Protein: MYLK2
Myosin light chain kinase 2, skeletal/cardiac muscle (MLCK2) (EC 2.7.11.18)

Gene: MYLK2
Organism: Homo sapiens
Interactions: 114
UniProt ID: Q9H1R3

Gene Ontology (top 5):
GO:0004683 - calcium/calmodulin-dependent protein kinase activity
GO:0004687 - myosin light chain kinase activity
GO:0005516 - calmodulin binding
GO:0005524 - ATP binding
GO:0005634 - nucleus

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

290348

KS6A4
Ribosomal protein S6 kinase alpha-4

4

15

High-throughput

0.903

High

461922

HS90A
Heat shock protein HSP 90-alpha

4

20

Small-scale

0.881

High

468791

HS90B
Heat shock protein HSP 90-beta

3

20

High-throughput

0.817

High

256681

HNRC1
Heterogeneous nuclear ribonucleoprotein C-like 1

2

5

High-throughput

0.737

High

259098

DNJA2
DnaJ homolog subfamily A member 2

2

7

High-throughput

0.737

High

462632

HNRPC
Heterogeneous nuclear ribonucleoproteins C1/C2

2

4

High-throughput

0.737

High

485409

ROA1
Heterogeneous nuclear ribonucleoprotein A1

2

10

High-throughput

0.737

High

727959

DNJA1
DnaJ homolog subfamily A member 1

2

7

High-throughput

0.737

High

909692

ROA3
Heterogeneous nuclear ribonucleoprotein A3

2

10

High-throughput

0.737

High

1148475

SAFB1
Scaffold attachment factor B1

2

5

High-throughput

0.737

High

1153021

MYLK
Myosin light chain kinase, smooth muscle

2

16

High-throughput

0.737

High

1158532

CDC37
Hsp90 co-chaperone Cdc37

2

8

High-throughput

0.737

High

1181542

RA1L2
Heterogeneous nuclear ribonucleoprotein A1-like 2

2

10

High-throughput

0.737

High

1431203

RALY
RNA-binding protein Raly

2

3

High-throughput

0.737

High

396922

ESR1
Estrogen receptor

5

16

High-throughput

0.700

High

1293815

FBX11
F-box only protein 11

4

1

High-throughput

0.699

High

564352

CFTR
Cystic fibrosis transmembrane conductance regulator

1

1

High-throughput

0.698

High

443513

CDK1
Cyclin-dependent kinase 1

1

9

High-throughput

0.691

High

1025591

CDK16
Cyclin-dependent kinase 16

1

16

High-throughput

0.691

High

1054143

BTK
Tyrosine-protein kinase BTK

1

11

Small-scale

0.691

High

691422

1433T
14-3-3 protein theta

3

20

High-throughput

0.633

High

721481

GSTT1
Glutathione S-transferase theta-1

2

1

High-throughput

0.628

High

990720

1433Z
14-3-3 protein zeta/delta

3

20

High-throughput

0.624

High

242887

PRKN
E3 ubiquitin-protein ligase parkin

1

14

High-throughput

0.618

High

458479

ADRB2
Beta-2 adrenergic receptor

1

2

High-throughput

0.618

High

1026775

NFKB2
Nuclear factor NF-kappa-B p100 subunit

1

10

High-throughput

0.618

High

1097163

MK07
Mitogen-activated protein kinase 7

1

20

Small-scale

0.618

High

1098843

M3K1
Mitogen-activated protein kinase kinase kinase 1

1

19

High-throughput

0.618

High

1407239

M3K3
Mitogen-activated protein kinase kinase kinase 3

1

16

High-throughput

0.618

High

1431194

MYLK2
Myosin light chain kinase 2, skeletal/cardiac muscle

1

17

High-throughput

0.618

High

1367244

ESR2
Estrogen receptor beta

2

16

High-throughput

0.601

High

1202029

H90B3
Putative heat shock protein HSP 90-beta-3

1

20

High-throughput

0.575

High

141101

NOP56
Nucleolar protein 56

2

5

High-throughput

0.571

High

243733

SMCA5
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5

2

3

High-throughput

0.571

High

415856

HSPB1
Heat shock protein beta-1

2

12

High-throughput

0.571

High

419200

H2A1B
Histone H2A type 1-B/E

2

10

High-throughput

0.571

High

502123

H2AZ
Histone H2A.Z

2

14

High-throughput

0.571

High

537891

HSP7C
Heat shock cognate 71 kDa protein

2

5

High-throughput

0.571

High

542217

TOP1
DNA topoisomerase 1

2

1

High-throughput

0.571

High

616364

RCC1
Regulator of chromosome condensation

2

3

High-throughput

0.571

High

761870

HSP74
Heat shock 70 kDa protein 4

2

8

High-throughput

0.571

High

778780

PRS7
26S proteasome regulatory subunit 7

2

10

High-throughput

0.571

High

916345

TXTP
Tricarboxylate transport protein, mitochondrial

2

1

High-throughput

0.571

High

936853

NP1L1
Nucleosome assembly protein 1-like 1

2

3

High-throughput

0.571

High

937340

DSRAD
Double-stranded RNA-specific adenosine deaminase

2

3

High-throughput

0.571

High

966863

1433G
14-3-3 protein gamma

2

20

High-throughput

0.571

High

968806

PRS4
26S proteasome regulatory subunit 4

2

8

High-throughput

0.571

High

980309

H4
Histone H4

2

3

High-throughput

0.571

High

998795

H31
Histone H3.1

2

15

High-throughput

0.571

High

1017202

H33
Histone H3.3

2

15

High-throughput

0.571

High

1162042

H31T
Histone H3.1t

2

15

High-throughput

0.571

High

1239479

H3C
Histone H3.3C

2

15

High-throughput

0.571

High

1258939

H32
Histone H3.2

2

15

High-throughput

0.571

High

1259639

H2AV
Histone H2A.V

2

14

High-throughput

0.571

High

1272822

H2A3
Histone H2A type 3

2

10

High-throughput

0.571

High

1372247

H2A1C
Histone H2A type 1-C

2

10

High-throughput

0.571

High

1404387

CDC5L
Cell division cycle 5-like protein

2

2

High-throughput

0.571

High

1406923

NP1L4
Nucleosome assembly protein 1-like 4

2

3

High-throughput

0.571

High

1431198

DDX18
ATP-dependent RNA helicase DDX18

2

2

High-throughput

0.571

High

1419216

TRI54
Tripartite motif-containing protein 54

1

18

High-throughput

0.568

High

211045

PSMD3
26S proteasome non-ATPase regulatory subunit 3

2

0

High-throughput

0.547

High

425029

ADT2
ADP/ATP translocase 2

2

0

High-throughput

0.547

High

1323153

CCAR2
Cell cycle and apoptosis regulator protein 2

2

0

High-throughput

0.547

High

1429611

NAT10
RNA cytidine acetyltransferase

2

0

High-throughput

0.547

High

1431200

TECR
Very-long-chain enoyl-CoA reductase

2

0

High-throughput

0.547

High

227967

MYO1B
Unconventional myosin-Ib

1

14

High-throughput

0.536

High

509966

TRI49
Tripartite motif-containing protein 49

1

4

High-throughput

0.536

High

667446

RPB1
DNA-directed RNA polymerase II subunit RPB1

1

2

High-throughput

0.536

High

723085

RPB2
DNA-directed RNA polymerase II subunit RPB2

1

1

High-throughput

0.536

High

992956

RACK1
Small ribosomal subunit protein RACK1

1

6

High-throughput

0.536

High

1055034

MEF2C
Myocyte-specific enhancer factor 2C

1

3

Small-scale

0.536

High

1116450

FHL1
Four and a half LIM domains protein 1

1

8

High-throughput

0.536

High

1403723

YMEL1
ATP-dependent zinc metalloprotease YME1L1

1

1

High-throughput

0.536

High

1431202

TES
Testin

1

5

High-throughput

0.536

High

347316

BAG2
BAG family molecular chaperone regulator 2

2

3

High-throughput

0.521

High

389763

FETUA
Alpha-2-HS-glycoprotein

2

2

High-throughput

0.521

High

1042070

LMNB2
Lamin-B2

2

11

High-throughput

0.521

High

1141416

WDR43
WD repeat-containing protein 43

2

5

High-throughput

0.521

High

1314239

SPB1
pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3

2

5

High-throughput

0.521

High

1351469

BRX1
Ribosome biogenesis protein BRX1 homolog

2

1

High-throughput

0.521

High

1431205

NOP58
Nucleolar protein 58

2

5

High-throughput

0.521

High

327647

ELP1
Elongator complex protein 1

1

2

Small-scale

0.515

High

301693

WFS1
Wolframin

1

2

High-throughput

0.515

High

842239

THIM
3-ketoacyl-CoA thiolase, mitochondrial

1

2

High-throughput

0.489

High

791714

SYUA
Alpha-synuclein

1

3

High-throughput

0.481

High

278157

FLNB
Filamin-B

1

20

High-throughput

0.479

High

637727

FLNA
Filamin-A

1

20

High-throughput

0.479

High

648228

NDKB
Nucleoside diphosphate kinase B

1

1

High-throughput

0.479

High

730882

1433S
14-3-3 protein sigma

1

20

High-throughput

0.479

High

772416

PCKGC
Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

1

1

Small-scale

0.479

High

848301

MAGA3
Melanoma-associated antigen 3

1

6

Small-scale

0.479

High

971989

1433E
14-3-3 protein epsilon

1

9

High-throughput

0.479

High

1090911

TRAF2
TNF receptor-associated factor 2

1

7

High-throughput

0.479

High

1341021

THOC2
THO complex subunit 2

1

11

High-throughput

0.479

High

1380848

TRI63
E3 ubiquitin-protein ligase TRIM63

1

18

High-throughput

0.479

High

1403340

RUFY1
RUN and FYVE domain-containing protein 1

1

10

High-throughput

0.479

High

1419288

TRI55
Tripartite motif-containing protein 55

1

19

High-throughput

0.479

High

1431197

SMUF2
E3 ubiquitin-protein ligase SMURF2

1

13

Small-scale

0.479

High

913264

TF2AA
Transcription initiation factor IIA subunit 1

1

0

High-throughput

0.458

High

1181575

Q32Q12
Nucleoside diphosphate kinase

1

1

High-throughput

0.445

High

630357

TNR1B
Tumor necrosis factor receptor superfamily member 1B

1

4

High-throughput

0.437

High

1431204

RTRAF
RNA transcription, translation and transport factor protein

1

0

Small-scale

0.426

High

942354

BACE1
Beta-secretase 1

1

2

Small-scale

0.407

High

1431199

TM260
Protein O-mannosyl-transferase TMEM260

3

0

High-throughput

0.347

High

1431195

MTRFR
Mitochondrial translation release factor in rescue

3

0

High-throughput

0.342

High

1431196

EMRE
Essential MCU regulator, mitochondrial

3

0

High-throughput

0.342

High

139606

PESC
Pescadillo homolog

2

0

High-throughput

0.298

High

462805

QCR6
Cytochrome b-c1 complex subunit 6, mitochondrial

2

0

High-throughput

0.298

High

1140021

RRS1
Ribosome biogenesis regulatory protein homolog

2

0

High-throughput

0.298

High

1407701

EBP2
Probable rRNA-processing protein EBP2

2

0

High-throughput

0.298

High

1431201

QCR9
Cytochrome b-c1 complex subunit 9

2

0

High-throughput

0.298

High

402412

GBA1
Lysosomal acid glucosylceramidase

1

0

Small-scale

0.249

Low

1431206

CIA30
Complex I intermediate-associated protein 30, mitochondrial

1

0

Small-scale

0.249

Low


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Last updated: 11Aug2025