA m2G2445 methyltransferase

1

0

High-throughput

0.249

Low

501114

NPD
NAD-dependent protein deacylase

1

0

High-throughput

0.249

Low

496034

YBGC
Acyl-CoA thioester hydrolase YbgC

1

0

Small-scale

0.232

Low

501116

YUBM
Uncharacterized protein YubM

1

0

High-throughput

0.232

Low


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Last updated: 11Aug2025