Protein: T2FA
General transcription factor IIF subunit 1 (General transcription factor IIF 74 kDa subunit) (Transcription initiation factor IIF subunit alpha) (TFIIF-alpha) (Transcription initiation factor RAP74)

Gene: T2FA, GTF2F1
Organism: Homo sapiens
Interactions: 162
UniProt ID: P35269

Gene Ontology (top 5):
GO:0001091 - RNA polymerase II general transcription initiation factor binding
GO:0001096 - TFIIF-class transcription factor complex binding
GO:0003677 - DNA binding
GO:0003723 - RNA binding
GO:0005634 - nucleus

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

569067

T2FB
General transcription factor IIF subunit 2

10

2

Small-scale

0.973

High

667194

RPB1
DNA-directed RNA polymerase II subunit RPB1

7

1

Small-scale

0.963

High

770440

CTDP1
RNA polymerase II subunit A C-terminal domain phosphatase

3

4

Small-scale

0.882

High

722897

RPB2
DNA-directed RNA polymerase II subunit RPB2

2

1

High-throughput

0.789

High

639676

TAF1
Transcription initiation factor TFIID subunit 1

5

1

Small-scale

0.721

High

770378

TF2B
Transcription initiation factor IIB

4

2

Small-scale

0.696

High

708877

T2EA
General transcription factor IIE subunit 1

4

1

Small-scale

0.694

High

628717

TBP
TATA-box-binding protein

4

0

Small-scale

0.671

High

371321

MYC
Myc proto-oncogene protein

3

1

Small-scale

0.667

High

610245

PRS6A
26S proteasome regulatory subunit 6A

3

3

High-throughput

0.667

High

770365

PSME3
Proteasome activator complex subunit 3

3

0

High-throughput

0.638

High

770420

DCC1
Sister chromatid cohesion protein DCC1

3

0

High-throughput

0.638

High

770364

RPAB2
DNA-directed RNA polymerases I, II, and III subunit RPABC2

2

1

High-throughput

0.628

High

770368

RPB7
DNA-directed RNA polymerase II subunit RPB7

2

1

High-throughput

0.628

High

487020

PARP1
Poly [ADP-ribose] polymerase 1

2

3

Small-scale

0.624

High

519793

ANDR
Androgen receptor

2

5

Small-scale

0.624

High

770413

STAC3
SH3 and cysteine-rich domain-containing protein 3

2

1

High-throughput

0.611

High

770363

PRPS1
Ribose-phosphate pyrophosphokinase 1

3

1

High-throughput

0.609

High

708928

T2EB
Transcription initiation factor IIE subunit beta

2

1

Small-scale

0.602

High

770351

PI42A
Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha

2

0

High-throughput

0.602

High

770393

PHF19
PHD finger protein 19

2

0

High-throughput

0.602

High

770394

JMJD6
Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6

2

0

High-throughput

0.602

High

770402

PCD20
Protocadherin-20

2

0

High-throughput

0.602

High

770432

SPNXA
Sperm protein associated with the nucleus on the X chromosome A

2

0

High-throughput

0.602

High

770407

TF2H4
General transcription factor IIH subunit 4

2

0

Small-scale

0.592

High

770382

MED21
Mediator of RNA polymerase II transcription subunit 21

2

0

Small-scale

0.585

High

770412

EAF1
ELL-associated factor 1

2

0

High-throughput

0.585

High

654622

TCEA1
Transcription elongation factor A protein 1

2

1

Small-scale

0.585

High

259534

BRD4
Bromodomain-containing protein 4

2

2

High-throughput

0.584

High

744635

TF2H1
General transcription factor IIH subunit 1

2

1

Small-scale

0.584

High

770352

CDK8
Cyclin-dependent kinase 8

2

2

Small-scale

0.584

High

770358

RPB11
DNA-directed RNA polymerase II subunit RPB11-a

2

1

Small-scale

0.584

High

770380

HNRPU
Heterogeneous nuclear ribonucleoprotein U

2

2

Small-scale

0.584

High

770355

CDK9
Cyclin-dependent kinase 9

2

0

Small-scale

0.577

High

395598

ESR1
Estrogen receptor

2

5

High-throughput

0.571

High

770388

KPCD1
Serine/threonine-protein kinase D1

2

1

High-throughput

0.571

High

549291

SRF
Serum response factor

2

0

Small-scale

0.569

High

340301

YETS4
YEATS domain-containing protein 4

1

1

Small-scale

0.561

High

563318

CFTR
Cystic fibrosis transmembrane conductance regulator

1

2

High-throughput

0.541

High

770360

DAPK1
Death-associated protein kinase 1

1

4

High-throughput

0.536

High

123612

MYO1C
Unconventional myosin-Ic

1

1

High-throughput

0.536

High

192791

RPB4
DNA-directed RNA polymerase II subunit RPB4

1

1

High-throughput

0.536

High

484957

ROA1
Heterogeneous nuclear ribonucleoprotein A1

1

1

High-throughput

0.536

High

621567

RPB3
DNA-directed RNA polymerase II subunit RPB3

1

2

Small-scale

0.536

High

621825

RPAB1
DNA-directed RNA polymerases I, II, and III subunit RPABC1

1

1

High-throughput

0.536

High

770361

RPAB4
DNA-directed RNA polymerases I, II, and III subunit RPABC4

1

1

High-throughput

0.536

High

770379

CDK16
Cyclin-dependent kinase 16

1

2

High-throughput

0.536

High

770384

NOG2
Nucleolar GTP-binding protein 2

1

2

High-throughput

0.536

High

770386

TF2H3
General transcription factor IIH subunit 3

1

1

High-throughput

0.536

High

770389

TAF11
Transcription initiation factor TFIID subunit 11

1

0

Small-scale

0.527

High

571886

TRI27
Zinc finger protein RFP

1

0

High-throughput

0.518

High

636707

PTN
Pleiotrophin

1

0

High-throughput

0.518

High

407591

S100B
Protein S100-B

1

0

High-throughput

0.513

High

611785

RL35A
Large ribosomal subunit protein eL33

1

0

High-throughput

0.513

High

770377

SERF2
Small EDRK-rich factor 2

1

0

High-throughput

0.513

High

770381

MD2L1
Mitotic spindle assembly checkpoint protein MAD2A

1

0

High-throughput

0.513

High

770383

THOC5
THO complex subunit 5

1

0

High-throughput

0.513

High

770392

LBH
Protein LBH

1

0

High-throughput

0.513

High

770406

SNR27
U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein

1

0

High-throughput

0.513

High

770409

KCD12
BTB/POZ domain-containing protein KCTD12

1

0

High-throughput

0.513

High

770415

KIF2C
Kinesin-like protein KIF2C

1

0

High-throughput

0.513

High

770417

TCAL7
Transcription elongation factor A protein-like 7

1

0

High-throughput

0.513

High

770418

MMTA2
Multiple myeloma tumor-associated protein 2

1

0

High-throughput

0.513

High

770426

SPRTN
DNA-dependent metalloprotease SPRTN

1

0

High-throughput

0.513

High

770429

MED20
Mediator of RNA polymerase II transcription subunit 20

1

0

High-throughput

0.513

High

770430

MED4
Mediator of RNA polymerase II transcription subunit 4

1

0

High-throughput

0.513

High

770431

PRD11
PR domain-containing protein 11

1

0

High-throughput

0.513

High

770443

RM42
Large ribosomal subunit protein mL42

1

0

High-throughput

0.513

High

316359

RMP
Unconventional prefoldin RPB5 interactor 1

1

0

Small-scale

0.512

High

251523

CCNT1
Cyclin-T1

1

1

Small-scale

0.501

High

370587

FOS
Protein c-Fos

1

1

Small-scale

0.501

High

693252

ARNT
Aryl hydrocarbon receptor nuclear translocator

1

3

Small-scale

0.501

High

770345

AHR
Aryl hydrocarbon receptor

1

4

Small-scale

0.501

High

595970

H12
Histone H1.2

1

0

High-throughput

0.493

High

549543

PRPS2
Ribose-phosphate pyrophosphokinase 2

1

1

High-throughput

0.489

High

770441

WIF1
Wnt inhibitory factor 1

1

1

High-throughput

0.489

High

254463

MAGC1
Melanoma-associated antigen C1

1

0

Small-scale

0.486

High

362186

ABL1
Tyrosine-protein kinase ABL1

1

0

Small-scale

0.480

High

429404

JUN
Transcription factor Jun

1

0

Small-scale

0.480

High

770344

MSX2
Homeobox protein MSX-2

1

0

Small-scale

0.480

High

770359

TF2AA
Transcription initiation factor IIA subunit 1

1

0

Small-scale

0.480

High

770375

CSK21
Casein kinase II subunit alpha

1

0

Small-scale

0.480

High

190834

RPA34
DNA-directed RNA polymerase I subunit RPA34

1

1

High-throughput

0.479

High

254334

CC14B
Dual specificity protein phosphatase CDC14B

1

3

High-throughput

0.479

High

302405

RNF8
E3 ubiquitin-protein ligase RNF8

1

2

Small-scale

0.479

High

348989

LATS1
Serine/threonine-protein kinase LATS1

1

2

High-throughput

0.479

High

411471

NTRK1
High affinity nerve growth factor receptor

1

4

High-throughput

0.479

High

413304

P53
Cellular tumor antigen p53

1

1

High-throughput

0.479

High

588045

RFA2
Replication protein A 32 kDa subunit

1

2

High-throughput

0.479

High

605575

KAPCA
cAMP-dependent protein kinase catalytic subunit alpha

1

2

High-throughput

0.479

High

666803

CCNC
Cyclin-C

1

1

Small-scale

0.479

High

694759

RFA1
Replication protein A 70 kDa DNA-binding subunit

1

1

High-throughput

0.479

High

766241

PHB1
Prohibitin 1

1

2

High-throughput

0.479

High

767540

RFA3
Replication protein A 14 kDa subunit

1

1

High-throughput

0.479

High

770350

ATRX
Transcriptional regulator ATRX

1

3

Small-scale

0.479

High

770353

CENPA
Histone H3-like centromeric protein A

1

2

High-throughput

0.479

High

770366

WDR5
WD repeat-containing protein 5

1

5

High-throughput

0.479

High

770367

UBE2H
Ubiquitin-conjugating enzyme E2 H

1

1

High-throughput

0.479

High

770370

H2B1C
Histone H2B type 1-C/E/F/G/I

1

2

High-throughput

0.479

High

770373

RL10A
Large ribosomal subunit protein uL1

1

1

High-throughput

0.479

High

770376

H31
Histone H3.1

1

2

High-throughput

0.479

High

770398

PARPT
Protein mono-ADP-ribosyltransferase TIPARP

1

1

High-throughput

0.479

High

770401

SLX4
Structure-specific endonuclease subunit SLX4

1

1

High-throughput

0.479

High

770408

KAT6A
Histone acetyltransferase KAT6A

1

3

High-throughput

0.479

High

770410

SNR40
U5 small nuclear ribonucleoprotein 40 kDa protein

1

5

High-throughput

0.479

High

770411

DDRGK
DDRGK domain-containing protein 1

1

1

High-throughput

0.479

High

770423

MK67I
MKI67 FHA domain-interacting nucleolar phosphoprotein

1

2

High-throughput

0.479

High

770425

RPA49
DNA-directed RNA polymerase I subunit RPA49

1

1

High-throughput

0.479

High

770436

ZRAN1
Ubiquitin thioesterase ZRANB1

1

1

High-throughput

0.479

High

770422

B2L13
Bcl-2-like protein 13

1

0

Small-scale

0.460

High

140776

NOP56
Nucleolar protein 56

1

0

High-throughput

0.458

High

172512

XPO1
Exportin-1

1

0

High-throughput

0.458

High

533889

IRF1
Interferon regulatory factor 1

1

0

Small-scale

0.458

High

557898

XRCC6
X-ray repair cross-complementing protein 6

1

0

High-throughput

0.458

High

611649

ERCC2
General transcription and DNA repair factor IIH helicase subunit XPD

1

0

Small-scale

0.458

High

622770

ERCC3
General transcription and DNA repair factor IIH helicase/translocase subunit XPB

1

0

High-throughput

0.458

High

697020

APEX1
DNA repair nuclease/redox regulator APEX1

1

0

High-throughput

0.458

High

769816

RFC2
Replication factor C subunit 2

1

0

High-throughput

0.458

High

770346

PRS7
26S proteasome regulatory subunit 7

1

0

Small-scale

0.458

High

770348

COIL
Coilin

1

0

High-throughput

0.458

High

770356

RL14
Large ribosomal subunit protein eL14

1

0

High-throughput

0.458

High

770357

IMA1
Importin subunit alpha-1

1

0

High-throughput

0.458

High

770362

ANM2
Protein arginine N-methyltransferase 2

1

0

High-throughput

0.458

High

770369

RL23A
Large ribosomal subunit protein uL23

1

0

High-throughput

0.458

High

770371

RS24
Small ribosomal subunit protein eS24

1

0

High-throughput

0.458

High

770372

RL31
Large ribosomal subunit protein eL31

1

0

High-throughput

0.458

High

770385

TF2H2
General transcription factor IIH subunit 2

1

0

High-throughput

0.458

High

770387

U5S1
116 kDa U5 small nuclear ribonucleoprotein component

1

0

High-throughput

0.458

High

770396

TF2H5
General transcription factor IIH subunit 5

1

0

High-throughput

0.458

High

770397

MEPCE
7SK snRNA methylphosphate capping enzyme

1

0

High-throughput

0.458

High

770399

SLN11
Schlafen family member 11

1

0

High-throughput

0.458

High

770403

SERB1
SERPINE1 mRNA-binding protein 1

1

0

High-throughput

0.458

High

770405

PLPR1
Phospholipid phosphatase-related protein type 1

1

0

High-throughput

0.458

High

770414

TBX5
T-box transcription factor TBX5

1

0

High-throughput

0.458

High

770419

DDX23
Probable ATP-dependent RNA helicase DDX23

1

0

High-throughput

0.458

High

770424

UBE2O
(E3-independent) E2 ubiquitin-conjugating enzyme

1

0

High-throughput

0.458

High

770433

TE2IP
Telomeric repeat-binding factor 2-interacting protein 1

1

0

High-throughput

0.458

High

770437

ASF1A
Histone chaperone ASF1A

1

0

High-throughput

0.458

High

770438

ZN330
Zinc finger protein 330

1

0

High-throughput

0.458

High

770347

BRCA1
Breast cancer type 1 susceptibility protein

1

2

Small-scale

0.445

High

770349

CDN2A
Cyclin-dependent kinase inhibitor 2A

1

2

High-throughput

0.445

High

770395

TRI67
Tripartite motif-containing protein 67

1

2

High-throughput

0.437

High

770428

PKHA4
Pleckstrin homology domain-containing family A member 4

1

1

High-throughput

0.437

High

770354

CEBPA
CCAAT/enhancer-binding protein alpha

1

1

High-throughput

0.408

High

770421

C1QT2
Complement C1q tumor necrosis factor-related protein 2

3

0

High-throughput

0.347

High

239013

ZNRD2
Protein ZNRD2

2

0

High-throughput

0.328

High

770416

FEZ1
Fasciculation and elongation protein zeta-1

1

0

High-throughput

0.282

High

346880

BAG2
BAG family molecular chaperone regulator 2

1

0

High-throughput

0.279

Low

387799

FIBB
Fibrinogen beta chain [Cleaved into: Fibrinopeptide B; Fibrinogen beta chain]

1

0

High-throughput

0.279

Low

725863

FGF9
Fibroblast growth factor 9

1

0

High-throughput

0.279

Low

770391

P5CR3
Pyrroline-5-carboxylate reductase 3

1

0

High-throughput

0.279

Low

770400

CCD71
Coiled-coil domain-containing protein 71

1

0

High-throughput

0.279

Low

770427

CAD23
Cadherin-23

1

0

High-throughput

0.279

Low

770434

PEN2
Gamma-secretase subunit PEN-2

1

0

High-throughput

0.279

Low

770435

GRHPR
Glyoxylate reductase/hydroxypyruvate reductase

1

0

High-throughput

0.279

Low

770439

ATG4B
Cysteine protease ATG4B

1

0

High-throughput

0.279

Low

770442

SPCS1
Signal peptidase complex subunit 1

1

0

High-throughput

0.279

Low

178613

DEGS1
Sphingolipid delta(4)-desaturase DES1

1

0

High-throughput

0.249

Low

472283

CFAH
Complement factor H

1

0

Small-scale

0.249

Low

770374

ACTC
Actin, alpha cardiac muscle 1

1

0

High-throughput

0.249

Low

770390

CDC37
Hsp90 co-chaperone Cdc37

1

0

High-throughput

0.249

Low

770404

ARK74
Aflatoxin B1 aldehyde reductase member 4

1

0

High-throughput

0.232

Low


Service provided by Combinatics, Japan

Last updated: 11Aug2025