Protein: U2AF1
Splicing factor U2AF 35 kDa subunit (U2 auxiliary factor 35 kDa subunit) (U2 small nuclear RNA auxiliary factor 1) (U2 snRNP auxiliary factor small subunit)

Gene: U2AF1
Organism: Homo sapiens
Interactions: 197
UniProt ID: Q01081

Gene Ontology (top 5):
GO:0000398 - mRNA splicing, via spliceosome
GO:0003723 - RNA binding
GO:0005654 - nucleoplasm
GO:0005681 - spliceosomal complex
GO:0006397 - mRNA processing

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

685511

U2AF2
Splicing factor U2AF 65 kDa subunit

17

10

Small-scale

0.988

High

1006340

SRPK2
SRSF protein kinase 2

6

8

High-throughput

0.963

High

1028696

SRPK1
SRSF protein kinase 1

4

7

High-throughput

0.909

High

1028628

SRSF1
Serine/arginine-rich splicing factor 1

4

6

Small-scale

0.890

High

1028649

RNPS1
RNA-binding protein with serine-rich domain 1

3

5

High-throughput

0.837

High

1028686

U2AF4
Splicing factor U2AF 26 kDa subunit

2

6

High-throughput

0.811

High

1028724

ACINU
Apoptotic chromatin condensation inducer in the nucleus

2

1

High-throughput

0.811

High

988087

TRA2B
Transformer-2 protein homolog beta

2

3

High-throughput

0.783

High

1016400

SRSF3
Serine/arginine-rich splicing factor 3

2

7

High-throughput

0.783

High

1028653

U1SBP
U11/U12 small nuclear ribonucleoprotein 35 kDa protein

2

5

High-throughput

0.783

High

1028624

SRSF2
Serine/arginine-rich splicing factor 2

2

1

Small-scale

0.768

High

1028654

LARP7
La-related protein 7

2

3

High-throughput

0.754

High

1028635

SF3B2
Splicing factor 3B subunit 2

1

1

High-throughput

0.740

High

889553

CLK2
Dual specificity protein kinase CLK2

1

3

High-throughput

0.740

High

910463

HNRPM
Heterogeneous nuclear ribonucleoprotein M

1

1

High-throughput

0.724

High

1028650

SF3A2
Splicing factor 3A subunit 2

2

0

Small-scale

0.716

High

891459

GSK3B
Glycogen synthase kinase-3 beta

1

1

High-throughput

0.691

High

1028664

JMJD6
Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6

3

2

High-throughput

0.683

High

134684

SAP18
Histone deacetylase complex subunit SAP18

1

0

High-throughput

0.678

High

329731

ZRAB2
Zinc finger Ran-binding domain-containing protein 2

1

0

Small-scale

0.676

High

92339

D3DU92
RNA-binding protein with serine-rich domain 1

1

5

High-throughput

0.665

High

209249

PRP4
U4/U6 small nuclear ribonucleoprotein Prp4

1

3

High-throughput

0.656

High

550236

PABP1
Polyadenylate-binding protein 1

1

1

High-throughput

0.656

High

1023042

RBM10
RNA-binding protein 10

1

2

High-throughput

0.656

High

1028699

SCAFB
Protein SCAF11

2

1

High-throughput

0.628

High

1028693

TOE1
Target of EGR1 protein 1

1

0

High-throughput

0.622

High

927237

ATX1
Ataxin-1

2

1

High-throughput

0.621

High

1028727

SRPK3
SRSF protein kinase 3

1

8

High-throughput

0.618

High

965627

HNRPK
Heterogeneous nuclear ribonucleoprotein K

2

0

High-throughput

0.618

High

400665

RAF1
RAF proto-oncogene serine/threonine-protein kinase

1

2

High-throughput

0.618

High

431094

LA
Lupus La protein

1

2

High-throughput

0.618

High

472654

RU17
U1 small nuclear ribonucleoprotein 70 kDa

1

5

High-throughput

0.618

High

620301

NUCL
Nucleolin

1

1

High-throughput

0.618

High

1028626

EWS
RNA-binding protein EWS

1

1

High-throughput

0.618

High

1028631

CHD3
Chromodomain-helicase-DNA-binding protein 3

1

1

High-throughput

0.618

High

1028634

G3BP1
Ras GTPase-activating protein-binding protein 1

1

1

High-throughput

0.618

High

1028641

RBM39
RNA-binding protein 39

1

4

High-throughput

0.618

High

1028642

CHD4
Chromodomain-helicase-DNA-binding protein 4

1

1

High-throughput

0.618

High

1028644

SART3
Spliceosome associated factor 3, U4/U6 recycling protein

1

5

High-throughput

0.618

High

1028691

FBXW7
F-box/WD repeat-containing protein 7

1

1

High-throughput

0.618

High

988950

AP2B1
AP-2 complex subunit beta

3

2

High-throughput

0.616

High

1028639

CUL3
Cullin-3

2

1

High-throughput

0.611

High

67822

SRRM4
Serine/arginine repetitive matrix protein 4

2

2

High-throughput

0.606

High

1028637

SNW1
SNW domain-containing protein 1

2

1

Small-scale

0.601

High

1028701

NECD
Necdin

2

0

High-throughput

0.587

High

1028633

TIF1B
Transcription intermediary factor 1-beta

1

0

High-throughput

0.586

High

1028721

ZRAN1
Ubiquitin thioesterase ZRANB1

1

0

High-throughput

0.586

High

1028722

MKRN1
E3 ubiquitin-protein ligase makorin-1

1

0

Small-scale

0.586

High

1028671

CIR1
Corepressor interacting with RBPJ 1

1

1

Small-scale

0.586

High

241420

PRKN
E3 ubiquitin-protein ligase parkin

2

1

High-throughput

0.584

High

371783

MYC
Myc proto-oncogene protein

2

1

High-throughput

0.584

High

1028667

MEPCE
7SK snRNA methylphosphate capping enzyme

2

1

High-throughput

0.584

High

827382

MLH1
DNA mismatch repair protein Mlh1

2

0

High-throughput

0.580

High

1028682

ZN417
Zinc finger protein 417

2

0

High-throughput

0.580

High

687219

HMGB2
High mobility group protein B2

2

0

Small-scale

0.577

High

911554

NCBP2
Nuclear cap-binding protein subunit 2

1

1

High-throughput

0.575

High

1028651

SF01
Splicing factor 1

1

1

High-throughput

0.575

High

216418

SUV91
Histone-lysine N-methyltransferase SUV39H1

1

1

High-throughput

0.573

High

951618

PTEN
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN

2

0

Small-scale

0.569

High

830576

CCNF
Cyclin-F

2

0

High-throughput

0.559

High

979509

H4
Histone H4

2

0

High-throughput

0.559

High

407430

K2C1
Keratin, type II cytoskeletal 1

2

1

High-throughput

0.553

High

1028630

AP1B1
AP-1 complex subunit beta-1

2

2

High-throughput

0.553

High

891118

GSK3A
Glycogen synthase kinase-3 alpha

1

2

High-throughput

0.553

High

1028676

MINK1
Misshapen-like kinase 1

1

1

High-throughput

0.553

High

1028678

NKAP
NF-kappa-B-activating protein

1

0

High-throughput

0.549

High

1028681

RP9
Retinitis pigmentosa 9 protein

1

0

Small-scale

0.549

High

1028658

LRRK2
Leucine-rich repeat serine/threonine-protein kinase 2

1

0

High-throughput

0.546

High

1028713

LN28A
Protein lin-28 homolog A

1

0

High-throughput

0.546

High

765398

BMI1
Polycomb complex protein BMI-1

1

1

High-throughput

0.536

High

1028638

CUL1
Cullin-1

1

1

High-throughput

0.536

High

1028670

CAND1
Cullin-associated NEDD8-dissociated protein 1

1

1

High-throughput

0.536

High

1028675

CLASR
CLK4-associating serine/arginine rich protein

1

1

High-throughput

0.536

High

1028697

CDC5L
Cell division cycle 5-like protein

1

2

High-throughput

0.536

High

1028645

SUZ12
Polycomb protein SUZ12

1

1

High-throughput

0.515

High

908378

HDGF
Hepatoma-derived growth factor

1

0

High-throughput

0.513

High

1028662

IFIX
Pyrin and HIN domain-containing protein 1

1

0

High-throughput

0.513

High

1028700

TSNAX
Translin-associated protein X

1

0

High-throughput

0.513

High

280585

PR40A
Pre-mRNA-processing factor 40 homolog A

1

4

High-throughput

0.508

High

935490

MFAP1
Microfibrillar-associated protein 1

1

1

High-throughput

0.508

High

974359

SMD1
Small nuclear ribonucleoprotein Sm D1

1

3

High-throughput

0.508

High

1028672

CHERP
Calcium homeostasis endoplasmic reticulum protein

1

3

High-throughput

0.508

High

1028726

PRP19
Pre-mRNA-processing factor 19

1

4

High-throughput

0.508

High

1028720

NXF1
Nuclear RNA export factor 1

1

0

Small-scale

0.504

High

843490

HD
Huntingtin

1

0

High-throughput

0.493

High

1028666

F10C1
Protein FRA10AC1

1

0

High-throughput

0.486

High

1028679

PR38A
Pre-mRNA-splicing factor 38A

1

0

High-throughput

0.486

High

1028684

ZCH10
Zinc finger CCHC domain-containing protein 10

1

0

High-throughput

0.486

High

1028690

PRP16
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

1

0

High-throughput

0.486

High

893824

MEOX2
Homeobox protein MOX-2

1

1

High-throughput

0.481

High

1028668

CPEB2
Cytoplasmic polyadenylation element-binding protein 2

1

1

High-throughput

0.481

High

259806

BRD4
Bromodomain-containing protein 4

1

2

High-throughput

0.479

High

315474

RECQ4
ATP-dependent DNA helicase Q4

1

1

High-throughput

0.479

High

324801

HEXI1
Protein HEXIM1

1

1

High-throughput

0.479

High

325789

UBR5
E3 ubiquitin-protein ligase UBR5

1

3

High-throughput

0.479

High

342831

HERC2
E3 ubiquitin-protein ligase HERC2

1

3

High-throughput

0.479

High

406076

MYCN
N-myc proto-oncogene protein

1

1

High-throughput

0.479

High

445531

IF4E
Eukaryotic translation initiation factor 4E

1

1

Small-scale

0.479

High

459545

PROF1
Profilin-1

1

1

Small-scale

0.479

High

521314

ARAF
Serine/threonine-protein kinase A-Raf

1

2

High-throughput

0.479

High

854828

RECQ1
ATP-dependent DNA helicase Q1

1

1

High-throughput

0.479

High

904608

MECP2
Methyl-CpG-binding protein 2

1

1

High-throughput

0.479

High

964856

WDR5
WD repeat-containing protein 5

1

2

High-throughput

0.479

High

1026318

HSF1
Heat shock factor protein 1

1

1

High-throughput

0.479

High

1028627

KIF23
Kinesin-like protein KIF23

1

2

High-throughput

0.479

High

1028629

EP300
Histone acetyltransferase p300

1

1

High-throughput

0.479

High

1028647

U5S1
116 kDa U5 small nuclear ribonucleoprotein component

1

1

High-throughput

0.479

High

1028652

EZH2
Histone-lysine N-methyltransferase EZH2

1

3

High-throughput

0.479

High

1028665

TRI67
Tripartite motif-containing protein 67

1

1

High-throughput

0.479

High

1028669

ZCH18
Zinc finger CCCH domain-containing protein 18

1

1

High-throughput

0.479

High

1028673

SMC5
Structural maintenance of chromosomes protein 5

1

1

High-throughput

0.479

High

1028698

RING2
E3 ubiquitin-protein ligase RING2

1

2

High-throughput

0.479

High

1028706

DDX23
Probable ATP-dependent RNA helicase DDX23

1

1

High-throughput

0.479

High

1028708

TRI31
E3 ubiquitin-protein ligase TRIM31

1

1

High-throughput

0.479

High

1028710

DDX24
ATP-dependent RNA helicase DDX24

1

1

High-throughput

0.479

High

1028715

SMUF1
E3 ubiquitin-protein ligase SMURF1

1

2

High-throughput

0.479

High

1028717

BRD7
Bromodomain-containing protein 7

1

2

High-throughput

0.479

High

1028718

ANLN
Anillin

1

1

High-throughput

0.479

High

1028725

FZR1
Fizzy-related protein homolog

1

3

High-throughput

0.479

High

1028661

Q6FHY5
MEOX2 protein

1

1

High-throughput

0.470

High

1028703

HINFP
Histone H4 transcription factor

1

0

Small-scale

0.460

High

140872

NOP56
Nucleolar protein 56

1

0

Small-scale

0.458

High

222949

PRC1
Protein regulator of cytokinesis 1

1

0

High-throughput

0.458

High

247830

NUPR1
Nuclear protein 1

1

0

High-throughput

0.458

High

349952

SNAI1
Zinc finger protein SNAI1

1

0

High-throughput

0.458

High

352402

TOP3B
DNA topoisomerase 3-beta-1

1

0

High-throughput

0.458

High

588483

RFA2
Replication protein A 32 kDa subunit

1

0

High-throughput

0.458

High

590877

TIMP2
Metalloproteinase inhibitor 2

1

0

High-throughput

0.458

High

606638

CEBPB
CCAAT/enhancer-binding protein beta

1

0

High-throughput

0.458

High

731207

STIP1
Stress-induced-phosphoprotein 1

1

0

High-throughput

0.458

High

754803

H2B1B
Histone H2B type 1-B

1

0

High-throughput

0.458

High

766458

PHB1
Prohibitin 1

1

0

High-throughput

0.458

High

878976

NR2C2
Nuclear receptor subfamily 2 group C member 2

1

0

High-throughput

0.458

High

928417

TERF1
Telomeric repeat-binding factor 1

1

0

Small-scale

0.458

High

960400

PSME3
Proteasome activator complex subunit 3

1

0

Small-scale

0.458

High

1013128

SARNP
SAP domain-containing ribonucleoprotein

1

0

Small-scale

0.458

High

1016778

H33
Histone H3.3

1

0

High-throughput

0.458

High

1024509

VIGLN
Vigilin

1

0

High-throughput

0.458

High

1028632

CACO2
Calcium-binding and coiled-coil domain-containing protein 2

1

0

High-throughput

0.458

High

1028636

BIRC3
Baculoviral IAP repeat-containing protein 3

1

0

High-throughput

0.458

High

1028646

TNIP1
TNFAIP3-interacting protein 1

1

0

High-throughput

0.458

High

1028660

RNF43
E3 ubiquitin-protein ligase RNF43

1

0

High-throughput

0.458

High

1028663

ZN746
Zinc finger protein 746

1

0

High-throughput

0.458

High

1028680

SERB1
SERPINE1 mRNA-binding protein 1

1

0

High-throughput

0.458

High

1028685

DOT1L
Histone-lysine N-methyltransferase, H3 lysine-79 specific

1

0

High-throughput

0.458

High

1028689

BAP1
Ubiquitin carboxyl-terminal hydrolase BAP1

1

0

High-throughput

0.458

High

1028692

DCPS
m7GpppX diphosphatase

1

0

High-throughput

0.458

High

1028702

BARD1
BRCA1-associated RING domain protein 1

1

0

High-throughput

0.458

High

1028704

OVOL2
Transcription factor Ovo-like 2

1

0

Small-scale

0.458

High

1028705

RIOK1
Serine/threonine-protein kinase RIO1

1

0

High-throughput

0.458

High

1028707

ACE2
Angiotensin-converting enzyme 2

1

0

High-throughput

0.458

High

1028712

ECT2
Protein ECT2

1

0

High-throughput

0.458

High

1028714

NMRL1
NmrA-like family domain-containing protein 1

1

0

High-throughput

0.458

High

1028719

AATF
Protein AATF

1

0

High-throughput

0.458

High

1028728

AAR2
Protein AAR2 homolog

1

0

High-throughput

0.458

High

1028729

CFA20
Cilia- and flagella-associated protein 20

1

0

Small-scale

0.458

High

131963

WWP2
NEDD4-like E3 ubiquitin-protein ligase WWP2

1

2

High-throughput

0.437

High

1028643

CUL7
Cullin-7

1

1

High-throughput

0.437

High

1028648

KIF14
Kinesin-like protein KIF14

1

1

High-throughput

0.437

High

529643

TAU
Microtubule-associated protein tau

1

0

High-throughput

0.426

High

1028656

NCBP3
Nuclear cap-binding protein subunit 3

1

0

High-throughput

0.426

High

1028709

ZDHC5
Palmitoyltransferase ZDHHC5

1

0

High-throughput

0.426

High

1028723

DDX41
Probable ATP-dependent RNA helicase DDX41

1

0

High-throughput

0.426

High

327949

GBRAP
Gamma-aminobutyric acid receptor-associated protein

1

1

High-throughput

0.407

High

952107

GBRL2
Gamma-aminobutyric acid receptor-associated protein-like 2

1

1

High-throughput

0.407

High

1028677

CARME
Carnosine N-methyltransferase

4

0

High-throughput

0.364

High

341756

K2C75
Keratin, type II cytoskeletal 75

2

0

High-throughput

0.316

High

1028657

EFHC2
EF-hand domain-containing family member C2

2

0

High-throughput

0.316

High

557235

SRC
Proto-oncogene tyrosine-protein kinase Src

2

0

High-throughput

0.298

High

171064

VP26C
Vacuolar protein sorting-associated protein 26C

1

0

High-throughput

0.282

High

585450

VEGFA
Vascular endothelial growth factor A, long form

1

0

High-throughput

0.282

High

389299

FINC
Fibronectin

1

0

High-throughput

0.279

Low

515824

RGPD2
RANBP2-like and GRIP domain-containing protein 2

1

0

High-throughput

0.279

Low

949234

MYPR
Myelin proteolipid protein

1

0

High-throughput

0.279

Low

1028716

ALG13
UDP-N-acetylglucosamine transferase subunit ALG13

1

0

High-throughput

0.251

Low

272083

OBSL1
Obscurin-like protein 1

1

0

High-throughput

0.249

Low

293575

CD123
Translation initiation factor eIF2 assembly protein

1

0

High-throughput

0.249

Low

318730

RHBT1
Rho-related BTB domain-containing protein 1

1

0

High-throughput

0.249

Low

319680

UFL1
E3 UFM1-protein ligase 1

1

0

High-throughput

0.249

Low

411751

NTRK1
High affinity nerve growth factor receptor

1

0

High-throughput

0.249

Low

418943

H2A1B
Histone H2A type 1-B/E

1

0

High-throughput

0.249

Low

482439

F16P1
Fructose-1,6-bisphosphatase 1

1

0

High-throughput

0.249

Low

583289

FOLR1
Folate receptor alpha

1

0

High-throughput

0.249

Low

774293

BASI
Basigin

1

0

High-throughput

0.249

Low

970348

UBE2H
Ubiquitin-conjugating enzyme E2 H

1

0

High-throughput

0.249

Low

995542

ACTC
Actin, alpha cardiac muscle 1

1

0

High-throughput

0.249

Low

1028625

IFM3
Interferon-induced transmembrane protein 3

1

0

High-throughput

0.249

Low

1028640

GRM2
Metabotropic glutamate receptor 2

1

0

High-throughput

0.249

Low

1028655

MIDN
Midnolin

1

0

High-throughput

0.249

Low

1028674

MILK2
MICAL-like protein 2

1

0

High-throughput

0.249

Low

1028683

TIP
T-cell immunomodulatory protein

1

0

High-throughput

0.249

Low

1028687

CLC4D
C-type lectin domain family 4 member D

1

0

High-throughput

0.249

Low

1028688

SMG7
Nonsense-mediated mRNA decay factor SMG7

1

0

High-throughput

0.249

Low

1028694

DDRGK
DDRGK domain-containing protein 1

1

0

High-throughput

0.249

Low

1028695

CI072
Guanine nucleotide exchange factor C9orf72

1

0

High-throughput

0.249

Low

1028711

PKHA4
Pleckstrin homology domain-containing family A member 4

1

0

High-throughput

0.249

Low

1028659

LITD1
LINE-1 type transposase domain-containing protein 1

1

0

High-throughput

0.232

Low


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Last updated: 11Aug2025