Protein: MED25
Mediator of RNA polymerase II transcription subunit 25 (Activator interaction domain-containing protein 1) (Activator-recruited cofactor 92 kDa component) (ARC92) (Mediator complex subunit 25) (p78)

Gene: MED25
Organism: Homo sapiens
Interactions: 98
UniProt ID: Q71SY5

Gene Ontology (top 5):
GO:0000122 - negative regulation of transcription by RNA polymerase II
GO:0001223 - transcription coactivator binding
GO:0005634 - nucleus
GO:0005654 - nucleoplasm
GO:0005667 - transcription regulator complex

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

1259295

MED23
Mediator of RNA polymerase II transcription subunit 23

5

1

Small-scale

0.922

High

1259296

MED16
Mediator of RNA polymerase II transcription subunit 16

4

1

Small-scale

0.881

High

881553

CDK8
Cyclin-dependent kinase 8

10

3

Small-scale

0.751

High

48980

MED19
Mediator of RNA polymerase II transcription subunit 19

8

1

Small-scale

0.750

High

1151265

MED1
Mediator of RNA polymerase II transcription subunit 1

8

6

Small-scale

0.744

High

1259275

MED10
Mediator of RNA polymerase II transcription subunit 10

5

2

Small-scale

0.716

High

1259284

MED4
Mediator of RNA polymerase II transcription subunit 4

4

1

High-throughput

0.697

High

1259265

MED12
Mediator of RNA polymerase II transcription subunit 12

4

3

Small-scale

0.694

High

282396

MED24
Mediator of RNA polymerase II transcription subunit 24

4

1

Small-scale

0.691

High

396386

ESR1
Estrogen receptor

4

8

Small-scale

0.688

High

239592

MED14
Mediator of RNA polymerase II transcription subunit 14

4

5

Small-scale

0.683

High

1259287

MED29
Mediator of RNA polymerase II transcription subunit 29

3

1

High-throughput

0.675

High

287801

MED6
Mediator of RNA polymerase II transcription subunit 6

4

2

Small-scale

0.672

High

1259279

MED28
Mediator of RNA polymerase II transcription subunit 28

4

0

High-throughput

0.664

High

666858

CCNC
Cyclin-C

4

0

High-throughput

0.661

High

1108361

MED21
Mediator of RNA polymerase II transcription subunit 21

4

0

High-throughput

0.661

High

1259285

MED17
Mediator of RNA polymerase II transcription subunit 17

3

1

Small-scale

0.650

High

1259270

MED15
Mediator of RNA polymerase II transcription subunit 15

3

3

Small-scale

0.640

High

336007

MED26
Mediator of RNA polymerase II transcription subunit 26

3

0

Small-scale

0.638

High

1259286

MED9
Mediator of RNA polymerase II transcription subunit 9

3

0

Small-scale

0.634

High

1259278

CDK19
Cyclin-dependent kinase 19

2

3

High-throughput

0.628

High

219496

MED7
Mediator of RNA polymerase II transcription subunit 7

2

1

Small-scale

0.611

High

1259276

MED18
Mediator of RNA polymerase II transcription subunit 18

2

1

High-throughput

0.611

High

124614

EYA2
Protein phosphatase EYA2

2

1

High-throughput

0.606

High

132116

WWP2
NEDD4-like E3 ubiquitin-protein ligase WWP2

2

1

High-throughput

0.606

High

1259293

MED13
Mediator of RNA polymerase II transcription subunit 13

2

1

Small-scale

0.601

High

787877

SRBP1
Sterol regulatory element-binding protein 1

2

4

Small-scale

0.595

High

625831

RXRA
Retinoic acid receptor RXR-alpha

1

7

Small-scale

0.594

High

50292

ODAM
Odontogenic ameloblast-associated protein

2

0

High-throughput

0.580

High

907280

STA5B
Signal transducer and activator of transcription 5B

2

0

High-throughput

0.580

High

1061749

TLE5
TLE family member 5

2

0

High-throughput

0.580

High

1239362

ESRP1
Epithelial splicing regulatory protein 1

2

0

High-throughput

0.580

High

1259277

RUSC1
AP-4 complex accessory subunit RUSC1

2

0

High-throughput

0.580

High

1259288

WWOX
WW domain-containing oxidoreductase

2

0

High-throughput

0.580

High

1259289

SPN90
NCK-interacting protein with SH3 domain

2

0

High-throughput

0.580

High

1259294

SRTD3
SERTA domain-containing protein 3

2

0

High-throughput

0.580

High

520598

RARA
Retinoic acid receptor alpha

1

8

Small-scale

0.573

High

260108

BRD4
Bromodomain-containing protein 4

2

0

High-throughput

0.559

High

1259271

FEZ1
Fasciculation and elongation protein zeta-1

2

1

High-throughput

0.558

High

169610

HGS
Hepatocyte growth factor-regulated tyrosine kinase substrate

2

1

High-throughput

0.553

High

1259260

OXER1
Oxoeicosanoid receptor 1

2

1

High-throughput

0.553

High

1259262

TFG
Protein TFG

2

1

High-throughput

0.553

High

1259264

CBP
CREB-binding protein

1

1

Small-scale

0.552

High

1144042

TEBP
Prostaglandin E synthase 3

1

1

High-throughput

0.536

High

1241388

MED27
Mediator of RNA polymerase II transcription subunit 27

1

1

High-throughput

0.536

High

1259268

MED30
Mediator of RNA polymerase II transcription subunit 30

1

1

High-throughput

0.536

High

1259283

MED20
Mediator of RNA polymerase II transcription subunit 20

1

1

High-throughput

0.536

High

1259290

MED11
Mediator of RNA polymerase II transcription subunit 11

1

1

High-throughput

0.536

High

1259297

MED31
Mediator of RNA polymerase II transcription subunit 31

1

1

High-throughput

0.536

High

1259263

RORB
Nuclear receptor ROR-beta

1

5

High-throughput

0.515

High

455808

H10
Histone H1.0

1

0

High-throughput

0.513

High

542556

TOP2A
DNA topoisomerase 2-alpha

1

0

High-throughput

0.513

High

667352

RPB1
DNA-directed RNA polymerase II subunit RPB1

1

0

High-throughput

0.513

High

857485

NOTC1
Neurogenic locus notch homolog protein 1

1

0

High-throughput

0.513

High

958975

RPAB2
DNA-directed RNA polymerases I, II, and III subunit RPABC2

1

0

High-throughput

0.513

High

1057191

PRDX1
Peroxiredoxin-1

1

0

High-throughput

0.513

High

1149693

MED22
Mediator of RNA polymerase II transcription subunit 22

1

0

High-throughput

0.513

High

1163011

H2B2E
Histone H2B type 2-E

1

0

High-throughput

0.513

High

1259266

SYAP1
Synapse-associated protein 1

1

0

High-throughput

0.513

High

1259273

H2A1J
Histone H2A type 1-J

1

0

High-throughput

0.513

High

1259291

HOP2
Homologous-pairing protein 2 homolog

1

0

High-throughput

0.513

High

404155

GCR
Glucocorticoid receptor

1

8

Small-scale

0.508

High

362309

ABL1
Tyrosine-protein kinase ABL1

1

1

Small-scale

0.501

High

533295

THA
Thyroid hormone receptor alpha

1

7

Small-scale

0.481

High

1201918

Q58EY0
RAR related orphan receptor B isoform 1

1

6

High-throughput

0.481

High

1259299

CTDP1
RNA polymerase II subunit A C-terminal domain phosphatase

1

0

Small-scale

0.480

High

143074

NFIB
Nuclear factor 1 B-type

1

2

High-throughput

0.479

High

283496

TAD2A
Transcriptional adapter 2-alpha

1

2

High-throughput

0.479

High

474086

NFIC
Nuclear factor 1 C-type

1

4

High-throughput

0.479

High

1087825

NFIA
Nuclear factor 1 A-type

1

3

High-throughput

0.479

High

1153823

SMAD2
Mothers against decapentaplegic homolog 2

1

1

Small-scale

0.479

High

1153972

SMAD1
Mothers against decapentaplegic homolog 1

1

1

Small-scale

0.479

High

1211998

ZC3HD
Zinc finger CCCH domain-containing protein 13

1

1

High-throughput

0.479

High

1259259

MED25
Mediator of RNA polymerase II transcription subunit 25

1

1

Small-scale

0.479

High

1259261

ESR2
Estrogen receptor beta

1

7

High-throughput

0.479

High

1259281

SENP3
Sentrin-specific protease 3

1

0

High-throughput

0.460

High

1088886

TP53B
TP53-binding protein 1

1

0

High-throughput

0.458

High

1151345

TRIP4
Activating signal cointegrator 1

1

0

High-throughput

0.458

High

1259269

QKI
KH domain-containing RNA-binding protein QKI

1

0

High-throughput

0.458

High

1259272

EPAS1
Endothelial PAS domain-containing protein 1

1

0

Small-scale

0.458

High

1259282

STN1
CST complex subunit STN1

1

0

High-throughput

0.458

High

440131

LCK
Tyrosine-protein kinase Lck

1

1

Small-scale

0.457

High

533353

THB
Thyroid hormone receptor beta

1

7

Small-scale

0.457

High

1259280

Q9H2M2
Estrogen receptor 1

1

1

Small-scale

0.457

High

130521

SPT5H
Transcription elongation factor SPT5

1

2

High-throughput

0.445

High

351127

ECD
Protein ecdysoneless homolog

1

1

High-throughput

0.445

High

1259298

TRRAP
Transformation/transcription domain-associated protein

1

0

Small-scale

0.426

High

50338

F168B
Myelin-associated neurite-outgrowth inhibitor

2

0

High-throughput

0.316

High

70304

CRTP1
Cysteine-rich tail protein 1

2

0

High-throughput

0.316

High

1133110

LASP1
LIM and SH3 domain protein 1

2

0

High-throughput

0.316

High

1186568

KRA61
Keratin-associated protein 6-1

2

0

High-throughput

0.316

High

1198987

KLH12
Kelch-like protein 12

2

0

High-throughput

0.316

High

1258115

UBP54
Ubiquitin carboxyl-terminal hydrolase 54

2

0

High-throughput

0.316

High

1259267

PKHB2
Pleckstrin homology domain-containing family B member 2

2

0

High-throughput

0.316

High

1259292

PEF1
Peflin

2

0

High-throughput

0.316

High

1186756

KRA63
Keratin-associated protein 6-3

1

0

High-throughput

0.268

Low

1259274

GORS1
Golgi reassembly-stacking protein 1

1

0

High-throughput

0.232

Low


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Last updated: 11Aug2025