Protein: ENO1
Enolase 1 (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase 1) (2-phosphoglycerate dehydratase 1)

Gene: ENO1
Organism: Saccharomyces cerevisiae S288C
Interactions: 208
UniProt ID: P00924

Gene Ontology (top 5):
GO:0000015 - phosphopyruvate hydratase complex
GO:0000287 - magnesium ion binding
GO:0000324 - fungal-type vacuole
GO:0004634 - phosphopyruvate hydratase activity
GO:0005737 - cytoplasm

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

367631

ENO1
Enolase 1

7

13

Small-scale

0.952

High

367632

ENO2
Enolase 2

2

13

High-throughput

0.754

High

367665

SSB1
Ribosome-associated molecular chaperone SSB1

3

7

High-throughput

0.650

High

367774

RPN11
Ubiquitin carboxyl-terminal hydrolase RPN11

3

3

High-throughput

0.640

High

367675

HSC82
ATP-dependent molecular chaperone HSC82

3

8

High-throughput

0.633

High

367661

HSP71
Heat shock protein SSA1

3

7

High-throughput

0.624

High

367658

HSP77
Import motor subunit, mitochondrial

2

4

High-throughput

0.611

High

367699

KPC1
Protein kinase C-like 1

2

3

High-throughput

0.601

High

359095

G3P3
Glyceraldehyde-3-phosphate dehydrogenase 3

2

9

Small-scale

0.584

High

367750

RPN10
26S proteasome regulatory subunit RPN10

2

1

Small-scale

0.584

High

367635

HSP82
ATP-dependent molecular chaperone HSP82

2

8

High-throughput

0.571

High

367662

HSP72
Heat shock protein SSA2

2

7

High-throughput

0.552

High

367721

HSP7F
Heat shock protein homolog SSE1

2

10

High-throughput

0.552

High

367761

SSB2
Ribosome-associated molecular chaperone SSB2

2

7

High-throughput

0.552

High

367651

LTE1
Guanine nucleotide exchange factor LTE1

1

2

High-throughput

0.536

High

367722

PP12
Serine/threonine-protein phosphatase PP1-2

1

2

High-throughput

0.536

High

367767

MAD2
Mitotic spindle checkpoint component MAD2

1

1

High-throughput

0.536

High

367797

UBP3
Ubiquitin carboxyl-terminal hydrolase 3

1

4

High-throughput

0.536

High

367754

TCPZ
T-complex protein 1 subunit zeta

2

1

High-throughput

0.521

High

367765

DSN1
Kinetochore-associated protein DSN1

2

1

High-throughput

0.521

High

367701

SIS1
Protein SIS1

1

2

High-throughput

0.515

High

367645

KAPB
cAMP-dependent protein kinase type 2

1

3

High-throughput

0.500

High

367652

PBS2
MAP kinase kinase PBS2

1

5

High-throughput

0.500

High

367653

FUMH
Fumarate hydratase, mitochondrial

1

3

High-throughput

0.500

High

367668

EI2BD
Translation initiation factor eIF2B subunit delta

1

2

High-throughput

0.500

High

367670

EI2BA
Translation initiation factor eIF2B subunit alpha

1

2

High-throughput

0.500

High

367682

PHO85
Cyclin-dependent protein kinase PHO85

1

5

High-throughput

0.500

High

367685

GBB
Guanine nucleotide-binding protein subunit beta

1

4

High-throughput

0.500

High

367687

CSK22
Casein kinase II subunit alpha'

1

4

High-throughput

0.500

High

367690

FKBP
FK506-binding protein 1

1

4

High-throughput

0.500

High

367692

PP11
Serine/threonine-protein phosphatase PP1-1

1

1

High-throughput

0.500

High

367693

PWP1
Periodic tryptophan protein 1

1

5

High-throughput

0.500

High

367700

MEK1
Meiosis-specific serine/threonine-protein kinase MEK1

1

5

High-throughput

0.500

High

367706

GID7
GID complex subunit 7

1

2

High-throughput

0.500

High

367708

PPZ1
Serine/threonine-protein phosphatase PP-Z1

1

2

High-throughput

0.500

High

367723

FIMB
Fimbrin

1

2

High-throughput

0.500

High

367734

VPS21
Vacuolar protein sorting-associated protein 21

1

1

High-throughput

0.500

High

367740

IMB2
Importin subunit beta-2

1

1

High-throughput

0.500

High

367759

LIS1
Nuclear distribution protein PAC1

1

4

High-throughput

0.500

High

367766

SA185
SIT4-associating protein SAP185

1

1

High-throughput

0.500

High

367773

RIM15
Serine/threonine-protein kinase RIM15

1

5

High-throughput

0.500

High

367775

SA155
SIT4-associating protein SAP155

1

1

High-throughput

0.500

High

367787

SSK2
MAP kinase kinase kinase SSK2

1

2

High-throughput

0.500

High

367794

RHO4
GTP-binding protein RHO4

1

1

High-throughput

0.500

High

367804

STE20
Serine/threonine-protein kinase STE20

1

3

High-throughput

0.500

High

367816

IMH1
Golgin IMH1

1

8

High-throughput

0.500

High

367831

AP2M
AP-2 complex subunit mu

1

1

High-throughput

0.500

High

367681

SPO12
Sporulation-specific protein 12

1

1

High-throughput

0.489

High

367664

ARF1
ADP-ribosylation factor 1

1

0

High-throughput

0.479

High

367672

AMPL
Vacuolar aminopeptidase 1

1

0

High-throughput

0.479

High

367702

RV161
Reduced viability upon starvation protein 161

1

0

High-throughput

0.479

High

367720

IF2G
Eukaryotic translation initiation factor 2 subunit gamma

1

0

High-throughput

0.479

High

367728

CBF5
H/ACA ribonucleoprotein complex subunit CBF5

1

0

High-throughput

0.479

High

367757

SLN1
Osmosensing histidine protein kinase SLN1

1

0

High-throughput

0.479

High

367768

PAC11
WD repeat-containing protein PAC11

1

0

High-throughput

0.479

High

367795

2ABA
Protein phosphatase PP2A regulatory subunit B

1

0

High-throughput

0.479

High

367801

UME1
Transcriptional regulatory protein UME1

1

0

High-throughput

0.479

High

367818

PSR2
Probable phosphatase PSR2

1

0

High-throughput

0.479

High

367826

GET3
ATPase GET3

1

0

High-throughput

0.479

High

358918

G3P2
Glyceraldehyde-3-phosphate dehydrogenase 2

1

9

High-throughput

0.479

High

359389

G3P1
Glyceraldehyde-3-phosphate dehydrogenase 1

1

9

High-throughput

0.479

High

361398

SODC
Superoxide dismutase [Cu-Zn]

1

5

High-throughput

0.479

High

364845

KPYK1
Pyruvate kinase 1

1

4

High-throughput

0.479

High

365506

PGK
Phosphoglycerate kinase

1

3

High-throughput

0.479

High

367633

TPIS
Triosephosphate isomerase

1

4

High-throughput

0.479

High

367634

PMG1
Phosphoglycerate mutase 1

1

3

High-throughput

0.479

High

367636

RL25
Large ribosomal subunit protein uL23

1

2

High-throughput

0.479

High

367637

HXKA
Hexokinase-1

1

5

High-throughput

0.479

High

367638

HXKB
Hexokinase-2

1

5

High-throughput

0.479

High

367639

VDAC1
Non-selective voltage-gated ion channel 1

1

1

Small-scale

0.479

High

367640

METE
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

1

1

High-throughput

0.479

High

367641

RL26A
Large ribosomal subunit protein uL24A

1

1

High-throughput

0.479

High

367642

RS3
Small ribosomal subunit protein uS3

1

1

High-throughput

0.479

High

367643

SC160
Protein SCP160

1

3

High-throughput

0.479

High

367644

PDC1
Pyruvate decarboxylase isozyme 1

1

1

High-throughput

0.479

High

367646

RS14A
Small ribosomal subunit protein uS11A

1

1

High-throughput

0.479

High

367647

KAD2
Adenylate kinase

1

1

High-throughput

0.479

High

367648

DHE4
NADP-specific glutamate dehydrogenase 1

1

1

High-throughput

0.479

High

367655

SYIC
Isoleucine--tRNA ligase, cytoplasmic

1

2

High-throughput

0.479

High

367656

RS22A
Small ribosomal subunit protein uS8A

1

1

High-throughput

0.479

High

367659

RS8A
Small ribosomal subunit protein eS8A

1

1

High-throughput

0.479

High

367660

RS18A
Small ribosomal subunit protein uS13A

1

1

High-throughput

0.479

High

367667

G6PI
Glucose-6-phosphate isomerase

1

1

High-throughput

0.479

High

367669

ALF
Fructose-bisphosphate aldolase

1

1

High-throughput

0.479

High

367671

CYPH
Peptidyl-prolyl cis-trans isomerase

1

3

High-throughput

0.479

High

367674

TAL1
Transaldolase

1

1

High-throughput

0.479

High

367676

STI1
Heat shock protein STI1

1

6

High-throughput

0.479

High

367678

THRC
Threonine synthase

1

2

High-throughput

0.479

High

367686

PGTB1
Geranylgeranyl transferase type-1 subunit beta

1

1

High-throughput

0.479

High

367694

XRN1
5'-3' exoribonuclease 1

1

1

High-throughput

0.479

High

367696

HSP12
12 kDa heat shock protein

1

1

High-throughput

0.479

High

367697

TKT1
Transketolase 1

1

3

High-throughput

0.479

High

367703

RS2
Small ribosomal subunit protein uS5

1

3

High-throughput

0.479

High

367707

CDC48
Cell division control protein 48

1

4

High-throughput

0.479

High

367709

RS7A
Small ribosomal subunit protein eS7A

1

2

High-throughput

0.479

High

367713

DHOM
Homoserine dehydrogenase

1

1

High-throughput

0.479

High

367714

SYKM
Lysine--tRNA ligase, mitochondrial

1

4

Small-scale

0.479

High

367715

P5CR
Pyrroline-5-carboxylate reductase

1

2

High-throughput

0.479

High

367726

SMY2
Protein SMY2

1

1

High-throughput

0.479

High

367729

HSP78
Heat shock protein 78, mitochondrial

1

1

High-throughput

0.479

High

367731

RS3A1
Small ribosomal subunit protein eS1A

1

1

High-throughput

0.479

High

367732

TCTP
Translationally-controlled tumor protein homolog

1

2

High-throughput

0.479

High

367733

RS27A
Small ribosomal subunit protein eS27A

1

1

High-throughput

0.479

High

367739

ATG8
Autophagy-related protein 8

1

8

High-throughput

0.479

High

367742

AIM4
Altered inheritance of mitochondria protein 4

1

1

High-throughput

0.479

High

367747

RS20
Small ribosomal subunit protein uS10

1

2

High-throughput

0.479

High

367748

6PGD1
6-phosphogluconate dehydrogenase, decarboxylating 1

1

2

High-throughput

0.479

High

367749

RPN1
26S proteasome regulatory subunit RPN1

1

2

High-throughput

0.479

High

367751

CH10
10 kDa heat shock protein, mitochondrial

1

5

High-throughput

0.479

High

367756

LAA1
AP-1 accessory protein LAA1

1

1

High-throughput

0.479

High

367758

RSP5
E3 ubiquitin-protein ligase RSP5

1

7

High-throughput

0.479

High

367763

MLP2
Protein MLP2

1

10

High-throughput

0.479

High

367770

MMM1
Maintenance of mitochondrial morphology protein 1

1

1

High-throughput

0.479

High

367777

DUG1
Cys-Gly metallodipeptidase DUG1

1

2

High-throughput

0.479

High

367778

MPS3
Spindle pole body assembly component MPS3

1

1

High-throughput

0.479

High

367782

RS12
Small ribosomal subunit protein eS12

1

1

High-throughput

0.479

High

367786

PIL1
Sphingolipid long chain base-responsive protein PIL1

1

1

High-throughput

0.479

High

367789

GIS2
Zinc finger protein GIS2

1

3

High-throughput

0.479

High

367791

SAM50
Sorting assembly machinery 50 kDa subunit

1

1

High-throughput

0.479

High

367796

NUM1
Nuclear migration protein NUM1

1

9

High-throughput

0.479

High

367798

RS15
Small ribosomal subunit protein uS19

1

2

High-throughput

0.479

High

367802

COFI
Cofilin

1

3

High-throughput

0.479

High

367803

TOM1
E3 ubiquitin-protein ligase TOM1

1

7

High-throughput

0.479

High

367805

OYE2
NADPH dehydrogenase 2

1

1

High-throughput

0.479

High

367812

PUR8
Adenylosuccinate lyase

1

3

High-throughput

0.479

High

367815

IRC20
Uncharacterized ATP-dependent helicase IRC20

1

2

Small-scale

0.479

High

367819

ZEO1
Protein ZEO1

1

1

High-throughput

0.479

High

367820

SGO1
Shugoshin

1

1

High-throughput

0.479

High

367821

MCA1
Metacaspase-1

1

1

High-throughput

0.479

High

367823

TMA46
Translation machinery-associated protein 46

1

2

High-throughput

0.479

High

367824

MDN1
Midasin

1

7

High-throughput

0.479

High

367830

SMC6
Structural maintenance of chromosomes protein 6

1

8

High-throughput

0.479

High

367663

RT01
Small ribosomal subunit protein mS43

1

0

High-throughput

0.458

High

367666

ERF1
Eukaryotic peptide chain release factor subunit 1

1

0

High-throughput

0.458

High

367684

ADT2
ADP,ATP carrier protein 2

1

0

Small-scale

0.458

High

367695

RPC2
DNA-directed RNA polymerase III subunit RPC2

1

0

High-throughput

0.458

High

367716

RPC3
DNA-directed RNA polymerase III subunit RPC3

1

0

High-throughput

0.458

High

367718

RM41
Large ribosomal subunit protein uL23m

1

0

High-throughput

0.458

High

367725

GSP1
GTP-binding nuclear protein GSP1/CNR1

1

0

High-throughput

0.458

High

367772

MID1
Stretch-activated cation channel MID1

1

0

Small-scale

0.458

High

367680

TUP1
General transcriptional corepressor TUP1

1

3

High-throughput

0.457

High

367688

PRP6
Pre-mRNA-splicing factor 6

1

2

High-throughput

0.457

High

367689

PRP4
U4/U6 small nuclear ribonucleoprotein PRP4

1

2

High-throughput

0.457

High

367704

DMC1
Meiotic recombination protein DMC1

1

1

High-throughput

0.457

High

367717

ARP2
Actin-related protein 2

1

2

High-throughput

0.457

High

367719

CDC3
Cell division control protein 3

1

1

High-throughput

0.457

High

367735

SWD2
COMPASS component SWD2

1

3

High-throughput

0.457

High

367738

AP3M
AP-3 complex subunit mu

1

1

High-throughput

0.457

High

367741

SIF2
SIR4-interacting protein SIF2

1

4

High-throughput

0.457

High

367744

ARPC1
Actin-related protein 2/3 complex subunit 1

1

4

High-throughput

0.457

High

367746

RCK1
Serine/threonine-protein kinase RCK1

1

4

High-throughput

0.457

High

367769

BUB1
Spindle assembly checkpoint serine/threonine-protein kinase BUB1

1

5

High-throughput

0.457

High

367771

COPB2
Coatomer subunit beta'

1

3

High-throughput

0.457

High

367784

SEH1
Nucleoporin SEH1

1

5

High-throughput

0.457

High

367788

COPA
Coatomer subunit alpha

1

3

High-throughput

0.457

High

367806

WDR59
Maintenance of telomere capping protein 5

1

5

High-throughput

0.457

High

367807

UTP5
U3 small nucleolar RNA-associated protein 5

1

3

High-throughput

0.457

High

367811

ERB1
Ribosome biogenesis protein ERB1

1

3

High-throughput

0.457

High

367654

HSP73
Heat shock protein SSA3

1

7

High-throughput

0.445

High

367657

UBI4P
Polyubiquitin [Cleaved into: Ubiquitin]

1

4

High-throughput

0.445

High

367673

SEC63
Protein translocation protein SEC63

1

3

High-throughput

0.445

High

367679

BIP
Endoplasmic reticulum chaperone BiP

1

1

High-throughput

0.445

High

367691

SRP54
Signal recognition particle subunit SRP54

1

1

High-throughput

0.445

High

367705

MAS5
Mitochondrial protein import protein MAS5

1

1

High-throughput

0.445

High

367743

CLPX
ATP-dependent clpX-like chaperone, mitochondrial

1

3

High-throughput

0.445

High

367755

CAJ1
Protein CAJ1

1

2

High-throughput

0.445

High

367760

HSP7E
Heat shock protein SSC3, mitochondrial

1

4

High-throughput

0.445

High

367780

TCPQ
T-complex protein 1 subunit theta

1

2

High-throughput

0.445

High

367783

CWC23
Pre-mRNA-splicing factor CWC23

1

2

High-throughput

0.445

High

367800

ULP1
Ubiquitin-like-specific protease 1

1

4

High-throughput

0.439

High

367710

PEX3
Peroxisomal biogenesis factor 3

1

1

High-throughput

0.437

High

367711

TF2B
Transcription initiation factor IIB

1

4

High-throughput

0.437

High

367712

TF3B
Transcription factor IIIB 70 kDa subunit

1

4

High-throughput

0.437

High

367730

IXR1
Intrastrand cross-link recognition protein

1

3

Small-scale

0.437

High

367737

ANM1
Protein arginine N-methyltransferase 1

1

1

High-throughput

0.437

High

367745

YGP1
Protein YGP1

1

1

High-throughput

0.437

High

367764

ULP2
Ubiquitin-like-specific protease 2

1

4

Small-scale

0.437

High

367781

MOG1
Nuclear import protein MOG1

1

1

High-throughput

0.437

High

367792

ACT
Actin

1

1

Small-scale

0.437

High

367799

MLP1
Protein MLP1

1

10

High-throughput

0.437

High

367814

URN1
Pre-mRNA-splicing factor URN1

1

1

High-throughput

0.437

High

367822

AP3D
AP-3 complex subunit delta

1

3

High-throughput

0.437

High

367827

SMT3
Ubiquitin-like protein SMT3

1

4

High-throughput

0.437

High

367829

DBP10
ATP-dependent RNA helicase DBP10

1

5

High-throughput

0.437

High

367677

HSP26
Heat shock protein 26

1

0

High-throughput

0.426

High

367736

KAE1
tRNA N6-adenosine threonylcarbamoyltransferase

1

0

High-throughput

0.426

High

367785

TAM41
Phosphatidate cytidylyltransferase, mitochondrial

1

0

High-throughput

0.426

High

367809

HSP31
Glutathione-independent glyoxalase HSP31

1

0

High-throughput

0.426

High

367752

TCPG
T-complex protein 1 subunit gamma

1

1

High-throughput

0.407

High

367753

TCPD
T-complex protein 1 subunit delta

1

3

High-throughput

0.407

High

367776

ERJ5
ER-localized J domain-containing protein 5

1

1

High-throughput

0.407

High

367649

ARD1
N-terminal acetyltransferase A complex catalytic subunit ARD1

2

0

High-throughput

0.313

High

367650

RPAC1
DNA-directed RNA polymerases I and III subunit RPAC1

1

0

High-throughput

0.261

Low

367698

VAN1
Mannan polymerase I complex VAN1 subunit

1

0

High-throughput

0.261

Low

367724

CDC13
Cell division control protein 13

1

0

High-throughput

0.261

Low

367727

SUI1
Eukaryotic translation initiation factor eIF-1

1

0

High-throughput

0.261

Low

367793

YE066
Putative uncharacterized protein YER066C-A

1

0

High-throughput

0.261

Low

367810

SPC24
Kinetochore protein SPC24

1

0

High-throughput

0.261

Low

367817

PRP11
Pre-mRNA-splicing factor PRP11

1

0

High-throughput

0.261

Low

367828

MED4
Mediator of RNA polymerase II transcription subunit 4

1

0

High-throughput

0.261

Low

367683

TPM1
Tropomyosin-1

1

0

Small-scale

0.249

Low

367813

MMS22
E3 ubiquitin-protein ligase substrate receptor MMS22

1

0

High-throughput

0.249

Low

367762

PSF2
DNA replication complex GINS protein PSF2

1

0

High-throughput

0.232

Low

367779

NOP9
Nucleolar protein 9

1

0

High-throughput

0.232

Low

367790

NAF1
H/ACA ribonucleoprotein complex non-core subunit NAF1

1

0

High-throughput

0.232

Low

367808

BUD22
Bud site selection protein 22

1

0

High-throughput

0.232

Low

367825

NSL1
Kinetochore-associated protein NSL1

1

0

High-throughput

0.232

Low


Service provided by Combinatics, Japan

Last updated: 11Aug2025