Protein: PRP8
Pre-mRNA-splicing factor 8

Gene: PRP8
Organism: Saccharomyces cerevisiae S288C
Interactions: 148
UniProt ID: P33334

Gene Ontology (top 5):
GO:0000244 - spliceosomal tri-snRNP complex assembly
GO:0000350 - generation of catalytic spliceosome for second transesterification step
GO:0000387 - spliceosomal snRNP assembly
GO:0000389 - mRNA 3'-splice site recognition
GO:0000395 - mRNA 5'-splice site recognition

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

744096

BRR2
Pre-mRNA-splicing helicase BRR2

21

11

Small-scale

0.991

High

735454

AAR2
A1 cistron-splicing factor AAR2

10

2

Small-scale

0.983

High

751724

SN114
Pre-mRNA-splicing factor SNU114

10

5

Small-scale

0.976

High

751804

CWC2
Pre-mRNA-splicing factor CWC2

6

5

Small-scale

0.949

High

751781

CEF1
Pre-mRNA-splicing factor CEF1

6

3

Small-scale

0.945

High

751751

SMD3
Small nuclear ribonucleoprotein Sm D3

6

7

Small-scale

0.943

High

751744

RSMB
Small nuclear ribonucleoprotein-associated protein B

5

2

High-throughput

0.922

High

751779

PRP3
U4/U6 small nuclear ribonucleoprotein PRP3

5

1

Small-scale

0.922

High

751812

SNU66
66 kDa U4/U6.U5 small nuclear ribonucleoprotein component

5

1

Small-scale

0.922

High

751754

LSM8
LSM2-LSM8 complex subunit LSM8

5

9

Small-scale

0.916

High

751757

PRP31
Pre-mRNA-processing factor 31

4

1

High-throughput

0.896

High

624993

PRP6
Pre-mRNA-splicing factor 6

4

10

Small-scale

0.892

High

627842

PRP4
U4/U6 small nuclear ribonucleoprotein PRP4

4

4

Small-scale

0.892

High

672046

BUD31
Pre-mRNA-splicing factor BUD31

4

3

Small-scale

0.892

High

751728

LSM2
LSM complex subunit LSM2

4

7

Small-scale

0.892

High

751775

SMD1
Small nuclear ribonucleoprotein Sm D1

4

1

Small-scale

0.892

High

751771

LSM3
LSM complex subunit LSM3

4

9

Small-scale

0.875

High

751760

PRP43
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43

3

7

High-throughput

0.867

High

751764

CWC22
Pre-mRNA-splicing factor CWC22

3

1

High-throughput

0.855

High

751792

LSM6
LSM complex subunit LSM6

3

2

Small-scale

0.855

High

751746

LSM5
LSM complex subunit LSM5

4

0

Small-scale

0.850

High

751791

SMD2
Small nuclear ribonucleoprotein Sm D2

3

1

Small-scale

0.839

High

664585

PRP22
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22

3

8

Small-scale

0.834

High

751748

RUXG
Small nuclear ribonucleoprotein G

3

8

High-throughput

0.830

High

751770

RUXF
Small nuclear ribonucleoprotein F

3

2

High-throughput

0.830

High

751809

RUXE
Small nuclear ribonucleoprotein E

3

1

High-throughput

0.830

High

657960

PRP28
Pre-mRNA-splicing ATP-dependent RNA helicase PRP28

3

6

Small-scale

0.827

High

751725

NTR2
Pre-mRNA-splicing factor NTR2

3

0

Small-scale

0.796

High

700084

PRP45
Pre-mRNA-processing protein 45

2

3

High-throughput

0.789

High

739234

PRP21
Pre-mRNA-splicing factor PRP21

2

1

High-throughput

0.789

High

751745

LSM4
LSM complex subunit LSM4

2

3

High-throughput

0.789

High

751768

LSM7
LSM complex subunit LSM7

2

9

High-throughput

0.789

High

751811

PRP46
Pre-mRNA-splicing factor PRP46

2

6

High-throughput

0.789

High

702238

YJU2
Splicing factor YJU2

3

0

Small-scale

0.785

High

628199

PRP2
Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2

3

8

High-throughput

0.751

High

751766

CWC25
Pre-mRNA-splicing factor CWC25

2

0

High-throughput

0.748

High

739123

PRP19
Pre-mRNA-processing factor 19

5

7

Small-scale

0.714

High

751785

SYF1
Pre-mRNA-splicing factor SYF1

5

11

Small-scale

0.714

High

751803

RU2A
U2 small nuclear ribonucleoprotein A'

5

4

Small-scale

0.709

High

751736

LIN1
Protein LIN1

4

2

Small-scale

0.701

High

751807

CLF1
Pre-mRNA-splicing factor CLF1

4

11

Small-scale

0.691

High

751802

NCBP2
Nuclear cap-binding protein subunit 2

4

3

High-throughput

0.686

High

751774

RU17
U1 small nuclear ribonucleoprotein 70 kDa homolog

3

4

Small-scale

0.680

High

751793

SP382
Pre-mRNA-splicing factor SPP382

4

0

Small-scale

0.667

High

625083

PRP9
Pre-mRNA-splicing factor PRP9

3

1

High-throughput

0.662

High

751759

CWC23
Pre-mRNA-splicing factor CWC23

3

5

High-throughput

0.662

High

751788

RSE1
Pre-mRNA-splicing factor RSE1

3

2

High-throughput

0.662

High

751722

NCBP1
Nuclear cap-binding protein complex subunit 1

3

1

High-throughput

0.656

High

509474

UBI4P
Polyubiquitin [Cleaved into: Ubiquitin]

1

4

Small-scale

0.647

High

751780

CWC21
Pre-mRNA-splicing factor CWC21

3

0

Small-scale

0.633

High

590187

PRP16
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

1

8

High-throughput

0.618

High

638054

ISY1
Pre-mRNA-splicing factor ISY1

1

3

High-throughput

0.618

High

751750

PRP17
Pre-mRNA-processing factor 17

1

9

High-throughput

0.618

High

751777

SLU7
Pre-mRNA-splicing factor SLU7

1

1

High-throughput

0.618

High

751796

DIB1
Spliceosomal protein DIB1

1

1

High-throughput

0.618

High

751814

HUB1
Ubiquitin-like modifier HUB1

1

1

Small-scale

0.618

High

751729

SLT11
Pre-mRNA-splicing factor SLT11

2

3

High-throughput

0.611

High

751776

CUS1
Cold sensitive U2 snRNA suppressor 1

2

1

High-throughput

0.611

High

751741

PRP39
Pre-mRNA-processing factor 39

2

10

Small-scale

0.605

High

750219

PRP40
Pre-mRNA-processing protein PRP40

2

1

Small-scale

0.594

High

709664

BMH1
Protein BMH1

2

1

Small-scale

0.586

High

751752

CWC26
Pre-mRNA-splicing factor CWC26

1

0

High-throughput

0.586

High

751763

SYF2
Pre-mRNA-splicing factor SYF2

1

0

High-throughput

0.586

High

751773

PRP38
Pre-mRNA-splicing factor 38

1

0

High-throughput

0.586

High

751782

CWC15
Pre-mRNA-splicing factor CWC15

1

0

High-throughput

0.586

High

751731

SP381
Pre-mRNA-splicing factor SPP381

2

0

High-throughput

0.585

High

751789

SN309
Pre-mRNA-splicing factor SNT309

2

0

High-throughput

0.585

High

751749

DJP1
DnaJ-like protein 1

3

7

Small-scale

0.576

High

740850

PUB1
Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1

2

4

High-throughput

0.571

High

747781

SMY2
Protein SMY2

2

0

Small-scale

0.559

High

526297

HSP72
Heat shock protein SSA2

2

4

High-throughput

0.552

High

751747

SSB2
Ribosome-associated molecular chaperone SSB2

2

4

High-throughput

0.552

High

751806

UPC2
Sterol uptake control protein 2

2

0

Small-scale

0.551

High

403431

PABP
Polyadenylate-binding protein, cytoplasmic and nuclear

1

2

High-throughput

0.536

High

523495

HSP71
Heat shock protein SSA1

1

4

High-throughput

0.536

High

544327

SSB1
Ribosome-associated molecular chaperone SSB1

1

4

High-throughput

0.536

High

584355

GCN2
eIF-2-alpha kinase GCN2

1

2

High-throughput

0.536

High

751721

HSP78
Heat shock protein 78, mitochondrial

1

1

High-throughput

0.536

High

751800

LUC7
Protein LUC7

1

2

High-throughput

0.536

High

751783

SNU56
56 kDa U1 small nuclear ribonucleoprotein component

1

0

High-throughput

0.513

High

751794

URN1
Pre-mRNA-splicing factor URN1

1

0

High-throughput

0.513

High

751765

NOG2
Nucleolar GTP-binding protein 2

1

1

Small-scale

0.508

High

751723

MYO3
Myosin-3

1

2

High-throughput

0.501

High

751786

MYO5
Myosin-5

1

2

High-throughput

0.501

High

575246

RAD4
DNA repair protein RAD4

1

0

High-throughput

0.493

High

751813

YD85C
SERF-like protein YDL085C-A

1

0

High-throughput

0.493

High

751795

SWA2
Auxilin-like clathrin uncoating factor SWA2

1

3

High-throughput

0.489

High

602039

KAR3
Kinesin-like protein KAR3

1

1

Small-scale

0.481

High

697376

MIG1
Regulatory protein MIG1

1

1

Small-scale

0.481

High

751742

RSP5
E3 ubiquitin-protein ligase RSP5

1

3

Small-scale

0.481

High

381046

H2B1
Histone H2B.1

1

6

High-throughput

0.479

High

381472

H2B2
Histone H2B.2

1

6

High-throughput

0.479

High

381995

H4
Histone H4

1

1

High-throughput

0.479

High

694515

SPT5
Transcription elongation factor SPT5

1

2

Small-scale

0.479

High

751758

SF3B1
U2 snRNP component HSH155

1

2

High-throughput

0.479

High

751772

NAM8
Protein NAM8

1

2

High-throughput

0.479

High

751798

PRP11
Pre-mRNA-splicing factor PRP11

1

1

High-throughput

0.479

High

751815

HSH49
Protein HSH49

1

3

High-throughput

0.479

High

483766

SWI1
SWI/SNF chromatin-remodeling complex subunit SWI1

1

0

Small-scale

0.460

High

633218

KICH
Choline kinase

1

0

Small-scale

0.460

High

751737

DNA2
DNA replication ATP-dependent helicase/nuclease DNA2 [Includes: DNA replication nuclease DNA2

1

0

Small-scale

0.460

High

751778

ECM5
Protein ECM5

1

0

Small-scale

0.460

High

706657

TF2B
Transcription initiation factor IIB

1

0

High-throughput

0.458

High

734916

RPC3
DNA-directed RNA polymerase III subunit RPC3

1

0

High-throughput

0.458

High

738803

NAB2
Nuclear polyadenylated RNA-binding protein NAB2

1

0

High-throughput

0.458

High

743514

RU1A
U1 small nuclear ribonucleoprotein A

1

0

High-throughput

0.458

High

751726

YK21
Uncharacterized protein YKR041W

1

0

High-throughput

0.458

High

751732

NTC20
Pre-mRNA-splicing factor NTC20

1

0

High-throughput

0.458

High

751733

AIM4
Altered inheritance of mitochondria protein 4

1

0

High-throughput

0.458

High

751762

PESC
Pescadillo homolog

1

0

High-throughput

0.458

High

751784

SCC2
Sister chromatid cohesion protein 2

1

0

High-throughput

0.458

High

751790

MMS22
E3 ubiquitin-protein ligase substrate receptor MMS22

1

0

High-throughput

0.458

High

751797

RDS3
Pre-mRNA-splicing factor RDS3

1

0

High-throughput

0.458

High

751799

VAM6
Vacuolar morphogenesis protein 6

1

0

High-throughput

0.458

High

751805

RRP6
Exosome complex exonuclease RRP6

1

0

High-throughput

0.458

High

751810

SNU23
23 kDa U4/U6.U5 small nuclear ribonucleoprotein component

1

0

High-throughput

0.458

High

392492

HSP82
ATP-dependent molecular chaperone HSP82

1

2

High-throughput

0.445

High

481566

HSP73
Heat shock protein SSA3

1

4

High-throughput

0.445

High

517968

SSBP1
Single-stranded nucleic acid-binding protein

1

3

High-throughput

0.445

High

531677

RL4A
Large ribosomal subunit protein uL4A

1

1

High-throughput

0.445

High

675863

MAS5
Mitochondrial protein import protein MAS5

1

6

High-throughput

0.445

High

726900

HS104
Heat shock protein 104

1

1

High-throughput

0.445

High

741271

HSP7F
Heat shock protein homolog SSE1

1

7

High-throughput

0.445

High

742811

HSP79
Heat shock protein homolog SSE2

1

7

High-throughput

0.445

High

751735

SSZ1
Ribosome-associated complex subunit SSZ1

1

3

High-throughput

0.445

High

751740

CAJ1
Protein CAJ1

1

6

High-throughput

0.445

High

751743

HSP7E
Heat shock protein SSC3, mitochondrial

1

4

High-throughput

0.445

High

751769

APJ1
J domain-containing protein APJ1

1

6

High-throughput

0.445

High

751727

NU170
Nucleoporin NUP170

1

1

High-throughput

0.437

High

751734

IF5
Eukaryotic translation initiation factor 5

1

1

High-throughput

0.437

High

653845

HSP12
12 kDa heat shock protein

1

0

High-throughput

0.426

High

751753

PFD1
Prefoldin subunit 1

1

0

High-throughput

0.426

High

751755

GPN1
GPN-loop GTPase 1

1

0

High-throughput

0.426

High

751756

PFD3
Prefoldin subunit 3

1

0

High-throughput

0.426

High

751767

PFD4
Prefoldin subunit 4

1

0

High-throughput

0.426

High

751787

PFD5
Prefoldin subunit 5

1

0

High-throughput

0.426

High

751808

HSP42
Heat shock protein 42

1

0

High-throughput

0.426

High

577080

SEC63
Protein translocation protein SEC63

1

5

High-throughput

0.407

High

738195

NHP2
H/ACA ribonucleoprotein complex subunit NHP2

1

1

High-throughput

0.407

High

751738

TCPG
T-complex protein 1 subunit gamma

1

5

High-throughput

0.407

High

751739

TCPD
T-complex protein 1 subunit delta

1

6

High-throughput

0.407

High

408470

GAL80
Galactose/lactose metabolism regulatory protein GAL80

1

0

Small-scale

0.391

High

368635

PMG1
Phosphoglycerate mutase 1

2

0

Small-scale

0.300

High

751730

EXO84
Exocyst complex component EXO84

2

0

Small-scale

0.300

High

751761

YG1D
Probable transcriptional regulatory protein HAH1

2

0

Small-scale

0.300

High

149205

FOB1
DNA replication fork-blocking protein FOB1

1

0

Small-scale

0.251

Low

605955

GABAT
4-aminobutyrate aminotransferase

1

0

Small-scale

0.251

Low

751801

FMP45
SUR7 family protein FMP45

1

0

High-throughput

0.249

Low


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Last updated: 11Aug2025