Protein: HSP74
Heat shock protein SSA4

Gene: HSP74, SSA4
Organism: Saccharomyces cerevisiae S288C
Interactions: 155
UniProt ID: P22202

Gene Ontology (top 5):
GO:0005524 - ATP binding
GO:0005634 - nucleus
GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0005886 - plasma membrane

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

645683

SIS1
Protein SIS1

4

20

Small-scale

0.881

High

525938

HSP72
Heat shock protein SSA2

3

16

High-throughput

0.839

High

392239

HSP82
ATP-dependent molecular chaperone HSP82

3

19

Small-scale

0.827

High

645686

MAS5
Mitochondrial protein import protein MAS5

3

20

Small-scale

0.827

High

578860

HSC82
ATP-dependent molecular chaperone HSC82

3

19

High-throughput

0.827

High

523076

HSP71
Heat shock protein SSA1

2

16

High-throughput

0.789

High

645702

HSP7F
Heat shock protein homolog SSE1

2

17

High-throughput

0.789

High

481483

HSP73
Heat shock protein SSA3

2

16

High-throughput

0.783

High

585587

STI1
Heat shock protein STI1

2

20

Small-scale

0.757

High

645764

APJ1
J domain-containing protein APJ1

2

20

Small-scale

0.754

High

645703

HSP79
Heat shock protein homolog SSE2

2

17

High-throughput

0.737

High

543834

SSB1
Ribosome-associated molecular chaperone SSB1

1

19

High-throughput

0.691

High

645734

CSK2C
Casein kinase II subunit beta'

2

4

High-throughput

0.621

High

645769

MCE1
mRNA-capping enzyme subunit alpha

2

2

High-throughput

0.621

High

510201

HSP77
Import motor subunit, mitochondrial

1

17

High-throughput

0.618

High

644711

CLH
Clathrin heavy chain

2

7

High-throughput

0.611

High

645679

HSP74
Heat shock protein SSA4

1

15

High-throughput

0.590

High

645726

FES1
Hsp70 nucleotide exchange factor FES1

2

9

Small-scale

0.586

High

513327

RL23B
Large ribosomal subunit protein uL14B

2

1

High-throughput

0.584

High

645696

HS104
Heat shock protein 104

2

10

Small-scale

0.584

High

645722

GBLP
Small ribosomal subunit protein RACK1

2

18

Small-scale

0.584

High

645748

RPN11
Ubiquitin carboxyl-terminal hydrolase RPN11

2

10

High-throughput

0.584

High

597039

BIP
Endoplasmic reticulum chaperone BiP

1

12

High-throughput

0.575

High

645713

MDJ1
DnaJ homolog 1, mitochondrial

1

20

High-throughput

0.575

High

645738

HSP7E
Heat shock protein SSC3, mitochondrial

1

16

High-throughput

0.575

High

645708

HSP78
Heat shock protein 78, mitochondrial

2

11

High-throughput

0.571

High

645757

MSN5
Protein MSN5

1

12

Small-scale

0.561

High

359148

G3P3
Glyceraldehyde-3-phosphate dehydrogenase 3

1

10

High-throughput

0.536

High

645739

IMB4
Importin subunit beta-4

1

11

High-throughput

0.536

High

645777

ISW2
ISWI chromatin-remodeling complex ATPase ISW2

1

20

High-throughput

0.536

High

561246

CG13
G1/S-specific cyclin CLN3

1

9

High-throughput

0.515

High

645690

PCL2
PHO85 cyclin-2

1

5

High-throughput

0.508

High

645712

CLG1
PHO85 cyclin CLG1

1

3

High-throughput

0.508

High

645778

PCL8
PHO85 cyclin-8

1

8

High-throughput

0.508

High

645704

GSP1
GTP-binding nuclear protein GSP1/CNR1

1

12

Small-scale

0.501

High

459022

RPAC1
DNA-directed RNA polymerases I and III subunit RPAC1

1

4

High-throughput

0.500

High

628038

FKBP
FK506-binding protein 1

1

15

High-throughput

0.500

High

645707

CBF5
H/ACA ribonucleoprotein complex subunit CBF5

1

2

High-throughput

0.500

High

645735

TCPB
T-complex protein 1 subunit beta

1

13

High-throughput

0.500

High

645749

ATG18
Autophagy-related protein 18

1

14

High-throughput

0.500

High

645766

2ABA
Protein phosphatase PP2A regulatory subunit B

1

5

High-throughput

0.500

High

645768

AP1M1
AP-1 complex subunit mu-1-I

1

16

High-throughput

0.500

High

645790

AP2M
AP-2 complex subunit mu

1

17

High-throughput

0.500

High

645743

LYS12
Homoisocitrate dehydrogenase, mitochondrial

1

6

High-throughput

0.489

High

645758

UTP8
U3 small nucleolar RNA-associated protein 8

1

2

High-throughput

0.489

High

638702

RIR1
Ribonucleoside-diphosphate reductase large chain 1

1

3

Small-scale

0.481

High

149588

RS9A
Small ribosomal subunit protein uS4A

1

4

High-throughput

0.479

High

364930

KPYK1
Pyruvate kinase 1

1

5

High-throughput

0.479

High

368408

TPIS
Triosephosphate isomerase

1

2

High-throughput

0.479

High

368591

PMG1
Phosphoglycerate mutase 1

1

9

High-throughput

0.479

High

383523

RS17A
Small ribosomal subunit protein eS17A

1

2

High-throughput

0.479

High

402192

RPC1
DNA-directed RNA polymerase III subunit RPC1

1

7

Small-scale

0.479

High

409941

RL25
Large ribosomal subunit protein uL23

1

2

High-throughput

0.479

High

430781

ERF3
Eukaryotic peptide chain release factor GTP-binding subunit

1

6

Small-scale

0.479

High

433453

RL26A
Large ribosomal subunit protein uL24A

1

3

High-throughput

0.479

High

434833

RS3
Small ribosomal subunit protein uS3

1

3

High-throughput

0.479

High

435779

RS13
Small ribosomal subunit protein uS15

1

2

High-throughput

0.479

High

438617

SC160
Protein SCP160

1

11

High-throughput

0.479

High

441710

RS14A
Small ribosomal subunit protein uS11A

1

4

High-throughput

0.479

High

450520

FAS1
Fatty acid synthase subunit beta

1

6

High-throughput

0.479

High

452816

ATPA
ATP synthase subunit alpha, mitochondrial

1

7

High-throughput

0.479

High

504043

RL31A
Large ribosomal subunit protein eL31A

1

3

High-throughput

0.479

High

512262

RS6A
Small ribosomal subunit protein eS6A

1

3

High-throughput

0.479

High

512825

RS8A
Small ribosomal subunit protein eS8A

1

3

High-throughput

0.479

High

531245

METK1
S-adenosylmethionine synthase 1

1

6

High-throughput

0.479

High

534045

HSF
Heat shock transcription factor

1

18

High-throughput

0.479

High

618546

FAS2
Fatty acid synthase subunit alpha

1

3

High-throughput

0.479

High

645121

PSB4
Proteasome subunit beta type-4

1

13

Small-scale

0.479

High

645680

KCC2
Calcium/calmodulin-dependent protein kinase II

1

5

High-throughput

0.479

High

645681

CG22
G2/mitotic-specific cyclin-2

1

8

Small-scale

0.479

High

645684

RNQ1
[PIN+] prion protein RNQ1

1

1

High-throughput

0.479

High

645685

RS2
Small ribosomal subunit protein uS5

1

6

Small-scale

0.479

High

645687

MET14
Karyogamy protein KAR4

1

4

Small-scale

0.479

High

645688

TAH1
TPR repeat-containing protein associated with Hsp90

1

20

Small-scale

0.479

High

645689

PAT1
Deadenylation-dependent mRNA-decapping factor PAT1

1

6

Small-scale

0.479

High

645691

CDC48
Cell division control protein 48

1

11

High-throughput

0.479

High

645692

CYPC
Peptidyl-prolyl cis-trans isomerase C, mitochondrial

1

10

High-throughput

0.479

High

645693

RL16A
Large ribosomal subunit protein uL13A

1

3

High-throughput

0.479

High

645695

TF2B
Transcription initiation factor IIB

1

5

High-throughput

0.479

High

645697

GLNA
Glutamine synthetase

1

2

High-throughput

0.479

High

645698

EF2
Elongation factor 2

1

12

High-throughput

0.479

High

645700

RPC3
DNA-directed RNA polymerase III subunit RPC3

1

2

High-throughput

0.479

High

645701

PUB1
Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1

1

5

High-throughput

0.479

High

645705

RSSA1
Small ribosomal subunit protein uS2A

1

5

High-throughput

0.479

High

645706

SMY2
Protein SMY2

1

2

High-throughput

0.479

High

645709

IXR1
Intrastrand cross-link recognition protein

1

12

Small-scale

0.479

High

645710

RS3A1
Small ribosomal subunit protein eS1A

1

3

High-throughput

0.479

High

645711

TSA1
Peroxiredoxin TSA1

1

8

High-throughput

0.479

High

645714

DEF1
RNA polymerase II degradation factor 1

1

6

High-throughput

0.479

High

645715

RS27A
Small ribosomal subunit protein eS27A

1

4

High-throughput

0.479

High

645716

EF1G2
Elongation factor 1-gamma 2

1

8

High-throughput

0.479

High

645720

GLYC
Serine hydroxymethyltransferase, cytosolic

1

4

High-throughput

0.479

High

645721

GLY1
Low specificity L-threonine aldolase

1

2

High-throughput

0.479

High

645723

AHP1
Peroxiredoxin AHP1

1

2

High-throughput

0.479

High

645724

ATG8
Autophagy-related protein 8

1

19

High-throughput

0.479

High

645725

APN2
DNA-(apurinic or apyrimidinic site) endonuclease 2

1

4

Small-scale

0.479

High

645727

GID4
GID complex substrate-recognition subunit 4

1

2

High-throughput

0.479

High

645729

RS20
Small ribosomal subunit protein uS10

1

3

High-throughput

0.479

High

645730

NMD3
60S ribosomal export protein NMD3

1

2

Small-scale

0.479

High

645742

TM108
Protein TMA108

1

9

High-throughput

0.479

High

645744

DSN1
Kinetochore-associated protein DSN1

1

3

High-throughput

0.479

High

645745

MKT1
Post-transcriptional regulator MKT1

1

2

High-throughput

0.479

High

645747

PXA1
Peroxisomal long-chain fatty acid import protein 2

1

5

High-throughput

0.479

High

645750

NMD5
Nonsense-mediated mRNA decay protein 5

1

13

Small-scale

0.479

High

645753

RS12
Small ribosomal subunit protein eS12

1

3

High-throughput

0.479

High

645754

VATG
V-type proton ATPase subunit G

1

4

High-throughput

0.479

High

645756

SKP1
Suppressor of kinetochore protein 1

1

3

Small-scale

0.479

High

645762

IGO1
mRNA stability protein IGO1

1

2

Small-scale

0.479

High

645763

NAF1
H/ACA ribonucleoprotein complex non-core subunit NAF1

1

3

Small-scale

0.479

High

645765

ALDH6
Magnesium-activated aldehyde dehydrogenase, cytosolic

1

9

High-throughput

0.479

High

645767

TPS1
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit

1

8

High-throughput

0.479

High

645770

RS15
Small ribosomal subunit protein uS19

1

3

High-throughput

0.479

High

645771

TOM1
E3 ubiquitin-protein ligase TOM1

1

11

High-throughput

0.479

High

645773

BUL2
Ubiquitin ligase-binding protein BUL2

1

5

High-throughput

0.479

High

645775

PDS5
Sister chromatid cohesion protein PDS5

1

4

High-throughput

0.479

High

645776

TFC6
Transcription factor tau 91 kDa subunit

1

2

High-throughput

0.479

High

645779

TMA46
Translation machinery-associated protein 46

1

2

High-throughput

0.479

High

645780

YL11B
Transposon Ty1-LR1 Gag-Pol polyprotein

1

10

High-throughput

0.479

High

645781

SGT2
Small glutamine-rich tetratricopeptide repeat-containing protein 2

1

20

Small-scale

0.479

High

645784

HSP42
Heat shock protein 42

1

4

High-throughput

0.479

High

645785

DBP10
ATP-dependent RNA helicase DBP10

1

12

High-throughput

0.479

High

645788

CMR1
DNA damage-binding protein CMR1

1

12

High-throughput

0.479

High

645789

SMC6
Structural maintenance of chromosomes protein 6

1

11

High-throughput

0.479

High

561703

HAP3
Transcriptional activator HAP3

1

5

High-throughput

0.457

High

645732

AIM46
Altered inheritance of mitochondria protein 46, mitochondrial

1

3

High-throughput

0.457

High

645746

COPB2
Coatomer subunit beta'

1

11

High-throughput

0.457

High

645759

COPA
Coatomer subunit alpha

1

14

High-throughput

0.457

High

645774

WDR59
Maintenance of telomere capping protein 5

1

20

High-throughput

0.457

High

509401

UBI4P
Polyubiquitin [Cleaved into: Ubiquitin]

1

12

High-throughput

0.445

High

626589

HSP60
Heat shock protein 60, mitochondrial

1

7

High-throughput

0.445

High

645728

CLPX
ATP-dependent clpX-like chaperone, mitochondrial

1

7

High-throughput

0.445

High

645731

RIX1
Pre-rRNA-processing protein RIX1

1

2

High-throughput

0.445

High

645733

FKBP3
Peptidyl-prolyl cis-trans isomerase

1

16

High-throughput

0.445

High

645736

TCPD
T-complex protein 1 subunit delta

1

12

High-throughput

0.445

High

645741

6P21
6-phosphofructo-2-kinase 1

1

11

High-throughput

0.445

High

645752

CYP7
Peptidyl-prolyl cis-trans isomerase CYP7

1

15

Small-scale

0.445

High

645760

LTO1
Protein LTO1

1

2

High-throughput

0.445

High

645772

GCN5
Histone acetyltransferase GCN5

1

10

High-throughput

0.445

High

645782

NSL1
Kinetochore-associated protein NSL1

1

2

High-throughput

0.445

High

382832

RMAR
Small ribosomal subunit protein uS3m

1

2

High-throughput

0.437

High

645682

ERG6
Sterol 24-C-methyltransferase ERG6

1

5

High-throughput

0.437

High

645694

COQ3
Ubiquinone biosynthesis O-methyltransferase, mitochondrial

1

3

Small-scale

0.437

High

645699

OAC1
Mitochondrial oxaloacetate transport protein

1

15

High-throughput

0.437

High

645717

MCR1
NADH-cytochrome b5 reductase 2

1

10

Small-scale

0.437

High

645718

PSG1
PMA1 stabilization in the Golgi protein 1 [Cleaved into: PSG1-N'; PSG1-C']

1

2

High-throughput

0.437

High

645719

MIC60
MICOS complex subunit MIC60

1

2

High-throughput

0.437

High

645755

TOM22
Mitochondrial import receptor subunit TOM22

1

3

High-throughput

0.437

High

645783

PER33
Pore and endoplasmic reticulum protein of 33 kDa

1

4

High-throughput

0.437

High

645786

YOP1
Protein YOP1

1

3

High-throughput

0.437

High

645740

PSF2
DNA replication complex GINS protein PSF2

1

0

High-throughput

0.426

High

645761

CWC25
Pre-mRNA-splicing factor CWC25

1

1

High-throughput

0.407

High

645787

POM34
Nucleoporin POM34

1

0

Small-scale

0.296

High

645737

MBO1
Gamma tubulin complex adapter SPC72

1

0

High-throughput

0.251

Low

645751

GWT1
GPI-anchored wall transfer protein 1

1

0

High-throughput

0.232

Low


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Last updated: 11Aug2025