Protein: RL3
Large ribosomal subunit protein uL3 (50S ribosomal protein L3)

Gene: RL3, rplC
Organism: Escherichia coli K-12
Interactions: 144
UniProt ID: P60438

Gene Ontology (top 5):
GO:0000027 - ribosomal large subunit assembly
GO:0002181 - cytoplasmic translation
GO:0003735 - structural constituent of ribosome
GO:0005737 - cytoplasm
GO:0005829 - cytosol

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

494333

RS5
Small ribosomal subunit protein uS5

2

2

High-throughput

0.628

High

497390

CSPA
Cold shock protein CspA

2

11

High-throughput

0.612

High

499131

DBHB
DNA-binding protein HU-beta

2

2

High-throughput

0.612

High

788374

RLMN
Dual-specificity RNA methyltransferase RlmN

2

0

High-throughput

0.602

High

491951

RPPH
RNA pyrophosphohydrolase

2

0

High-throughput

0.585

High

494991

TIG
Trigger factor

2

1

Small-scale

0.570

High

491511

IF3
Translation initiation factor IF-3 [Cleaved into: Translation initiation factor IF-3, N-terminally processed; Translation initiation factor IF-3S]

2

0

High-throughput

0.563

High

395169

UVRD
DNA helicase II

1

2

High-throughput

0.536

High

444322

TOP1
DNA topoisomerase 1

1

1

High-throughput

0.536

High

491234

DNAK
Chaperone protein DnaK

1

4

High-throughput

0.536

High

491750

LPXA
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase

1

1

High-throughput

0.536

High

497646

RLUC
Ribosomal large subunit pseudouridine synthase C

1

2

High-throughput

0.536

High

498621

MENB
1,4-dihydroxy-2-naphthoyl-CoA synthase

1

1

High-throughput

0.536

High

501203

RL17
Large ribosomal subunit protein bL17

1

2

High-throughput

0.536

High

569573

SELB
Selenocysteine-specific elongation factor

1

4

High-throughput

0.536

High

578336

HELD
DNA helicase IV

1

2

High-throughput

0.536

High

638581

RNR
Ribonuclease R

1

2

High-throughput

0.536

High

639466

LPXD
UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase

1

1

High-throughput

0.536

High

649700

MUKB
Chromosome partition protein MukB

1

3

High-throughput

0.536

High

676566

MNME
tRNA modification GTPase MnmE

1

6

High-throughput

0.536

High

683076

RHLE
ATP-dependent RNA helicase RhlE

1

4

High-throughput

0.536

High

750367

AIDB
Putative acyl-CoA dehydrogenase AidB

1

1

High-throughput

0.536

High

787524

RSMB
Ribosomal RNA small subunit methyltransferase B

1

3

High-throughput

0.536

High

788392

CSPB
Cold shock-like protein CspB

1

11

High-throughput

0.536

High

790846

RLUB
Ribosomal large subunit pseudouridine synthase B

1

2

High-throughput

0.536

High

827669

ARFB
Peptidyl-tRNA hydrolase ArfB

1

1

High-throughput

0.536

High

950949

RL2
Large ribosomal subunit protein uL2

1

5

High-throughput

0.536

High

951026

MDH
Malate dehydrogenase

1

1

High-throughput

0.536

High

361755

PHSM
Maltodextrin phosphorylase

1

0

High-throughput

0.513

High

368795

SYW
Tryptophan--tRNA ligase

1

0

High-throughput

0.513

High

395090

DNAA
Chromosomal replication initiator protein DnaA

1

0

High-throughput

0.513

High

420217

EX7L
Exodeoxyribonuclease 7 large subunit

1

0

High-throughput

0.513

High

460405

SYL
Leucine--tRNA ligase

1

0

High-throughput

0.513

High

490355

HSLU
ATP-dependent protease ATPase subunit HslU

1

0

High-throughput

0.513

High

492142

HSLV
ATP-dependent protease subunit HslV

1

0

High-throughput

0.513

High

495368

UVRC
UvrABC system protein C

1

0

High-throughput

0.513

High

495997

DARP
Dual-action ribosomal maturation protein DarP

1

0

High-throughput

0.513

High

500338

FKBX
FKBP-type 16 kDa peptidyl-prolyl cis-trans isomerase

1

0

High-throughput

0.513

High

501590

TRKA
Trk system potassium uptake protein TrkA

1

0

High-throughput

0.513

High

522222

DPO3A
DNA polymerase III subunit alpha

1

0

High-throughput

0.513

High

638631

RNE
Ribonuclease E

1

0

High-throughput

0.513

High

653816

ISPA
Farnesyl diphosphate synthase

1

0

High-throughput

0.513

High

663208

PSS
CDP-diacylglycerol--serine O-phosphatidyltransferase PssA

1

0

High-throughput

0.513

High

664133

ACCC
Biotin carboxylase

1

0

High-throughput

0.513

High

785875

CBPA
Curved DNA-binding protein

1

0

High-throughput

0.513

High

790770

RLMJ
Ribosomal RNA large subunit methyltransferase J

1

0

High-throughput

0.513

High

811722

PRMB
Ribosomal protein uL3 glutamine methyltransferase

1

0

High-throughput

0.513

High

811768

RSGA
Small ribosomal subunit biogenesis GTPase RsgA

1

0

High-throughput

0.513

High

951025

RISB
6,7-dimethyl-8-ribityllumazine synthase

1

0

High-throughput

0.513

High

951027

NFUA
Fe/S biogenesis protein NfuA

1

0

High-throughput

0.513

High

951032

GNGF
Putative gluconeogenesis factor

1

0

High-throughput

0.513

High

951034

RLMKL
Ribosomal RNA large subunit methyltransferase K/L [Includes: 23S rRNA m2G2445 methyltransferase

1

0

High-throughput

0.513

High

951040

DCYD
D-cysteine desulfhydrase

1

0

High-throughput

0.513

High

491684

KPRS
Ribose-phosphate pyrophosphokinase

1

1

High-throughput

0.500

High

469386

YCIH
Uncharacterized protein YciH

1

1

High-throughput

0.489

High

571332

TOP3
DNA topoisomerase 3

1

1

High-throughput

0.489

High

578597

SRLR
Glucitol operon repressor

1

1

High-throughput

0.489

High

704175

HOLB
DNA polymerase III subunit delta'

1

1

High-throughput

0.489

High

755437

YEJF
Uncharacterized ABC transporter ATP-binding protein YejF

1

1

High-throughput

0.489

High

490941

IHFA
Integration host factor subunit alpha

1

2

High-throughput

0.484

High

490971

IHFB
Integration host factor subunit beta

1

2

High-throughput

0.484

High

491459

IF2
Translation initiation factor IF-2

1

5

High-throughput

0.484

High

491975

NUSB
Transcription antitermination protein NusB

1

1

High-throughput

0.484

High

492082

IPYR
Inorganic pyrophosphatase

1

1

High-throughput

0.484

High

494646

RPOA
DNA-directed RNA polymerase subunit alpha

1

1

High-throughput

0.484

High

495489

RHLB
ATP-dependent RNA helicase RhlB

1

4

High-throughput

0.484

High

495544

SYT
Threonine--tRNA ligase

1

3

High-throughput

0.484

High

495855

RPOC
DNA-directed RNA polymerase subunit beta'

1

3

High-throughput

0.484

High

496268

CSPD
Cold shock-like protein CspD

1

11

High-throughput

0.484

High

496304

CSPE
Cold shock-like protein CspE

1

11

High-throughput

0.484

High

496320

CSPG
Cold shock-like protein CspG

1

11

High-throughput

0.484

High

496922

LON
Lon protease

1

1

High-throughput

0.484

High

497116

DEAD
ATP-dependent RNA helicase DeaD

1

4

High-throughput

0.484

High

497278

ULAR
HTH-type transcriptional regulator UlaR

1

1

High-throughput

0.484

High

497361

MREB
Cell shape-determining protein MreB

1

5

High-throughput

0.484

High

497437

CSPC
Cold shock-like protein CspC

1

11

High-throughput

0.484

High

498400

CLPA
ATP-dependent Clp protease ATP-binding subunit ClpA

1

5

High-throughput

0.484

High

500750

ODO1
2-oxoglutarate dehydrogenase E1 component

1

3

High-throughput

0.484

High

501616

YFIF
Uncharacterized tRNA/rRNA methyltransferase YfiF

1

4

High-throughput

0.484

High

501887

RLME
Ribosomal RNA large subunit methyltransferase E

1

3

High-throughput

0.484

High

949486

RAPA
RNA polymerase-associated protein RapA

1

4

High-throughput

0.484

High

950223

RSMH
Ribosomal RNA small subunit methyltransferase H

1

1

High-throughput

0.484

High

951024

RL4
Large ribosomal subunit protein uL4

1

3

High-throughput

0.484

High

951028

RLMB
23S rRNA

1

4

High-throughput

0.484

High

951029

CLPB
Chaperone protein ClpB

1

5

High-throughput

0.484

High

490273

CLPP
ATP-dependent Clp protease proteolytic subunit

1

0

High-throughput

0.464

High

490374

COAD
Phosphopantetheine adenylyltransferase

1

0

High-throughput

0.464

High

490496

DTD
D-aminoacyl-tRNA deacylase

1

0

High-throughput

0.464

High

490683

FABZ
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ

1

0

High-throughput

0.464

High

490709

FIS
DNA-binding protein Fis

1

0

High-throughput

0.464

High

490908

HFQ
RNA-binding protein Hfq

1

0

High-throughput

0.464

High

490986

HSLO
33 kDa chaperonin

1

0

High-throughput

0.464

High

491557

KDSA
2-dehydro-3-deoxyphosphooctonate aldolase

1

0

High-throughput

0.464

High

492254

PYRH
Uridylate kinase

1

0

High-throughput

0.464

High

494897

TGT
Queuine tRNA-ribosyltransferase

1

0

High-throughput

0.464

High

495411

YIHI
Der GTPase-activating protein YihI

1

0

High-throughput

0.464

High

496636

LDCI
Inducible lysine decarboxylase

1

0

High-throughput

0.464

High

496736

PHOL
PhoH-like protein

1

0

High-throughput

0.464

High

498002

YBCJ
Putative RNA-binding protein YbcJ

1

0

High-throughput

0.464

High

498276

ACCA
Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha

1

0

High-throughput

0.464

High

499264

CRP
DNA-binding transcriptional dual regulator CRP

1

0

High-throughput

0.464

High

499740

YIBL
Uncharacterized protein YibL

1

0

High-throughput

0.464

High

499954

RL23
Large ribosomal subunit protein uL23

1

0

High-throughput

0.464

High

500710

NUSA
Transcription termination/antitermination protein NusA

1

0

High-throughput

0.464

High

501107

SPOT
Bifunctional

1

0

High-throughput

0.464

High

501141

RHO
Transcription termination factor Rho

1

0

High-throughput

0.464

High

501484

RPOH
RNA polymerase sigma factor RpoH

1

0

High-throughput

0.464

High

501652

YHBY
RNA-binding protein YhbY

1

0

High-throughput

0.464

High

515981

RCSB
Transcriptional regulatory protein RcsB

1

0

High-throughput

0.464

High

951030

HLDD
ADP-L-glycero-D-manno-heptose-6-epimerase

1

0

High-throughput

0.464

High

951031

NFI
Endonuclease V

1

0

High-throughput

0.464

High

501540

SSB
Single-stranded DNA-binding protein

1

1

High-throughput

0.445

High

449917

CCA
Multifunctional CCA protein [Includes: CCA-adding enzyme

1

0

High-throughput

0.279

Low

494762

SFSA
Sugar fermentation stimulation protein A

1

0

High-throughput

0.279

Low

500053

CAIF
Transcriptional activatory protein CaiF

1

0

High-throughput

0.279

Low

501464

SECD
Protein translocase subunit SecD

1

0

High-throughput

0.279

Low

663262

PRIC
Replication restart protein PriC

1

0

High-throughput

0.279

Low

676591

RIBD
Riboflavin biosynthesis protein RibD [Includes: Diaminohydroxyphosphoribosylaminopyrimidine deaminase

1

0

High-throughput

0.279

Low

704180

HOLD
DNA polymerase III subunit psi

1

0

High-throughput

0.279

Low

705894

HOLC
DNA polymerase III subunit chi

1

0

High-throughput

0.279

Low

717664

GREB
Transcription elongation factor GreB

1

0

High-throughput

0.279

Low

760523

HOFQ
DNA utilization protein HofQ

1

0

High-throughput

0.279

Low

852440

YHFX
Uncharacterized protein YhfX

1

0

High-throughput

0.279

Low

861416

RTCB
RNA-splicing ligase RtcB

1

0

High-throughput

0.279

Low

909923

ABPB
Anti-bacteriophage protein B

1

0

High-throughput

0.279

Low

951033

YBJX
Uncharacterized protein YbjX

1

0

High-throughput

0.279

Low

951035

VXIS
Prophage excisionase-like protein

1

0

High-throughput

0.279

Low

951036

PAAX
Transcriptional repressor PaaX

1

0

High-throughput

0.279

Low

951037

RLHA
23S rRNA 5-hydroxycytidine C2501 synthase

1

0

High-throughput

0.279

Low

951038

MCBR
HTH-type transcriptional regulator McbR

1

0

High-throughput

0.279

Low

951039

YNEG
Uncharacterized protein YneG

1

0

High-throughput

0.279

Low

951041

LYSAC
Peptidyl-lysine N-acetyltransferase PatZ

1

0

High-throughput

0.279

Low

951042

DSBG
Thiol:disulfide interchange protein DsbG

1

0

High-throughput

0.279

Low

951043

MURR
HTH-type transcriptional regulator MurR

1

0

High-throughput

0.279

Low

951044

YGCP
Uncharacterized protein YgcP

1

0

High-throughput

0.279

Low

492047

PLSB
Glycerol-3-phosphate acyltransferase

1

0

High-throughput

0.253

Low

494578

RNPA
Ribonuclease P protein component

1

0

High-throughput

0.253

Low

495288

YACL
UPF0231 protein YacL

1

0

High-throughput

0.253

Low

495448

TRMB
tRNA

1

0

High-throughput

0.253

Low

496039

YBGC
Acyl-CoA thioester hydrolase YbgC

1

0

High-throughput

0.253

Low

499688

FIMB
Type 1 fimbriae regulatory protein FimB

1

0

High-throughput

0.253

Low

500058

CEDA
Cell division activator CedA

1

0

High-throughput

0.253

Low

500166

DCM
DNA-cytosine methyltransferase

1

0

High-throughput

0.253

Low

516000

RCSA
Transcriptional regulatory protein RcsA

1

0

High-throughput

0.253

Low


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Last updated: 11Aug2025