Protein: NDC80
Kinetochore protein NDC80 (80 kDa spindle component protein) (Nuclear division cycle protein 80) (Two-hybrid interaction with DMC1 protein 3)

Gene: NDC80
Organism: Saccharomyces cerevisiae S288C
Interactions: 120
UniProt ID: P40460

Gene Ontology (top 5):
GO:0000776 - kinetochore
GO:0000779 - condensed chromosome, centromeric region
GO:0000940 - outer kinetochore
GO:0005634 - nucleus
GO:0007059 - chromosome segregation

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

755249

NUF2
Kinetochore protein NUF2

28

18

Small-scale

0.993

High

824871

SPC24
Kinetochore protein SPC24

27

0

Small-scale

0.941

High

824840

NDC80
Kinetochore protein NDC80

2

17

Small-scale

0.781

High

824862

MPS1
Serine/threonine-protein kinase MPS1

7

20

Small-scale

0.750

High

816990

SPC25
Kinetochore protein SPC25

14

0

Small-scale

0.730

High

824841

DSN1
Kinetochore-associated protein DSN1

16

0

Small-scale

0.729

High

824857

DAM1
DASH complex subunit DAM1

11

0

Small-scale

0.725

High

824844

STU2
Protein STU2

4

9

Small-scale

0.696

High

814074

MTW1
Kinetochore-associated protein MTW1

4

1

Small-scale

0.689

High

781903

SPC34
DASH complex subunit SPC34

6

0

Small-scale

0.686

High

808635

AURK
Aurora kinase

3

20

Small-scale

0.667

High

776694

ASK1
DASH complex subunit ASK1

4

0

Small-scale

0.648

High

824854

KNL1
Outer kinetochore KNL1 complex subunit SPC105

2

1

Small-scale

0.635

High

824842

CENPT
Inner kinetochore subunit CNN1

3

0

Small-scale

0.634

High

524155

HSP71
Heat shock protein SSA1

3

18

Small-scale

0.633

High

526860

HSP72
Heat shock protein SSA2

3

18

Small-scale

0.624

High

824847

NNF1
Kinetochore-associated protein NNF1

3

0

High-throughput

0.621

High

824869

SPC19
DASH complex subunit SPC19

3

0

Small-scale

0.614

High

486206

TBA1
Tubulin alpha-1 chain

2

10

Small-scale

0.585

High

824886

DAD1
DASH complex subunit DAD1

2

0

Small-scale

0.576

High

808592

SMC2
Structural maintenance of chromosomes protein 2

1

12

Small-scale

0.573

High

670794

SIS1
Protein SIS1

2

5

Small-scale

0.571

High

741705

HSP7F
Heat shock protein homolog SSE1

2

20

Small-scale

0.571

High

824870

ZWINT
Outer kinetochore KNL1 complex subunit KRE28

2

0

High-throughput

0.570

High

824868

FIN1
Filament protein FIN1

2

0

Small-scale

0.561

High

646050

KIN3
Serine/threonine-protein kinase KIN3

1

20

Small-scale

0.561

High

797884

CENPU
Inner kinetochore subunit AME1

2

0

Small-scale

0.559

High

824881

SGO1
Shugoshin

2

0

Small-scale

0.559

High

824865

HSK3
DASH complex subunit HSK3

2

0

Small-scale

0.552

High

753099

NIP80
Protein NIP100

2

18

High-throughput

0.543

High

744865

SLA1
Actin cytoskeleton-regulatory complex protein SLA1

1

6

High-throughput

0.541

High

694214

BUD14
Bud site selection protein 14

1

0

High-throughput

0.536

High

824845

BBC1
Myosin tail region-interacting protein MTI1

1

15

High-throughput

0.512

High

824883

MSH5
MutS protein homolog 5

1

1

High-throughput

0.508

High

699412

CIN8
Kinesin-like protein CIN8

1

20

Small-scale

0.501

High

824846

PTK2
Serine/threonine-protein kinase PTK2/STK2

1

20

High-throughput

0.501

High

792411

BOI1
BEM1-interacting protein 1

1

11

High-throughput

0.494

High

803360

HSE1
Class E vacuolar protein-sorting machinery protein HSE1

1

20

High-throughput

0.494

High

805400

BZZ1
Protein BZZ1

1

10

High-throughput

0.494

High

816029

BOI2
BEM1-interacting protein 2

1

12

High-throughput

0.494

High

824872

NKP2
Inner kinetochore subunit NKP2

1

0

High-throughput

0.493

High

603487

VATA
V-type proton ATPase catalytic subunit A

1

7

High-throughput

0.489

High

824859

ARPC2
Actin-related protein 2/3 complex subunit 2

1

1

High-throughput

0.489

High

824873

YL456
Pyridoxamine 5'-phosphate oxidase YLR456W homolog

1

0

High-throughput

0.486

High

824874

ATG17
Autophagy-related protein 17

1

0

High-throughput

0.486

High

824882

CUB1

1

0

High-throughput

0.486

High

824890

MED4
Mediator of RNA polymerase II transcription subunit 4

1

0

High-throughput

0.486

High

675291

DMC1
Meiotic recombination protein DMC1

1

3

Small-scale

0.481

High

735263

KNS1
Dual specificity protein kinase KNS1

1

15

High-throughput

0.481

High

751267

CNS1
Hsp70/Hsp90 co-chaperone CNS1

1

12

Small-scale

0.481

High

824850

UBC9
SUMO-conjugating enzyme UBC9

1

2

High-throughput

0.481

High

824853

MCM6
DNA replication licensing factor MCM6

1

14

Small-scale

0.481

High

824879

ADY3
Accumulates dyads protein 3

1

19

High-throughput

0.481

High

824885

SIZ2
E3 SUMO-protein ligase SIZ2

1

3

High-throughput

0.481

High

824888

SMT3
Ubiquitin-like protein SMT3

1

1

High-throughput

0.481

High

594406

RPB3
DNA-directed RNA polymerase II subunit RPB3

1

2

High-throughput

0.479

High

707285

TF2B
Transcription initiation factor IIB

1

2

High-throughput

0.479

High

735509

IRE1
Serine/threonine-protein kinase/endoribonuclease IRE1

1

13

High-throughput

0.479

High

739974

CDC5
Cell cycle serine/threonine-protein kinase CDC5/MSD2

1

17

Small-scale

0.479

High

740945

PUB1
Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1

1

10

High-throughput

0.479

High

743229

PP12
Serine/threonine-protein phosphatase PP1-2

1

16

Small-scale

0.479

High

824884

RIO1
Serine/threonine-protein kinase RIO1

1

2

High-throughput

0.479

High

792723

YPK3
Serine/threonine-protein kinase YPK3

1

16

High-throughput

0.470

High

824891

YD199
Putative metabolite transport protein YDL199C

1

1

High-throughput

0.470

High

654725

SPA2
Protein SPA2

1

10

High-throughput

0.464

High

734518

MSB2
Signaling mucin MSB2

1

13

High-throughput

0.464

High

737321

CDC12
Cell division control protein 12

1

8

High-throughput

0.464

High

483783

SWI1
SWI/SNF chromatin-remodeling complex subunit SWI1

1

0

High-throughput

0.460

High

740267

SMI1
Cell wall assembly regulator SMI1

1

0

Small-scale

0.460

High

745478

SLX5
E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX5

1

0

Small-scale

0.460

High

824852

UFD1
Ubiquitin fusion degradation protein 1

1

0

High-throughput

0.460

High

824860

NIS1
Protein NIS1

1

0

High-throughput

0.460

High

824892

MPC70
Sporulation-specific protein 21

1

0

High-throughput

0.460

High

824856

YBP2
YAP1-binding protein 2

1

0

Small-scale

0.458

High

824867

CENPP
Inner kinetochore subunit CTF19

1

0

Small-scale

0.458

High

824875

CENPO
Inner kinetochore subunit MCM21

1

0

Small-scale

0.458

High

824889

SCM3
Protein SCM3

1

0

Small-scale

0.458

High

481642

HSP73
Heat shock protein SSA3

1

18

High-throughput

0.445

High

545123

SSB1
Ribosome-associated molecular chaperone SSB1

1

18

High-throughput

0.445

High

736014

SP110
Spindle pole body component 110

1

19

Small-scale

0.445

High

818927

SSB2
Ribosome-associated molecular chaperone SSB2

1

18

High-throughput

0.445

High

824851

PFD6
Prefoldin subunit 6

1

1

High-throughput

0.445

High

779765

DYHC
Dynein heavy chain, cytoplasmic

1

12

High-throughput

0.439

High

809190

END3
Actin cytoskeleton-regulatory complex protein END3

1

18

High-throughput

0.439

High

824849

KEL2
Kelch repeat-containing protein 2

1

17

High-throughput

0.439

High

738157

NGG1
SAGA complex subunit NGG1

1

0

High-throughput

0.438

High

744611

TFB1
General transcription and DNA repair factor IIH subunit TFB1

1

0

High-throughput

0.438

High

624762

PMA2
Plasma membrane ATPase 2

1

3

High-throughput

0.437

High

810824

TCPG
T-complex protein 1 subunit gamma

1

5

High-throughput

0.437

High

824880

UBR2
E3 ubiquitin-protein ligase UBR2

1

2

Small-scale

0.437

High

782576

DAD2
DASH complex subunit DAD2

1

0

Small-scale

0.426

High

797109

CENPL
Inner kinetochore subunit IML3

1

0

High-throughput

0.426

High

824855

DUO1
DASH complex subunit DUO1

1

0

Small-scale

0.426

High

824863

DAD3
DASH complex subunit DAD3

1

0

Small-scale

0.426

High

824864

DAD4
DASH complex subunit DAD4

1

0

Small-scale

0.426

High

824887

CENPH
Inner kinetochore subunit MCM16

1

0

High-throughput

0.426

High

824893

CENPI
Inner kinetochore subunit CTF3

1

0

High-throughput

0.426

High

824894

CENPW
Inner kinetochore subunit WIP1

1

0

High-throughput

0.426

High

386735

TBB
Tubulin beta chain

1

7

Small-scale

0.407

High

394623

EF1A
Elongation factor 1-alpha

1

5

High-throughput

0.407

High

752336

HSP78
Heat shock protein 78, mitochondrial

1

2

High-throughput

0.407

High

816238

HSP7E
Heat shock protein SSC3, mitochondrial

1

20

High-throughput

0.407

High

824848

HLJ1
Protein HLJ1

1

9

High-throughput

0.407

High

824876

SWA2
Auxilin-like clathrin uncoating factor SWA2

1

11

High-throughput

0.407

High

796319

FL2D
Protein MUM2

2

0

High-throughput

0.310

High

818109

SHO1
High osmolarity signaling protein SHO1

1

0

High-throughput

0.282

High

824866

PEX13
Peroxisomal membrane protein PEX13

1

0

High-throughput

0.282

High

757659

IF4B
Eukaryotic translation initiation factor 4B

1

0

High-throughput

0.279

Low

797213

SHE3
SWI5-dependent HO expression protein 3

1

0

High-throughput

0.279

Low

824861

VAC7
Vacuolar segregation protein 7

1

0

High-throughput

0.269

Low

824858

COG2
Conserved oligomeric Golgi complex subunit 2

1

0

High-throughput

0.265

Low

600690

SEC2
Rab guanine nucleotide exchange factor SEC2

1

0

High-throughput

0.251

Low

782940

JNM1
Nuclear migration protein JNM1

1

0

High-throughput

0.251

Low

790326

VPP2
V-type proton ATPase subunit a, Golgi isoform

1

0

High-throughput

0.251

Low

816686

VAB2
Biogenesis of lysosome-related organelles complex 1 subunit VAB2

1

0

High-throughput

0.251

Low

824895

HPH1
Protein HPH1

1

0

High-throughput

0.251

Low

824877

FMP45
SUR7 family protein FMP45

1

0

High-throughput

0.249

Low

824878

HBT1
Protein HBT1

1

0

High-throughput

0.249

Low

824843

FAR3
Factor arrest protein 3

1

0

Small-scale

0.238

Low


Service provided by Combinatics, Japan

Last updated: 11Aug2025