Protein: BNI1
Protein BNI1 (Pointed projection formation protein 3) (Sensitive to high expression protein 5) (Synthetic lethal 39)

Gene: BNI1
Organism: Saccharomyces cerevisiae S288C
Interactions: 240
UniProt ID: P41832

Gene Ontology (top 5):
GO:0000131 - incipient cellular bud site
GO:0000132 - establishment of mitotic spindle orientation
GO:0000133 - polarisome
GO:0005522 - profilin binding
GO:0005628 - prospore membrane

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

836281

BNI1
Protein BNI1

6

14

Small-scale

0.939

High

618352

CDC42
Cell division control protein 42

5

20

Small-scale

0.919

High

836313

ACT
Actin

5

18

Small-scale

0.916

High

448546

RHO1
GTP-binding protein RHO1

3

20

Small-scale

0.843

High

736113

ARP2
Actin-related protein 2

2

19

High-throughput

0.799

High

824648

BNR1
BNI1-related protein 1

2

14

Small-scale

0.757

High

835533

BUD6
Bud site selection protein 6

8

2

Small-scale

0.745

High

454623

PROF
Profilin

4

20

Small-scale

0.700

High

836285

YFI6
Uncharacterized protein YFR016C

3

5

Small-scale

0.655

High

836290

ARP3
Actin-related protein 3

1

20

High-throughput

0.647

High

364593

CDK1
Cyclin-dependent kinase 1

3

0

Small-scale

0.641

High

524263

HSP71
Heat shock protein SSA1

3

7

High-throughput

0.641

High

836311

ZDS2
Protein ZDS2

3

1

High-throughput

0.639

High

526947

HSP72
Heat shock protein SSA2

3

7

High-throughput

0.633

High

679795

CDC48
Cell division control protein 48

2

6

High-throughput

0.628

High

836316

RHO3
GTP-binding protein RHO3

1

20

Small-scale

0.621

High

548282

FUS1
Nuclear fusion protein FUS1

2

1

Small-scale

0.603

High

836317

NUM1
Nuclear migration protein NUM1

2

8

Small-scale

0.601

High

579581

HSC82
ATP-dependent molecular chaperone HSC82

2

6

High-throughput

0.595

High

836347

KAR
NADPH-dependent alpha-keto amide reductase

2

1

High-throughput

0.595

High

743345

FIMB
Fimbrin

2

2

Small-scale

0.594

High

757704

EDE1
EH domain-containing and endocytosis protein 1

2

4

Small-scale

0.594

High

836296

GYP3
GTPase-activating protein GYP3

2

3

Small-scale

0.594

High

779769

DYHC
Dynein heavy chain, cytoplasmic

2

2

Small-scale

0.584

High

815471

RSP5
E3 ubiquitin-protein ligase RSP5

1

4

Small-scale

0.578

High

778996

MYO3
Myosin-3

1

8

High-throughput

0.573

High

836318

CDC14
Tyrosine-protein phosphatase CDC14

1

6

Small-scale

0.561

High

654727

SPA2
Protein SPA2

1

3

Small-scale

0.552

High

394656

EF1A
Elongation factor 1-alpha

1

0

Small-scale

0.537

High

836328

TCB3
Tricalbin-3

1

2

High-throughput

0.536

High

792414

BOI1
BEM1-interacting protein 1

1

2

Small-scale

0.527

High

792840

ANM1
Protein arginine N-methyltransferase 1

2

2

High-throughput

0.526

High

836332

MYO5
Myosin-5

1

6

High-throughput

0.508

High

836335

CYK2
Cytokinesis protein 2

1

11

Small-scale

0.508

High

367056

ATPB
ATP synthase subunit beta, mitochondrial

1

2

High-throughput

0.501

High

449137

CALM
Calmodulin

1

4

High-throughput

0.501

High

560718

KIN2
Serine/threonine-protein kinase KIN2

1

2

High-throughput

0.501

High

562963

CAPZB
F-actin-capping protein subunit beta

1

1

High-throughput

0.501

High

607768

HXKG
Glucokinase-1

1

1

High-throughput

0.501

High

670285

NAP1
Nucleosome assembly protein

1

1

High-throughput

0.501

High

670835

SIS1
Protein SIS1

1

4

High-throughput

0.501

High

672249

CDC10
Cell division control protein 10

1

3

High-throughput

0.501

High

673379

NFS1
Cysteine desulfurase, mitochondrial

1

1

High-throughput

0.501

High

678111

SYP1
Suppressor of yeast profilin deletion

1

1

High-throughput

0.501

High

703774

CAPZA
F-actin-capping protein subunit alpha

1

1

High-throughput

0.501

High

705774

IDH1
Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial

1

2

High-throughput

0.501

High

736991

CDC3
Cell division control protein 3

1

4

High-throughput

0.501

High

744876

SLA1
Actin cytoskeleton-regulatory complex protein SLA1

1

2

High-throughput

0.501

High

777212

KES1
Oxysterol-binding protein homolog 4

1

2

High-throughput

0.501

High

779490

YKT6
Synaptobrevin homolog YKT6

1

1

High-throughput

0.501

High

798149

ARPC1
Actin-related protein 2/3 complex subunit 1

1

4

High-throughput

0.501

High

798416

RGD1
RHO GTPase-activating protein RGD1

1

5

High-throughput

0.501

High

805404

BZZ1
Protein BZZ1

1

11

High-throughput

0.501

High

811330

CAJ1
Protein CAJ1

1

5

High-throughput

0.501

High

814215

RV167
Reduced viability upon starvation protein 167

1

7

High-throughput

0.501

High

825403

PRK1
Actin-regulating kinase PRK1

1

3

Small-scale

0.501

High

836286

LSB3
LAS seventeen-binding protein 3

1

7

High-throughput

0.501

High

836288

AIP1
Actin-interacting protein 1

1

2

High-throughput

0.501

High

836304

TWF1
Twinfilin-1

1

1

High-throughput

0.501

High

836327

STE20
Serine/threonine-protein kinase STE20

1

1

Small-scale

0.501

High

836330

SNX41
Sorting nexin-41

1

4

High-throughput

0.501

High

836340

CORO
Coronin-like protein

1

4

High-throughput

0.501

High

836349

VPS13
Intermembrane lipid transfer protein VPS13

1

1

High-throughput

0.501

High

836357

SGT2
Small glutamine-rich tetratricopeptide repeat-containing protein 2

1

3

High-throughput

0.501

High

836367

MSB4
GTPase-activating protein MSB4

1

3

Small-scale

0.501

High

637835

MSB1
Morphogenesis-related protein MSB1

1

0

High-throughput

0.486

High

722022

XPO1
Exportin-1

1

6

Small-scale

0.481

High

816032

BOI2
BEM1-interacting protein 2

1

4

Small-scale

0.481

High

817729

TSC11
Target of rapamycin complex 2 subunit TSC11

1

2

High-throughput

0.481

High

836292

SGM1
Protein SGM1

1

7

Small-scale

0.481

High

836302

MLC1
Myosin light chain 1

1

4

Small-scale

0.481

High

836321

OSH6
Oxysterol-binding protein homolog 6

1

2

Small-scale

0.481

High

361444

SODC
Superoxide dismutase [Cu-Zn]

1

0

High-throughput

0.480

High

452516

PUR2
Bifunctional purine biosynthetic protein ADE5,7 [Includes: Phosphoribosylamine--glycine ligase

1

0

High-throughput

0.480

High

510972

IPB2
Protease B inhibitor 2

1

0

High-throughput

0.480

High

599370

FUS3
Mitogen-activated protein kinase FUS3

1

0

Small-scale

0.480

High

605109

TPM1
Tropomyosin-1

1

0

High-throughput

0.480

High

653871

HSP12
12 kDa heat shock protein

1

0

High-throughput

0.480

High

721106

LCF1
Long-chain-fatty-acid--CoA ligase 1

1

0

High-throughput

0.480

High

746288

UGPA1
UTP--glucose-1-phosphate uridylyltransferase

1

0

High-throughput

0.480

High

750337

ARPC4
Actin-related protein 2/3 complex subunit 4

1

0

High-throughput

0.480

High

751652

NTF2
Nuclear transport factor 2

1

0

High-throughput

0.480

High

763688

GCS1
ADP-ribosylation factor GTPase-activating protein GCS1

1

0

High-throughput

0.480

High

788421

PGM2
Phosphoglucomutase 2

1

0

High-throughput

0.480

High

791969

PUR92
Bifunctional purine biosynthesis protein ADE17 [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase

1

0

High-throughput

0.480

High

792357

AHP1
Peroxiredoxin AHP1

1

0

High-throughput

0.480

High

797922

SDS24
Protein SDS24

1

0

High-throughput

0.480

High

813285

FHP
Flavohemoprotein

1

0

High-throughput

0.480

High

823244

SEC9
Protein transport protein SEC9

1

0

High-throughput

0.480

High

825841

ARPC5
Actin-related protein 2/3 complex subunit 5

1

0

High-throughput

0.480

High

825963

GVP36
Protein GVP36

1

0

High-throughput

0.480

High

836282

GSHR
Glutathione reductase

1

0

High-throughput

0.480

High

836324

COFI
Cofilin

1

0

High-throughput

0.480

High

836337

ARPC3
Actin-related protein 2/3 complex subunit 3

1

0

High-throughput

0.480

High

836345

PEX19
Peroxisomal membrane protein import receptor PEX19

1

0

High-throughput

0.480

High

836350

MTAP
S-methyl-5'-thioadenosine phosphorylase

1

0

High-throughput

0.480

High

836351

ZEO1
Protein ZEO1

1

0

High-throughput

0.480

High

836353

MCA1
Metacaspase-1

1

0

High-throughput

0.480

High

836365

MDY2
Ubiquitin-like protein MDY2

1

0

High-throughput

0.480

High

836369

PST2
Protoplast secreted protein 2

1

0

High-throughput

0.480

High

149819

RS9A
Small ribosomal subunit protein uS4A

1

1

High-throughput

0.479

High

656502

KC11
Casein kinase I homolog 1

1

2

High-throughput

0.479

High

796419

TRM7
tRNA

1

1

High-throughput

0.479

High

805462

DMA1
E3 ubiquitin-protein ligase DMA1

1

3

Small-scale

0.479

High

836310

DMA2
E3 ubiquitin-protein ligase DMA2

1

3

Small-scale

0.479

High

836320

MTD1
Methylenetetrahydrofolate dehydrogenase [NAD(+)]

1

1

High-throughput

0.479

High

154359

YO304
Uncharacterized protein YOR304C-A

1

0

Small-scale

0.460

High

624853

EXO70
Exocyst complex component EXO70

1

0

Small-scale

0.460

High

644800

CLH
Clathrin heavy chain

1

0

Small-scale

0.460

High

746167

SEC8
Exocyst complex component SEC8

1

0

Small-scale

0.460

High

780551

SHE2
SWI5-dependent HO expression protein 2

1

0

Small-scale

0.460

High

836297

BRO1
Vacuolar-sorting protein BRO1

1

0

High-throughput

0.460

High

836315

SEC5
Exocyst complex component SEC5

1

0

Small-scale

0.460

High

836342

HDA2
HDA1 complex subunit 2

1

0

High-throughput

0.460

High

435162

RS3
Small ribosomal subunit protein uS3

1

0

High-throughput

0.458

High

674682

RS2
Small ribosomal subunit protein uS5

1

0

High-throughput

0.458

High

747905

SMY2
Protein SMY2

1

0

High-throughput

0.458

High

812399

LAA1
AP-1 accessory protein LAA1

1

0

High-throughput

0.458

High

817207

FIR1
Factor interacting with REF2

1

0

High-throughput

0.458

High

822954

RFC4
Replication factor C subunit 4

1

0

High-throughput

0.458

High

836301

YIP5
Protein YIP5

1

0

High-throughput

0.458

High

836307

BUD17
Putative pyridoxal kinase BUD17

1

0

High-throughput

0.458

High

836325

YD090
Uncharacterized membrane protein YDR090C

1

0

High-throughput

0.458

High

836329

SCC2
Sister chromatid cohesion protein 2

1

0

High-throughput

0.458

High

836334

DYN3
Cytoplasmic dynein intermediate light chain DYN3

1

0

High-throughput

0.458

High

836343

GIC2
GTPase-interacting component 2

1

0

Small-scale

0.458

High

836348

FMP45
SUR7 family protein FMP45

1

0

High-throughput

0.458

High

836363

RIO1
Serine/threonine-protein kinase RIO1

1

0

High-throughput

0.458

High

836370

YPR1
Aldehyde reductase YPR1

1

0

High-throughput

0.458

High

486475

TBA3
Tubulin alpha-3 chain

1

4

High-throughput

0.457

High

488621

RIR2
Ribonucleoside-diphosphate reductase small chain 1

1

1

High-throughput

0.457

High

590655

HSP26
Heat shock protein 26

1

1

High-throughput

0.457

High

592318

VATB
V-type proton ATPase subunit B

1

2

High-throughput

0.457

High

633555

SAR1
Small COPII coat GTPase SAR1

1

7

High-throughput

0.457

High

638727

RIR1
Ribonucleoside-diphosphate reductase large chain 1

1

1

High-throughput

0.457

High

639560

RIR3
Ribonucleoside-diphosphate reductase large chain 2

1

1

High-throughput

0.457

High

693498

CBPS
Carboxypeptidase S

1

1

High-throughput

0.457

High

701540

IDH2
Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial

1

2

High-throughput

0.457

High

780153

SDS22
Protein phosphatase 1 regulatory subunit SDS22

1

3

High-throughput

0.457

High

810706

TCPB
T-complex protein 1 subunit beta

1

1

High-throughput

0.457

High

836287

DUG1
Cys-Gly metallodipeptidase DUG1

1

1

High-throughput

0.457

High

836289

BBC1
Myosin tail region-interacting protein MTI1

1

3

High-throughput

0.457

High

836298

RIR4
Ribonucleoside-diphosphate reductase small chain 2

1

1

High-throughput

0.457

High

836336

CD123
Translation initiation factor eIF2 assembly protein

1

2

High-throughput

0.457

High

836344

UBX5
UBX domain-containing protein 5

1

1

High-throughput

0.457

High

836360

AIM7
Protein AIM7

1

1

High-throughput

0.457

High

836366

YD061
Uncharacterized ABC transporter ATP-binding protein YDR061W

1

3

High-throughput

0.457

High

836368

HSP42
Heat shock protein 42

1

1

High-throughput

0.457

High

392894

HSP82
ATP-dependent molecular chaperone HSP82

1

6

High-throughput

0.445

High

481656

HSP73
Heat shock protein SSA3

1

7

High-throughput

0.445

High

545243

SSB1
Ribosome-associated molecular chaperone SSB1

1

7

High-throughput

0.445

High

676085

MAS5
Mitochondrial protein import protein MAS5

1

3

High-throughput

0.445

High

727110

HS104
Heat shock protein 104

1

2

High-throughput

0.445

High

741779

HSP7F
Heat shock protein homolog SSE1

1

9

High-throughput

0.445

High

819020

SSB2
Ribosome-associated molecular chaperone SSB2

1

7

High-throughput

0.445

High

671331

CANB
Calcineurin subunit B

1

3

Small-scale

0.439

High

700113

PRP45
Pre-mRNA-processing protein 45

1

3

Small-scale

0.439

High

750699

PRS6A
26S proteasome regulatory subunit 6A

1

4

High-throughput

0.439

High

836305

YG3A
Uncharacterized protein YGR130C

1

9

High-throughput

0.439

High

836323

CENPP
Inner kinetochore subunit CTF19

1

1

High-throughput

0.439

High

636932

FAR1
Cyclin-dependent kinase inhibitor FAR1

1

0

Small-scale

0.438

High

707379

TF2B
Transcription initiation factor IIB

1

1

High-throughput

0.437

High

712998

MCM2
DNA replication licensing factor MCM2

1

1

High-throughput

0.437

High

778906

NNK1
Nitrogen network kinase 1

1

2

High-throughput

0.437

High

800658

KPR2
Ribose-phosphate pyrophosphokinase 2

1

1

High-throughput

0.437

High

801781

KPR3
Ribose-phosphate pyrophosphokinase 3

1

1

High-throughput

0.437

High

836293

SSN8
RNA polymerase II holoenzyme cyclin-like subunit

1

1

High-throughput

0.437

High

836314

H3
Histone H3

1

6

High-throughput

0.437

High

836322

MLP1
Protein MLP1

1

4

High-throughput

0.437

High

836371

CMR1
DNA damage-binding protein CMR1

1

1

High-throughput

0.437

High

790450

PAM1
Protein PAM1

1

0

High-throughput

0.426

High

815933

PP2C2
Protein phosphatase 2C homolog 2

1

0

High-throughput

0.426

High

836364

NCE2
Non-classical export protein 2

1

0

High-throughput

0.426

High

613860

ERD2
ER lumen protein-retaining receptor

1

2

High-throughput

0.407

High

634248

NUP1
Nucleoporin NUP1

1

1

High-throughput

0.407

High

703827

LAC1
Ceramide synthase LAC1

1

1

High-throughput

0.407

High

752382

HSP78
Heat shock protein 78, mitochondrial

1

2

High-throughput

0.407

High

836291

DPH4
Diphthamide biosynthesis protein 4

1

4

High-throughput

0.407

High

836356

FAL1
ATP-dependent RNA helicase FAL1

2

0

High-throughput

0.307

High

836283

MPG1
Mannose-1-phosphate guanyltransferase

1

0

High-throughput

0.279

Low

836306

RT35
Small ribosomal subunit protein mS45

1

0

High-throughput

0.279

Low

360994

CCPR
Cytochrome c peroxidase, mitochondrial

1

0

High-throughput

0.261

Low

361486

SODM
Superoxide dismutase [Mn], mitochondrial

1

0

High-throughput

0.261

Low

366717

HIS2
Histidine biosynthesis trifunctional protein [Includes: Phosphoribosyl-AMP cyclohydrolase

1

0

High-throughput

0.261

Low

366817

IPYR
Inorganic pyrophosphatase

1

0

High-throughput

0.261

Low

571355

CPYI
Carboxypeptidase Y inhibitor

1

0

High-throughput

0.261

Low

598329

HEMH
Ferrochelatase, mitochondrial

1

0

High-throughput

0.261

Low

610165

PDI
Protein disulfide-isomerase

1

0

High-throughput

0.261

Low

725888

CYS3
Cystathionine gamma-lyase

1

0

High-throughput

0.261

Low

745240

COX13
Cytochrome c oxidase subunit 13, mitochondrial

1

0

High-throughput

0.261

Low

757743

PIN4
RNA-binding protein PIN4

1

0

High-throughput

0.261

Low

776322

MRP8
Uncharacterized protein MRP8

1

0

High-throughput

0.261

Low

799706

CUE1
Coupling of ubiquitin conjugation to ER degradation protein 1

1

0

High-throughput

0.261

Low

805035

SDO1L
Restriction of telomere capping protein 3

1

0

High-throughput

0.261

Low

820348

YNU8
Uncharacterized protein YNL208W

1

0

High-throughput

0.261

Low

821970

PSA6
Proteasome subunit alpha type-6

1

0

High-throughput

0.261

Low

825735

MAM33
Mitochondrial acidic protein MAM33

1

0

High-throughput

0.261

Low

836284

WWM1
WW domain-containing protein WWM1

1

0

High-throughput

0.261

Low

836309

YNN4
Uncharacterized protein YNL134C

1

0

High-throughput

0.261

Low

836319

TPS1
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit

1

0

High-throughput

0.261

Low

836331

YM54
Uncharacterized protein YMR196W

1

0

High-throughput

0.261

Low

836333

IVY1
Protein IVY1

1

0

High-throughput

0.261

Low

836352

CUE5
Ubiquitin-binding protein CUE5

1

0

High-throughput

0.261

Low

836358

DCS2
Inactive diphosphatase DCS2

1

0

High-throughput

0.261

Low

836361

ATPD
ATP synthase subunit delta, mitochondrial

1

0

High-throughput

0.261

Low

836372

HUG1
MEC1-mediated checkpoint protein HUG1

1

0

High-throughput

0.261

Low

816067

ACA1
ATF/CREB activator 1

1

0

High-throughput

0.251

Low

836303

KNL1
Outer kinetochore KNL1 complex subunit SPC105

1

0

High-throughput

0.251

Low

149214

FOB1
DNA replication fork-blocking protein FOB1

1

0

High-throughput

0.249

Low

561488

TBP
TATA-box-binding protein

1

0

High-throughput

0.249

Low

585559

BGL2
Glucan 1,3-beta-glucosidase

1

0

High-throughput

0.249

Low

678812

SRB8
Mediator of RNA polymerase II transcription subunit 12

1

0

High-throughput

0.249

Low

713582

RIF1
Telomere length regulator protein RIF1

1

0

High-throughput

0.249

Low

763766

THI80
Thiamine pyrophosphokinase

1

0

High-throughput

0.249

Low

781562

NTR2
Pre-mRNA-splicing factor NTR2

1

0

High-throughput

0.249

Low

794944

YBI1
Uncharacterized protein YBL081W

1

0

High-throughput

0.249

Low

797750

AIM4
Altered inheritance of mitochondria protein 4

1

0

High-throughput

0.249

Low

797887

CENPU
Inner kinetochore subunit AME1

1

0

High-throughput

0.249

Low

807817

SSN2
Mediator of RNA polymerase II transcription subunit 13

1

0

Small-scale

0.249

Low

817304

PHM8
Phosphate metabolism protein 8

1

0

High-throughput

0.249

Low

836295

BUL1
Ubiquitin ligase-binding protein BUL1

1

0

High-throughput

0.249

Low

836299

RL4P
Large ribosomal subunit protein uL4m

1

0

High-throughput

0.249

Low

836300

PAN2
PAN2-PAN3 deadenylation complex catalytic subunit PAN2

1

0

High-throughput

0.249

Low

836312

DOS2
Protein DOS2

1

0

High-throughput

0.249

Low

836326

DIG2
Down-regulator of invasive growth 2

1

0

High-throughput

0.249

Low

836338

YL257
Uncharacterized protein YLR257W

1

0

High-throughput

0.249

Low

836339

YPQ2
Vacuolar arginine/histidine antiporter YPQ2

1

0

High-throughput

0.249

Low

836341

URN1
Pre-mRNA-splicing factor URN1

1

0

High-throughput

0.249

Low

836346

YD073
UPF0592 protein YDL073W

1

0

High-throughput

0.249

Low

836354

PMG2
Phosphoglycerate mutase 2

1

0

High-throughput

0.249

Low

836355

TSR3
18S rRNA aminocarboxypropyltransferase

1

0

High-throughput

0.249

Low

836359

CMR3
Zinc finger protein CRM3

1

0

High-throughput

0.249

Low

836362

RFM1
Repression factor of MSEs protein 1

1

0

High-throughput

0.249

Low

150378

YP089
Dilute domain-containing protein YPR089W

1

0

High-throughput

0.232

Low

676646

SGF29
SAGA complex subunit SGF29

1

0

High-throughput

0.232

Low

806470

NACA
Nascent polypeptide-associated complex subunit alpha

1

0

High-throughput

0.232

Low

836294

PFD3
Prefoldin subunit 3

1

0

High-throughput

0.232

Low

836308

LTO1
Protein LTO1

1

0

High-throughput

0.232

Low

836373

TECR
Very-long-chain enoyl-CoA reductase

1

0

High-throughput

0.232

Low


Service provided by Combinatics, Japan

Last updated: 11Aug2025