Protein: SPB1
27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase (EC 2.1.1.167) (2'-O-ribose RNA methyltransferase SPB1) (AdoMet-dependent rRNA methyltransferase SPB1) (S-adenosyl-L-methionine-dependent methyltransferase) (Suppressor of PAB1 protein 1)

Gene: SPB1
Organism: Saccharomyces cerevisiae S288C
Interactions: 131
UniProt ID: P25582

Gene Ontology (top 5):
GO:0000463 - maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0000466 - maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0005634 - nucleus
GO:0005730 - nucleolus
GO:0008650 - rRNA (uridine-2'-O-)-methyltransferase activity

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

677237

RRP17
Ribosomal RNA-processing protein 17

4

1

High-throughput

0.886

High

677245

NOC3
Nucleolar complex-associated protein 3

4

3

High-throughput

0.873

High

677258

YTM1
Ribosome biogenesis protein YTM1

3

10

High-throughput

0.827

High

409959

RL25
Large ribosomal subunit protein uL23

3

3

Small-scale

0.817

High

677168

SPB1
27S pre-rRNA

2

9

High-throughput

0.767

High

677261

MAK21
Ribosome biogenesis protein MAK21

2

4

High-throughput

0.767

High

677265

IF6
Eukaryotic translation initiation factor 6

2

1

Small-scale

0.754

High

677187

RPF2
Ribosome biogenesis protein RPF2

7

2

High-throughput

0.745

High

677221

PESC
Pescadillo homolog

7

2

Small-scale

0.743

High

677240

ERB1
Ribosome biogenesis protein ERB1

2

6

High-throughput

0.737

High

677246

RLP24
Ribosome biogenesis protein RLP24

2

3

High-throughput

0.737

High

677259

NOP53
Ribosome biogenesis protein NOP53

6

3

Small-scale

0.730

High

677205

NUG1
Nuclear GTP-binding protein NUG1

6

7

Small-scale

0.728

High

677263

DBP10
ATP-dependent RNA helicase DBP10

5

7

High-throughput

0.713

High

677170

SPB4
ATP-dependent rRNA helicase SPB4

5

11

High-throughput

0.709

High

677203

NOC2
Nucleolar complex protein 2

5

1

Small-scale

0.704

High

677220

NSA1
Ribosome biogenesis protein NSA1

5

1

Small-scale

0.702

High

677224

NOP15
Ribosome biogenesis protein 15

4

6

High-throughput

0.691

High

677213

LOC1
60S ribosomal subunit assembly/export protein LOC1

5

0

High-throughput

0.688

High

677177

PP12
Serine/threonine-protein phosphatase PP1-2

3

1

High-throughput

0.667

High

677185

EBP2
rRNA-processing protein EBP2

3

1

High-throughput

0.646

High

677199

RPF1
Ribosome production factor 1

3

1

High-throughput

0.646

High

677201

FKBP3
Peptidyl-prolyl cis-trans isomerase

3

2

High-throughput

0.646

High

677241

FKBP4
FK506-binding protein 4

3

2

High-throughput

0.646

High

677248

NOP12
Nucleolar protein 12

3

7

High-throughput

0.646

High

677196

CIC1
Proteasome-interacting protein CIC1

3

3

High-throughput

0.640

High

677211

NOP2
25S rRNA

3

5

Small-scale

0.640

High

677232

NOG1
Nucleolar GTP-binding protein 1

3

5

Small-scale

0.640

High

677234

HAS1
ATP-dependent RNA helicase HAS1

3

12

High-throughput

0.640

High

677186

RRP14
Ribosomal RNA-processing protein 14

3

0

High-throughput

0.618

High

633134

MAK11
Protein MAK11

1

8

High-throughput

0.618

High

673546

NLE1
Ribosome assembly protein 4

1

6

High-throughput

0.618

High

677189

GBLP
Small ribosomal subunit protein RACK1

1

7

High-throughput

0.618

High

677238

RRB1
Ribosome assembly protein RRB1

1

6

High-throughput

0.618

High

677244

NOC4
Nucleolar complex protein 4

1

4

High-throughput

0.618

High

677223

NOP13
Nucleolar protein 13

2

3

High-throughput

0.611

High

677264

NOP58
Nucleolar protein 58

2

4

Small-scale

0.601

High

419619

H2A2
Histone H2A.2

2

5

High-throughput

0.584

High

677197

SSF1
Ribosome biogenesis protein SSF1

2

2

High-throughput

0.584

High

677200

RIX1
Pre-rRNA-processing protein RIX1

2

1

Small-scale

0.584

High

677210

RLP7
Ribosome biogenesis protein RLP7

2

4

High-throughput

0.584

High

677222

NOG2
Nucleolar GTP-binding protein 2

2

7

High-throughput

0.584

High

677235

ARX1
Probable metalloprotease ARX1

2

2

High-throughput

0.584

High

531620

RL4A
Large ribosomal subunit protein uL4A

1

2

High-throughput

0.575

High

677215

URB2
Nucleolar pre-ribosomal-associated protein 2

2

0

High-throughput

0.569

High

677219

PRP43
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43

1

4

High-throughput

0.536

High

585214

FBRL
rRNA 2'-O-methyltransferase fibrillarin

1

1

Small-scale

0.534

High

569918

RL3
Large ribosomal subunit protein uL3

2

3

High-throughput

0.533

High

656936

BUR1
Serine/threonine-protein kinase BUR1

1

3

High-throughput

0.515

High

677251

DIA2
Protein DIA2

1

0

High-throughput

0.513

High

656705

KC12
Casein kinase I homolog 2

1

1

High-throughput

0.508

High

403374

PABP
Polyadenylate-binding protein, cytoplasmic and nuclear

1

4

Small-scale

0.501

High

677216

CLA4
Serine/threonine-protein kinase CLA4

1

4

Small-scale

0.501

High

677233

STE20
Serine/threonine-protein kinase STE20

1

5

Small-scale

0.501

High

677252

ESA1
Histone acetyltransferase ESA1

1

2

Small-scale

0.501

High

149612

RS9A
Small ribosomal subunit protein uS4A

1

3

High-throughput

0.479

High

380985

H2B1
Histone H2B.1

1

6

High-throughput

0.479

High

381422

H2B2
Histone H2B.2

1

6

High-throughput

0.479

High

432502

RL7A
Large ribosomal subunit protein uL30A

1

4

High-throughput

0.479

High

434863

RS3
Small ribosomal subunit protein uS3

1

1

High-throughput

0.479

High

435802

RS13
Small ribosomal subunit protein uS15

1

2

High-throughput

0.479

High

512285

RS6A
Small ribosomal subunit protein eS6A

1

1

High-throughput

0.479

High

534083

MAK16
Protein MAK16

1

2

High-throughput

0.479

High

670388

SIS1
Protein SIS1

1

1

High-throughput

0.479

High

674444

RS2
Small ribosomal subunit protein uS5

1

3

High-throughput

0.479

High

677169

CDC48
Cell division control protein 48

1

3

High-throughput

0.479

High

677171

RL5
Large ribosomal subunit protein uL18

1

2

Small-scale

0.479

High

677172

RL16A
Large ribosomal subunit protein uL13A

1

3

High-throughput

0.479

High

677173

RS7A
Small ribosomal subunit protein eS7A

1

2

High-throughput

0.479

High

677174

TF2B
Transcription initiation factor IIB

1

2

High-throughput

0.479

High

677176

RPC3
DNA-directed RNA polymerase III subunit RPC3

1

1

High-throughput

0.479

High

677178

GSP1
GTP-binding nuclear protein GSP1/CNR1

1

4

High-throughput

0.479

High

677179

DRS1
ATP-dependent RNA helicase DRS1

1

10

High-throughput

0.479

High

677181

MRT4
Ribosome assembly factor MRT4

1

2

High-throughput

0.479

High

677184

RRP1
Ribosomal RNA-processing protein 1

1

3

High-throughput

0.479

High

677188

NOP4
Nucleolar protein 4

1

5

High-throughput

0.479

High

677191

MAK5
ATP-dependent RNA helicase MAK5

1

11

High-throughput

0.479

High

677193

YBC8
UPF0642 protein YBL028C

1

1

High-throughput

0.479

High

677194

ENP1
Essential nuclear protein 1

1

2

High-throughput

0.479

High

677198

IPI1
Pre-rRNA-processing protein IPI1

1

1

High-throughput

0.479

High

677206

NSA2
Ribosome biogenesis protein NSA2

1

2

High-throughput

0.479

High

677207

BMT5
25S rRNA

1

1

High-throughput

0.479

High

677208

ULP2
Ubiquitin-like-specific protease 2

1

2

High-throughput

0.479

High

677209

DSN1
Kinetochore-associated protein DSN1

1

2

High-throughput

0.479

High

677214

ALB1
Ribosome biogenesis protein ALB1

1

1

High-throughput

0.479

High

677226

RCM1
25S rRNA

1

5

High-throughput

0.479

High

677229

NOP3
Serine/arginine

1

11

High-throughput

0.479

High

677236

SCC2
Sister chromatid cohesion protein 2

1

1

High-throughput

0.479

High

677239

PUF6
Pumilio homology domain family member 6

1

1

High-throughput

0.479

High

677242

JIP5
WD repeat-containing protein JIP5

1

8

High-throughput

0.479

High

677247

BUD20
Bud site selection protein 20

1

1

High-throughput

0.479

High

677249

BRX1
Ribosome biogenesis protein BRX1

1

9

High-throughput

0.479

High

677255

RRS1
Regulator of ribosome biosynthesis

1

3

High-throughput

0.479

High

677256

NIP7
60S ribosome subunit biogenesis protein NIP7

1

1

High-throughput

0.479

High

677257

MDN1
Midasin

1

10

High-throughput

0.479

High

677262

RL7B
Large ribosomal subunit protein uL30B

1

4

High-throughput

0.479

High

436333

RS31
Ubiquitin-ribosomal protein eS31 fusion protein [Cleaved into: Ubiquitin; Small ribosomal subunit protein eS31

1

0

High-throughput

0.458

High

441732

RS14A
Small ribosomal subunit protein uS11A

1

0

High-throughput

0.458

High

603018

EST1
Telomere elongation protein EST1

1

0

High-throughput

0.458

High

677175

BMH1
Protein BMH1

1

0

High-throughput

0.458

High

677183

URB1
Nucleolar pre-ribosomal-associated protein 1

1

0

Small-scale

0.458

High

677204

NOP16
Nucleolar protein 16

1

0

High-throughput

0.458

High

677212

UTP10
U3 small nucleolar RNA-associated protein 10

1

0

High-throughput

0.458

High

677218

RL37A
Large ribosomal subunit protein eL37A

1

0

High-throughput

0.458

High

677225

APJ1
J domain-containing protein APJ1

1

0

High-throughput

0.458

High

677231

XRN2
5'-3' exoribonuclease 2

1

0

High-throughput

0.458

High

677243

RRP15
Ribosomal RNA-processing protein 15

1

0

High-throughput

0.458

High

677250

SGO1
Shugoshin

1

0

High-throughput

0.458

High

677260

SCC1
Sister chromatid cohesion protein 1

1

0

High-throughput

0.458

High

383542

RS17A
Small ribosomal subunit protein eS17A

1

2

High-throughput

0.437

High

433476

RL26A
Large ribosomal subunit protein uL24A

1

4

High-throughput

0.437

High

454905

RS19A
Small ribosomal subunit protein eS19A

1

2

High-throughput

0.437

High

502876

RS22A
Small ribosomal subunit protein uS8A

1

1

High-throughput

0.437

High

504061

RL31A
Large ribosomal subunit protein eL31A

1

1

High-throughput

0.437

High

512846

RS8A
Small ribosomal subunit protein eS8A

1

3

High-throughput

0.437

High

677180

RSSA1
Small ribosomal subunit protein uS2A

1

3

High-throughput

0.437

High

677182

RS3A1
Small ribosomal subunit protein eS1A

1

3

High-throughput

0.437

High

677190

RL32
Large ribosomal subunit protein eL32

1

1

High-throughput

0.437

High

677202

LAA1
AP-1 accessory protein LAA1

1

1

High-throughput

0.437

High

677228

NUM1
Nuclear migration protein NUM1

1

5

High-throughput

0.437

High

677230

RS15
Small ribosomal subunit protein uS19

1

1

High-throughput

0.437

High

677254

RS10A
Small ribosomal subunit protein eS10A

1

3

High-throughput

0.437

High

677227

RL34A
Large ribosomal subunit protein eL34A

1

0

High-throughput

0.426

High

569721

RL30
Large ribosomal subunit protein eL30

1

2

High-throughput

0.407

High

677253

HRK1
Serine/threonine-protein kinase HRK1

1

0

High-throughput

0.279

Low

94539

STE2
Pheromone alpha factor receptor

1

0

High-throughput

0.251

Low

514482

RS18A
Small ribosomal subunit protein uS13A

1

0

High-throughput

0.249

Low

677192

NU170
Nucleoporin NUP170

1

0

High-throughput

0.249

Low

677195

RS20
Small ribosomal subunit protein uS10

1

0

High-throughput

0.249

Low

677217

RS12
Small ribosomal subunit protein eS12

1

0

High-throughput

0.249

Low


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Last updated: 11Aug2025