Protein: MYLK
Myosin light chain kinase, smooth muscle (MLCK) (smMLCK) (EC 2.7.11.18) (Kinase-related protein) (KRP) (Telokin) [Cleaved into: Myosin light chain kinase, smooth muscle, deglutamylated form]

Gene: MYLK
Organism: Homo sapiens
Interactions: 109
UniProt ID: Q15746

Gene Ontology (top 5):
GO:0001725 - stress fiber
GO:0003779 - actin binding
GO:0004687 - myosin light chain kinase activity
GO:0005516 - calmodulin binding
GO:0005524 - ATP binding

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

1153022

GOPC
Golgi-associated PDZ and coiled-coil motif-containing protein

3

20

High-throughput

0.871

High

1153021

MYLK2
Myosin light chain kinase 2, skeletal/cardiac muscle

2

16

High-throughput

0.737

High

1125450

FKBP8
Peptidyl-prolyl cis-trans isomerase FKBP8

1

6

Small-scale

0.713

High

1096441

PAK1
Serine/threonine-protein kinase PAK 1

1

4

Small-scale

0.710

High

955204

CDC42
Cell division control protein 42 homolog

1

9

High-throughput

0.691

High

956728

RAB10
Ras-related protein Rab-10

1

4

High-throughput

0.691

High

1038627

FKBP4
Peptidyl-prolyl cis-trans isomerase FKBP4

1

7

High-throughput

0.691

High

1153024

ANLN
Anillin

1

3

High-throughput

0.691

High

1153015

ROAA
Heterogeneous nuclear ribonucleoprotein A/B

1

10

High-throughput

0.665

High

1153018

OSBL8
Oxysterol-binding protein-related protein 8

1

0

High-throughput

0.656

High

1098875

KGP2
cGMP-dependent protein kinase 2

1

4

Small-scale

0.647

High

169095

ML12B
Myosin regulatory light chain 12B

1

18

High-throughput

0.618

High

171248

MYPT1
Protein phosphatase 1 regulatory subunit 12A

1

11

High-throughput

0.618

High

241783

PRKN
E3 ubiquitin-protein ligase parkin

1

8

High-throughput

0.618

High

557323

SRC
Proto-oncogene tyrosine-protein kinase Src

1

9

Small-scale

0.618

High

594010

NCK1
SH2/SH3 adapter protein NCK1

1

11

High-throughput

0.618

High

666754

MYL9
Myosin regulatory light polypeptide 9

1

18

High-throughput

0.618

High

699766

P85A
Phosphatidylinositol 3-kinase regulatory subunit alpha

1

5

High-throughput

0.618

High

987664

GRB2
Growth factor receptor-bound protein 2

1

14

High-throughput

0.618

High

1153006

MPRIP
Myosin phosphatase Rho-interacting protein

1

11

High-throughput

0.618

High

1153019

UACA
Uveal autoantigen with coiled-coil domains and ankyrin repeats

1

13

High-throughput

0.618

High

1153027

ALK
ALK tyrosine kinase receptor

1

12

Small-scale

0.618

High

1153010

APBP2
Amyloid protein-binding protein 2

2

4

High-throughput

0.606

High

618693

ML12A
Myosin regulatory light chain 12A

1

19

Small-scale

0.575

High

1097200

PAK2
Serine/threonine-protein kinase PAK 2

1

3

Small-scale

0.575

High

1110447

KCC2D
Calcium/calmodulin-dependent protein kinase type II subunit delta

1

17

High-throughput

0.575

High

415593

HSPB1
Heat shock protein beta-1

1

11

High-throughput

0.541

High

123393

BACH
Cytosolic acyl coenzyme A thioester hydrolase

1

1

High-throughput

0.536

High

365388

PGK1
Phosphoglycerate kinase 1

1

2

High-throughput

0.536

High

452269

PDIA1
Protein disulfide-isomerase

1

6

High-throughput

0.536

High

476154

ANXA5
Annexin A5

1

1

High-throughput

0.536

High

532224

ESTD
S-formylglutathione hydrolase

1

1

High-throughput

0.536

High

582811

EZRI
Ezrin

1

16

High-throughput

0.536

High

592845

CBR1
Carbonyl reductase [NADPH] 1

1

1

High-throughput

0.536

High

646850

PUR6
Bifunctional phosphoribosylaminoimidazole carboxylase/phosphoribosylaminoimidazole succinocarboxamide synthetase

1

1

High-throughput

0.536

High

647560

UBA1
Ubiquitin-like modifier-activating enzyme 1

1

4

High-throughput

0.536

High

687680

SYTC
Threonine--tRNA ligase 1, cytoplasmic

1

3

High-throughput

0.536

High

712213

TKT
Transketolase

1

3

High-throughput

0.536

High

716297

PRDX6
Peroxiredoxin-6

1

2

High-throughput

0.536

High

722712

AL1B1
Aldehyde dehydrogenase X, mitochondrial

1

1

High-throughput

0.536

High

760926

GLYM
Serine hydroxymethyltransferase, mitochondrial

1

2

High-throughput

0.536

High

772822

MYH9
Myosin-9

1

6

High-throughput

0.536

High

828782

MDHM
Malate dehydrogenase, mitochondrial

1

3

High-throughput

0.536

High

867158

CAPZB
F-actin-capping protein subunit beta

1

2

High-throughput

0.536

High

881052

NASP
Nuclear autoantigenic sperm protein

1

7

High-throughput

0.536

High

910309

6PGD
6-phosphogluconate dehydrogenase, decarboxylating

1

1

High-throughput

0.536

High

912570

GDIR1
Rho GDP-dissociation inhibitor 1

1

1

High-throughput

0.536

High

920812

ACLY
ATP-citrate synthase

1

1

High-throughput

0.536

High

968317

PP1B
Serine/threonine-protein phosphatase PP1-beta catalytic subunit

1

6

High-throughput

0.536

High

992586

IF5A1
Eukaryotic translation initiation factor 5A-1

1

2

High-throughput

0.536

High

1008168

PRKDC
DNA-dependent protein kinase catalytic subunit

1

1

High-throughput

0.536

High

1092545

ECH1
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

1

2

High-throughput

0.536

High

1142276

PDIA6
Protein disulfide-isomerase A6

1

7

High-throughput

0.536

High

1144001

TEBP
Prostaglandin E synthase 3

1

1

High-throughput

0.536

High

1153001

AAAT
Neutral amino acid transporter B(0)

1

1

High-throughput

0.536

High

1153013

MYO19
Unconventional myosin-XIX

1

15

High-throughput

0.536

High

1153017

TX101
Testis-expressed protein 101

1

1

High-throughput

0.536

High

1153025

XPP1
Xaa-Pro aminopeptidase 1

1

1

High-throughput

0.536

High

1153028

CHIP
E3 ubiquitin-protein ligase CHIP

1

6

High-throughput

0.536

High

1153030

MYO5A
Unconventional myosin-Va

1

15

High-throughput

0.536

High

105991

APRIO
Alternative prion protein

2

6

High-throughput

0.533

High

1153014

ZBT18
Zinc finger and BTB domain-containing protein 18

1

3

High-throughput

0.514

High

130113

PGRC1
Membrane-associated progesterone receptor component 1

1

0

High-throughput

0.513

High

362041

AATM
Aspartate aminotransferase, mitochondrial

1

0

High-throughput

0.513

High

405289

OAT
Ornithine aminotransferase, mitochondrial

1

0

High-throughput

0.513

High

641785

SDHB
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial

1

0

High-throughput

0.513

High

657373

MAOM
NAD-dependent malic enzyme, mitochondrial

1

0

High-throughput

0.513

High

728947

PUR9
Bifunctional purine biosynthesis protein ATIC

1

0

High-throughput

0.513

High

834314

GARS
Glycine--tRNA ligase

1

0

High-throughput

0.513

High

894552

GDIB
Rab GDP dissociation inhibitor beta

1

0

High-throughput

0.513

High

938568

PSA
Puromycin-sensitive aminopeptidase

1

0

High-throughput

0.513

High

1148976

SC23B
Protein transport protein Sec23B

1

0

High-throughput

0.513

High

1153004

TSR1
Pre-rRNA-processing protein TSR1 homolog

1

0

High-throughput

0.513

High

1153023

INO1
Inositol-3-phosphate synthase 1

1

0

High-throughput

0.513

High

1153029

DRG1
Developmentally-regulated GTP-binding protein 1

1

0

High-throughput

0.513

High

1153031

SERC
Phosphoserine aminotransferase

1

0

High-throughput

0.513

High

1124602

SRC8
Src substrate cortactin

1

8

Small-scale

0.501

High

423909

ALDH2
Aldehyde dehydrogenase, mitochondrial

1

1

High-throughput

0.489

High

571440

GLU2B
Glucosidase 2 subunit beta

1

2

High-throughput

0.489

High

666301

THIL
Acetyl-CoA acetyltransferase, mitochondrial

1

1

High-throughput

0.489

High

698688

CALX
Calnexin

1

6

High-throughput

0.489

High

1153007

TXND5
Thioredoxin domain-containing protein 5

1

7

High-throughput

0.489

High

1153011

KCD12
BTB/POZ domain-containing protein KCTD12

1

8

High-throughput

0.489

High

1153012

TRM61
tRNA

1

1

High-throughput

0.489

High

1139901

MLC1
Membrane protein MLC1

1

0

Small-scale

0.480

High

413904

P53
Cellular tumor antigen p53

1

4

Small-scale

0.479

High

754858

H2B1B
Histone H2B type 1-B

1

11

High-throughput

0.479

High

952567

MYL6
Myosin light polypeptide 6

1

12

High-throughput

0.479

High

970567

UBE2H
Ubiquitin-conjugating enzyme E2 H

1

9

High-throughput

0.479

High

1153005

C2D1B
Coiled-coil and C2 domain-containing protein 1B

1

6

High-throughput

0.479

High

1153009

PRP16
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

1

1

High-throughput

0.479

High

1153026

SEPT9
Septin-9

1

1

Small-scale

0.479

High

840715

RS27
Small ribosomal subunit protein eS27

1

0

High-throughput

0.458

High

954141

RS20
Small ribosomal subunit protein uS10

1

0

High-throughput

0.458

High

1153002

E2F4
Transcription factor E2F4

1

0

High-throughput

0.458

High

404804

PRIO
Major prion protein

1

4

High-throughput

0.445

High

765549

BMI1
Polycomb complex protein BMI-1

1

10

High-throughput

0.445

High

1103189

CTBP1
C-terminal-binding protein 1

1

4

High-throughput

0.445

High

588792

RFA2
Replication protein A 32 kDa subunit

1

1

High-throughput

0.437

High

695557

RFA1
Replication protein A 70 kDa DNA-binding subunit

1

2

High-throughput

0.437

High

768285

RFA3
Replication protein A 14 kDa subunit

1

1

High-throughput

0.437

High

964971

WDR5
WD repeat-containing protein 5

1

4

High-throughput

0.437

High

1153003

CCD27
Coiled-coil domain-containing protein 27

1

10

High-throughput

0.437

High

1153008

RHBL4
Rhomboid-related protein 4

1

2

High-throughput

0.437

High

429952

JUN
Transcription factor Jun

1

7

High-throughput

0.407

High

1153020

LACRT
Extracellular glycoprotein lacritin

1

0

High-throughput

0.279

Low

66716

D42E2
Putative short-chain dehydrogenase/reductase family 42E member 2

1

0

High-throughput

0.249

Low

1119622

CNCG
Retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma

1

0

High-throughput

0.249

Low

1153016

RT63
Large ribosomal subunit protein mL63

1

0

High-throughput

0.249

Low


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Last updated: 11Aug2025