Protein: MED4
Mediator of RNA polymerase II transcription subunit 4 (Mediator complex subunit 4)

Gene: MED4
Organism: Saccharomyces cerevisiae S288C
Interactions: 95
UniProt ID: Q12343

Gene Ontology (top 5):
GO:0000979 - RNA polymerase II core promoter sequence-specific DNA binding
GO:0003712 - transcription coregulator activity
GO:0005634 - nucleus
GO:0006357 - regulation of transcription by RNA polymerase II
GO:0006366 - transcription by RNA polymerase II

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

1062901

MED7
Mediator of RNA polymerase II transcription subunit 7

16

1

Small-scale

0.984

High

751223

MED9
Mediator of RNA polymerase II transcription subunit 9

10

0

Small-scale

0.925

High

740444

MED17
Mediator of RNA polymerase II transcription subunit 17

10

0

Small-scale

0.921

High

1084756

MED1
Mediator of RNA polymerase II transcription subunit 1

9

0

Small-scale

0.917

High

619455

MED14
Mediator of RNA polymerase II transcription subunit 14

7

0

Small-scale

0.910

High

867884

MED21
Mediator of RNA polymerase II transcription subunit 21

7

0

Small-scale

0.910

High

801170

MED31
Mediator of RNA polymerase II transcription subunit 31

5

0

Small-scale

0.872

High

797727

MED8
Mediator of RNA polymerase II transcription subunit 8

7

1

Small-scale

0.735

High

740820

MED18
Mediator of RNA polymerase II transcription subunit 18

9

0

Small-scale

0.717

High

1084340

MED2
Mediator of RNA polymerase II transcription subunit 2

6

0

Small-scale

0.691

High

823239

MED3
Mediator of RNA polymerase II transcription subunit 3

5

0

Small-scale

0.684

High

740481

MED22
Mediator of RNA polymerase II transcription subunit 22

5

0

Small-scale

0.682

High

668505

MED19
Mediator of RNA polymerase II transcription subunit 19

5

0

Small-scale

0.679

High

1054360

MED10
Mediator of RNA polymerase II transcription subunit 10

5

0

Small-scale

0.676

High

624955

MED15
Mediator of RNA polymerase II transcription subunit 15

4

0

Small-scale

0.661

High

917296

MED5
Mediator of RNA polymerase II transcription subunit 5

4

0

Small-scale

0.661

High

804038

MED6
Mediator of RNA polymerase II transcription subunit 6

4

0

Small-scale

0.649

High

810680

SSN3
Meiotic mRNA stability protein kinase SSN3

3

0

Small-scale

0.619

High

546799

SSB1
Ribosome-associated molecular chaperone SSB1

2

1

High-throughput

0.584

High

733570

MED16
Mediator of RNA polymerase II transcription subunit 16

2

0

High-throughput

0.576

High

757603

MED20
Mediator of RNA polymerase II transcription subunit 20

2

0

High-throughput

0.576

High

440872

KIN28
Serine/threonine-protein kinase KIN28

2

0

Small-scale

0.569

High

807862

SSN2
Mediator of RNA polymerase II transcription subunit 13

2

0

Small-scale

0.569

High

548845

TAF13
Transcription initiation factor TFIID subunit 13

2

0

High-throughput

0.563

High

1059358

RXT3
Transcriptional regulatory protein RXT3

2

0

High-throughput

0.563

High

402153

RPB1
DNA-directed RNA polymerase II subunit RPB1

2

0

Small-scale

0.559

High

594643

RPB3
DNA-directed RNA polymerase II subunit RPB3

2

0

Small-scale

0.559

High

577588

CYC8
General transcriptional corepressor CYC8

1

0

Small-scale

0.513

High

722367

XPO1
Exportin-1

1

0

High-throughput

0.513

High

1043993

TAF12
SAGA complex/transcription factor TFIID complex subunit TAF12

1

0

High-throughput

0.513

High

793585

TBS1
Uncharacterized transcriptional regulatory protein TBS1

1

1

High-throughput

0.508

High

680172

CDC48
Cell division control protein 48

1

0

High-throughput

0.493

High

404993

PUR3
Phosphoribosylglycinamide formyltransferase

1

0

High-throughput

0.486

High

824890

NDC80
Kinetochore protein NDC80

1

0

High-throughput

0.486

High

1056583

SWC7
SWR1-complex protein 7

1

0

High-throughput

0.486

High

760640

TSA1
Peroxiredoxin TSA1

1

0

High-throughput

0.479

High

408449

GAL4
Regulatory protein GAL4

1

1

Small-scale

0.479

High

864146

LSM1
LSM1-LSM7 complex subunit LSM1

1

1

Small-scale

0.479

High

865292

LSM8
LSM2-LSM8 complex subunit LSM8

1

1

Small-scale

0.479

High

557791

SRS2
ATP-dependent DNA helicase SRS2

1

0

Small-scale

0.460

High

699434

CIN8
Kinesin-like protein CIN8

1

0

High-throughput

0.460

High

395270

GCN4
General control transcription factor GCN4

1

0

Small-scale

0.458

High

560251

TOP3
DNA topoisomerase 3

1

0

High-throughput

0.458

High

580268

HSC82
ATP-dependent molecular chaperone HSC82

1

0

High-throughput

0.458

High

678823

SRB8
Mediator of RNA polymerase II transcription subunit 12

1

0

High-throughput

0.458

High

708571

TF2B
Transcription initiation factor IIB

1

0

High-throughput

0.458

High

734496

BTAF1
TATA-binding protein-associated factor MOT1

1

0

Small-scale

0.458

High

763400

TAF14
Transcription initiation factor TFIID subunit 14

1

0

High-throughput

0.458

High

776677

DEF1
RNA polymerase II degradation factor 1

1

0

High-throughput

0.458

High

800784

RCK1
Serine/threonine-protein kinase RCK1

1

0

High-throughput

0.458

High

826416

MPH1
ATP-dependent DNA helicase MPH1

1

0

High-throughput

0.458

High

865799

MOG1
Nuclear import protein MOG1

1

0

High-throughput

0.458

High

867868

SSN8
RNA polymerase II holoenzyme cyclin-like subunit

1

0

High-throughput

0.458

High

925150

APJ1
J domain-containing protein APJ1

1

0

High-throughput

0.458

High

1084151

TAF10
SAGA complex/transcription factor TFIID complex subunit TAF10

1

0

High-throughput

0.458

High

1084803

SMC6
Structural maintenance of chromosomes protein 6

1

0

High-throughput

0.458

High

1084804

MED11
Mediator of RNA polymerase II transcription subunit 11

1

0

High-throughput

0.458

High

742712

HSP7F
Heat shock protein homolog SSE1

1

1

High-throughput

0.445

High

820156

SSB2
Ribosome-associated molecular chaperone SSB2

1

1

High-throughput

0.445

High

806681

RIX1
Pre-rRNA-processing protein RIX1

1

0

High-throughput

0.438

High

1084404

PER33
Pore and endoplasmic reticulum protein of 33 kDa

1

1

Small-scale

0.437

High

1084520

POM33
Pore membrane protein of 33 kDa

1

1

Small-scale

0.437

High

381317

H2B1
Histone H2B.1

1

0

High-throughput

0.426

High

528055

HSP72
Heat shock protein SSA2

1

0

High-throughput

0.426

High

671256

SIS1
Protein SIS1

1

0

High-throughput

0.426

High

804570

SSZ1
Ribosome-associated complex subunit SSZ1

1

0

High-throughput

0.426

High

1042404

GCN5
Histone acetyltransferase GCN5

1

0

High-throughput

0.426

High

1084573

RIO1
Serine/threonine-protein kinase RIO1

1

0

High-throughput

0.426

High

510912

HSP77
Import motor subunit, mitochondrial

1

1

High-throughput

0.407

High

816478

HSP7E
Heat shock protein SSC3, mitochondrial

1

1

High-throughput

0.407

High

871026

SNAPN
Biogenesis of lysosome-related organelles complex 1 subunit SNN1

2

0

High-throughput

0.307

High

923043

ZRG17
Protein ZRG17

1

0

High-throughput

0.279

Low

1041737

YMZ2
WD repeat-containing protein YMR102C

1

0

High-throughput

0.279

Low

782231

GPT2
Glycerol-3-phosphate O-acyltransferase 2

1

0

High-throughput

0.269

Low

811685

DSS1
Exoribonuclease II, mitochondrial

1

0

High-throughput

0.269

Low

830079

CIN1
Chromosome instability protein 1

1

0

High-throughput

0.269

Low

1018310

SEC5
Exocyst complex component SEC5

1

0

High-throughput

0.269

Low

743733

VATD
V-type proton ATPase subunit D

1

0

High-throughput

0.265

Low

813977

RBG1
Ribosome-interacting GTPase 1

1

0

High-throughput

0.265

Low

367828

ENO1
Enolase 1

1

0

High-throughput

0.261

Low

439174

ILV5
Ketol-acid reductoisomerase, mitochondrial

1

0

High-throughput

0.261

Low

456469

ILVB
Acetolactate synthase catalytic subunit, mitochondrial

1

0

High-throughput

0.261

Low

572669

ALF
Fructose-bisphosphate aldolase

1

0

High-throughput

0.261

Low

603574

VATA
V-type proton ATPase catalytic subunit A

1

0

High-throughput

0.261

Low

626927

HSP60
Heat shock protein 60, mitochondrial

1

0

High-throughput

0.261

Low

410401

RL25
Large ribosomal subunit protein uL23

1

0

High-throughput

0.249

Low

442177

RS14A
Small ribosomal subunit protein uS11A

1

0

High-throughput

0.249

Low

747643

RSSA1
Small ribosomal subunit protein uS2A

1

0

High-throughput

0.249

Low

812779

LAA1
AP-1 accessory protein LAA1

1

0

High-throughput

0.249

Low

865341

MTNB
Methylthioribulose-1-phosphate dehydratase

1

0

High-throughput

0.249

Low

874595

RS12
Small ribosomal subunit protein eS12

1

0

High-throughput

0.249

Low

1033635

RS15
Small ribosomal subunit protein uS19

1

0

High-throughput

0.249

Low

671346

CANB
Calcineurin subunit B

1

0

High-throughput

0.238

Low

1056459

SWA2
Auxilin-like clathrin uncoating factor SWA2

1

0

High-throughput

0.232

Low

1084763

HSP42
Heat shock protein 42

1

0

High-throughput

0.232

Low


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Last updated: 11Aug2025