Protein: GPN1
GPN-loop GTPase 1 (EC 3.6.5.-) (Essential PCL1-interacting ATPase 1) (GPN-loop GTPase NPA3) (Nucleolar preribosomal-associated protein 3)

Gene: GPN1, NPA3
Organism: Saccharomyces cerevisiae S288C
Interactions: 153
UniProt ID: P47122

Gene Ontology (top 5):
GO:0003924 - GTPase activity
GO:0005525 - GTP binding
GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0006606 - protein import into nucleus

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

865653

GPN2
GPN-loop GTPase 2

7

10

Small-scale

0.959

High

865648

GPN3
GPN-loop GTPase 3

5

10

Small-scale

0.936

High

865623

GPN1
GPN-loop GTPase 1

2

10

Small-scale

0.737

High

818388

RTR1
RNA polymerase II subunit B1 CTD phosphatase RTR1

5

3

Small-scale

0.713

High

470553

RPB2
DNA-directed RNA polymerase II subunit RPB2

10

13

Small-scale

0.685

High

594449

RPB3
DNA-directed RNA polymerase II subunit RPB3

7

8

Small-scale

0.672

High

402046

RPB1
DNA-directed RNA polymerase II subunit RPB1

7

9

Small-scale

0.669

High

579675

HSC82
ATP-dependent molecular chaperone HSC82

3

3

High-throughput

0.640

High

806936

RPB11
DNA-directed RNA polymerase II subunit RPB11

4

7

Small-scale

0.612

High

722064

XPO1
Exportin-1

2

13

Small-scale

0.611

High

865644

RBA50
RNA polymerase II-associated protein RBA50

2

3

Small-scale

0.595

High

632184

RPAB1
DNA-directed RNA polymerases I, II, and III subunit RPABC1

3

3

Small-scale

0.585

High

392983

HSP82
ATP-dependent molecular chaperone HSP82

2

3

Small-scale

0.584

High

850079

RPN11
Ubiquitin carboxyl-terminal hydrolase RPN11

2

1

High-throughput

0.584

High

647158

RPC2
DNA-directed RNA polymerase III subunit RPC2

3

13

Small-scale

0.581

High

632358

RPAB3
DNA-directed RNA polymerases I, II, and III subunit RPABC3

3

3

Small-scale

0.576

High

527073

HSP72
Heat shock protein SSA2

2

4

Small-scale

0.571

High

545423

SSB1
Ribosome-associated molecular chaperone SSB1

2

5

Small-scale

0.571

High

626799

HSP60
Heat shock protein 60, mitochondrial

2

1

Small-scale

0.571

High

803603

RPN1
26S proteasome regulatory subunit RPN1

2

1

High-throughput

0.571

High

667039

PCL1
PHO85 cyclin-1

1

0

Small-scale

0.561

High

633484

PP11
Serine/threonine-protein phosphatase PP1-1

1

1

High-throughput

0.536

High

645074

RPAB5
DNA-directed RNA polymerases I, II, and III subunit RPABC5

2

4

High-throughput

0.533

High

699963

RPB9
DNA-directed RNA polymerase II subunit RPB9

2

5

Small-scale

0.514

High

441381

KAPB
cAMP-dependent protein kinase type 2

1

0

High-throughput

0.513

High

574709

KSS1
Mitogen-activated protein kinase KSS1

1

0

High-throughput

0.513

High

575356

EI2BA
Translation initiation factor eIF2B subunit alpha

1

0

High-throughput

0.513

High

738066

MKK2
MAP kinase kinase MKK2/SSP33

1

0

High-throughput

0.513

High

865630

BUD32
EKC/KEOPS complex subunit BUD32

1

0

High-throughput

0.513

High

865645

KU80
ATP-dependent DNA helicase II subunit 2

1

0

High-throughput

0.513

High

793947

TAF5
SAGA complex/transcription factor TFIID complex subunit TAF5

1

1

High-throughput

0.489

High

602542

PHO85
Cyclin-dependent protein kinase PHO85

1

0

High-throughput

0.480

High

656530

KC11
Casein kinase I homolog 1

1

0

High-throughput

0.480

High

666201

MEK1
Meiosis-specific serine/threonine-protein kinase MEK1

1

0

High-throughput

0.480

High

692395

KCC1
Calcium/calmodulin-dependent protein kinase I

1

0

High-throughput

0.480

High

865626

ATG1
Serine/threonine-protein kinase ATG1

1

0

High-throughput

0.480

High

435203

RS3
Small ribosomal subunit protein uS3

1

1

High-throughput

0.479

High

455145

RS19A
Small ribosomal subunit protein eS19A

1

1

High-throughput

0.479

High

510621

HSP77
Import motor subunit, mitochondrial

1

2

Small-scale

0.479

High

518326

IF4A
ATP-dependent RNA helicase eIF4A

1

1

High-throughput

0.479

High

524431

HSP71
Heat shock protein SSA1

1

4

Small-scale

0.479

High

632275

RPAB2
DNA-directed RNA polymerases I, II, and III subunit RPABC2

1

3

High-throughput

0.479

High

727156

HS104
Heat shock protein 104

1

1

High-throughput

0.479

High

741898

HSP7F
Heat shock protein homolog SSE1

1

6

Small-scale

0.479

High

745799

GSP1
GTP-binding nuclear protein GSP1/CNR1

1

1

High-throughput

0.479

High

757336

RPB7
DNA-directed RNA polymerase II subunit RPB7

1

4

High-throughput

0.479

High

819159

SSB2
Ribosome-associated molecular chaperone SSB2

1

5

Small-scale

0.479

High

823718

RPAB4
DNA-directed RNA polymerases I, II, and III subunit RPABC4

1

5

High-throughput

0.479

High

865624

RS12
Small ribosomal subunit protein eS12

1

1

High-throughput

0.479

High

865639

RUVB1
RuvB-like protein 1

1

4

High-throughput

0.479

High

865654

RS10A
Small ribosomal subunit protein eS10A

1

1

High-throughput

0.479

High

865658

SIZ2
E3 SUMO-protein ligase SIZ2

1

1

High-throughput

0.479

High

865660

RUVB2
RuvB-like protein 2

1

4

High-throughput

0.479

High

149847

RS9A
Small ribosomal subunit protein uS4A

1

0

High-throughput

0.458

High

436045

RS13
Small ribosomal subunit protein uS15

1

0

High-throughput

0.458

High

441996

RS14A
Small ribosomal subunit protein uS11A

1

0

High-throughput

0.458

High

503129

RS22A
Small ribosomal subunit protein uS8A

1

0

High-throughput

0.458

High

504297

RL31A
Large ribosomal subunit protein eL31A

1

0

High-throughput

0.458

High

701633

PYRD
Dihydroorotate dehydrogenase

1

0

Small-scale

0.458

High

753459

RS3A1
Small ribosomal subunit protein eS1A

1

0

High-throughput

0.458

High

776607

DEF1
RNA polymerase II degradation factor 1

1

0

Small-scale

0.458

High

779494

YKT6
Synaptobrevin homolog YKT6

1

0

High-throughput

0.458

High

782535

NU133
Nucleoporin NUP133

1

0

Small-scale

0.458

High

785939

LONM
Lon protease homolog, mitochondrial

1

0

High-throughput

0.458

High

795440

ATG8
Autophagy-related protein 8

1

0

High-throughput

0.458

High

802261

RS20
Small ribosomal subunit protein uS10

1

0

High-throughput

0.458

High

825282

MOB1
DBF2 kinase activator protein MOB1

1

0

High-throughput

0.458

High

849822

BUD27
Bud site selection protein 27

1

0

High-throughput

0.458

High

863920

SET2
Histone-lysine N-methyltransferase, H3 lysine-36 specific

1

0

High-throughput

0.458

High

864768

MPS3
Spindle pole body assembly component MPS3

1

0

High-throughput

0.458

High

865631

HST2
NAD-dependent protein deacetylase HST2

1

0

High-throughput

0.458

High

865634

RS15
Small ribosomal subunit protein uS19

1

0

High-throughput

0.458

High

865637

GIS1
Transcriptional activator/repressor GIS1

1

0

High-throughput

0.458

High

865638

MTQ2
eRF1 methyltransferase catalytic subunit MTQ2

1

0

High-throughput

0.458

High

865642

DHR1
Probable ATP-dependent RNA helicase DHR1

1

0

High-throughput

0.458

High

865649

ASR1
Alcohol-sensitive RING finger protein 1

1

0

High-throughput

0.458

High

865650

SUB2
ATP-dependent RNA helicase SUB2

1

0

High-throughput

0.458

High

865651

SGO1
Shugoshin

1

0

High-throughput

0.458

High

865652

MCA1
Metacaspase-1

1

0

High-throughput

0.458

High

865657

RIO1
Serine/threonine-protein kinase RIO1

1

0

High-throughput

0.458

High

865659

DBP10
ATP-dependent RNA helicase DBP10

1

0

High-throughput

0.458

High

736174

RNP1
Ribonucleoprotein 1

1

1

High-throughput

0.457

High

751620

PPZ2
Serine/threonine-protein phosphatase PP-Z2

1

1

High-throughput

0.457

High

427346

RLA0
Large ribosomal subunit protein uL10

1

1

High-throughput

0.445

High

453673

PYR1
Multifunctional protein URA2

1

1

High-throughput

0.445

High

734144

EF2
Elongation factor 2

1

1

High-throughput

0.445

High

750970

PRS7
26S proteasome regulatory subunit 7 homolog

1

1

High-throughput

0.445

High

810722

TCPB
T-complex protein 1 subunit beta

1

2

High-throughput

0.445

High

836773

MPG1
Mannose-1-phosphate guanyltransferase

1

1

High-throughput

0.445

High

865635

PRS8
26S proteasome regulatory subunit 8 homolog

1

1

High-throughput

0.445

High

438153

NOT2
General negative regulator of transcription subunit 2

1

0

High-throughput

0.438

High

678839

AHC2
ADA histone acetyltransferase complex component 2

1

0

High-throughput

0.438

High

808224

SNF11
Transcription regulatory protein SNF11

1

0

High-throughput

0.438

High

815367

RS26A
Small ribosomal subunit protein eS26A

1

0

High-throughput

0.438

High

822398

YG5N
Uncharacterized protein YGR269W

1

0

High-throughput

0.438

High

826185

HIS4
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

1

0

High-throughput

0.438

High

849909

WWM1
WW domain-containing protein WWM1

1

0

High-throughput

0.438

High

863357

NMD5
Nonsense-mediated mRNA decay protein 5

1

0

High-throughput

0.438

High

865628

POP6
Ribonucleases P/MRP protein subunit POP6

1

0

High-throughput

0.438

High

865643

EMI2
Putative glucokinase-2

1

0

High-throughput

0.438

High

402231

RPC1
DNA-directed RNA polymerase III subunit RPC1

1

9

Small-scale

0.437

High

459125

RPAC1
DNA-directed RNA polymerases I and III subunit RPAC1

1

8

Small-scale

0.437

High

534279

RPA1
DNA-directed RNA polymerase I subunit RPA190

1

9

Small-scale

0.437

High

644951

RPA2
DNA-directed RNA polymerase I subunit RPA135

1

13

Small-scale

0.437

High

694586

SPT5
Transcription elongation factor SPT5

1

1

High-throughput

0.437

High

707532

TF2B
Transcription initiation factor IIB

1

2

High-throughput

0.437

High

865641

SIZ1
E3 SUMO-protein ligase SIZ1

1

1

High-throughput

0.437

High

386770

TBB
Tubulin beta chain

1

0

High-throughput

0.426

High

394694

EF1A
Elongation factor 1-alpha

1

0

High-throughput

0.426

High

531354

METK1
S-adenosylmethionine synthase 1

1

0

High-throughput

0.426

High

592347

VATB
V-type proton ATPase subunit B

1

0

High-throughput

0.426

High

751755

PRP8
Pre-mRNA-splicing factor 8

1

0

High-throughput

0.426

High

806758

RPN10
26S proteasome regulatory subunit RPN10

1

0

High-throughput

0.426

High

849529

GCN20
Protein GCN20

1

0

High-throughput

0.426

High

865640

RPN9
26S proteasome regulatory subunit RPN9

1

0

High-throughput

0.426

High

740187

RPN2
26S proteasome regulatory subunit RPN2

1

1

High-throughput

0.407

High

645950

DBF2
Cell cycle protein kinase DBF2

1

0

High-throughput

0.279

Low

744207

RAD24
Checkpoint protein RAD24

1

0

High-throughput

0.279

Low

605111

TPM1
Tropomyosin-1

1

0

Small-scale

0.249

Low

758229

URB1
Nucleolar pre-ribosomal-associated protein 1

1

0

Small-scale

0.249

Low

764111

SPT21
Protein SPT21

1

0

High-throughput

0.249

Low

827548

RLP7
Ribosome biogenesis protein RLP7

1

0

High-throughput

0.249

Low

836530

T2FA
Transcription initiation factor IIF subunit alpha

1

0

High-throughput

0.249

Low

836546

T2FB
Transcription initiation factor IIF subunit beta

1

0

High-throughput

0.249

Low

865632

NUM1
Nuclear migration protein NUM1

1

0

High-throughput

0.249

Low

865647

TFC6
Transcription factor tau 91 kDa subunit

1

0

High-throughput

0.249

Low

865655

AP3D
AP-3 complex subunit delta

1

0

High-throughput

0.249

Low

399854

COX4
Cytochrome c oxidase subunit 4, mitochondrial

1

0

High-throughput

0.238

Low

457155

PT494
COX3 mRNA-specific translational activator PET494

1

0

High-throughput

0.238

Low

533477

RAD18
Postreplication repair E3 ubiquitin-protein ligase RAD18

1

0

High-throughput

0.238

Low

583162

CUP2
Transcriptional activator protein CUP2

1

0

High-throughput

0.238

Low

613508

ADT3
ADP,ATP carrier protein 3

1

0

High-throughput

0.238

Low

647270

QCR9
Cytochrome b-c1 complex subunit 9, mitochondrial

1

0

High-throughput

0.238

Low

733548

MED16
Mediator of RNA polymerase II transcription subunit 16

1

0

High-throughput

0.238

Low

738159

NGG1
SAGA complex subunit NGG1

1

0

High-throughput

0.238

Low

745241

COX13
Cytochrome c oxidase subunit 13, mitochondrial

1

0

High-throughput

0.238

Low

757663

IF4B
Eukaryotic translation initiation factor 4B

1

0

High-throughput

0.238

Low

804026

MED6
Mediator of RNA polymerase II transcription subunit 6

1

0

High-throughput

0.238

Low

804046

FYV4
Small ribosomal subunit protein mS41

1

0

High-throughput

0.238

Low

805329

ERP5
Protein ERP5

1

0

High-throughput

0.238

Low

813889

GCSH
Glycine cleavage system H protein, mitochondrial

1

0

High-throughput

0.238

Low

825740

MAM33
Mitochondrial acidic protein MAM33

1

0

High-throughput

0.238

Low

826134

YIC9
Uncharacterized protein YIL029C

1

0

High-throughput

0.238

Low

849605

AAD6
Putative aryl-alcohol dehydrogenase AAD6

1

0

High-throughput

0.238

Low

849793

BST1
GPI inositol-deacylase

1

0

High-throughput

0.238

Low

865625

YGW8
Putative uncharacterized protein YGL218W

1

0

High-throughput

0.238

Low

865627

YGP2
Putative uncharacterized protein YGL152C

1

0

High-throughput

0.238

Low

865629

FHN1
Non-classical export protein 2 homolog 1

1

0

High-throughput

0.238

Low

865633

GCR2
Glycolytic genes transcriptional activator GCR2

1

0

High-throughput

0.238

Low

865636

SRL4
Oxidoreductase-like protein SRL4

1

0

High-throughput

0.238

Low

865646

ORM2
Protein ORM2

1

0

High-throughput

0.238

Low

865656

SAS10
Something about silencing protein 10

1

0

High-throughput

0.238

Low


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Last updated: 11Aug2025