Protein: YHI0
Putative proline--tRNA ligase YHR020W (EC 6.1.1.15) (Prolyl-tRNA synthetase) (ProRS)

Gene: YHI0, YHR020W
Organism: Saccharomyces cerevisiae S288C
Interactions: 207
UniProt ID: P38708

Gene Ontology (top 5):
GO:0002161 - aminoacyl-tRNA deacylase activity
GO:0004827 - proline-tRNA ligase activity
GO:0005524 - ATP binding
GO:0005737 - cytoplasm
GO:0006433 - prolyl-tRNA aminoacylation

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

416119

SYTC
Threonine--tRNA ligase, cytoplasmic

1

12

High-throughput

0.618

High

454165

SYH
Histidine--tRNA ligase, mitochondrial

1

1

High-throughput

0.618

High

793318

SYG
Glycine--tRNA ligase 1, mitochondrial

1

9

High-throughput

0.618

High

802538

SYNC
Asparagine--tRNA ligase, cytoplasmic

2

9

High-throughput

0.611

High

430840

ERF3
Eukaryotic peptide chain release factor GTP-binding subunit

2

1

Small-scale

0.584

High

558704

CAF20
Cap-associated protein CAF20

2

1

High-throughput

0.584

High

726995

HS104
Heat shock protein 104

2

4

High-throughput

0.584

High

802633

ATAD2
ATPase histone chaperone YTA7

2

7

High-throughput

0.571

High

536019

SEC7
ADP-ribosylation factor guanine nucleotide-exchange factor SEC7

2

1

High-throughput

0.567

High

802641

LCB2
Serine palmitoyltransferase 2

2

1

High-throughput

0.537

High

802670

BRE5
UBP3-associated protein BRE5

1

4

High-throughput

0.536

High

802680

UBP3
Ubiquitin carboxyl-terminal hydrolase 3

1

2

High-throughput

0.536

High

670665

SIS1
Protein SIS1

2

5

High-throughput

0.533

High

802645

RPN11
Ubiquitin carboxyl-terminal hydrolase RPN11

2

4

High-throughput

0.533

High

802647

SA155
SIT4-associating protein SAP155

2

1

High-throughput

0.533

High

531320

METK1
S-adenosylmethionine synthase 1

1

0

High-throughput

0.513

High

802632

RFC4
Replication factor C subunit 4

1

0

High-throughput

0.513

High

518009

SSBP1
Single-stranded nucleic acid-binding protein

1

1

High-throughput

0.501

High

441361

KAPB
cAMP-dependent protein kinase type 2

1

2

High-throughput

0.500

High

617389

GBB
Guanine nucleotide-binding protein subunit beta

1

4

High-throughput

0.500

High

644624

MDHC
Malate dehydrogenase, cytoplasmic

1

2

High-throughput

0.500

High

666176

MEK1
Meiosis-specific serine/threonine-protein kinase MEK1

1

1

High-throughput

0.500

High

675288

DMC1
Meiotic recombination protein DMC1

1

1

High-throughput

0.500

High

736091

ARP2
Actin-related protein 2

1

1

High-throughput

0.500

High

739505

DUR1
Urea amidolyase [Includes: Urea carboxylase

1

1

High-throughput

0.500

High

802683

UBC7
Ubiquitin-conjugating enzyme E2 7

1

3

High-throughput

0.500

High

797944

ATG12
Ubiquitin-like protein ATG12

1

1

High-throughput

0.489

High

802636

LYS12
Homoisocitrate dehydrogenase, mitochondrial

1

1

High-throughput

0.489

High

802696

UTP13
U3 small nucleolar RNA-associated protein 13

1

5

High-throughput

0.489

High

751391

CBF5
H/ACA ribonucleoprotein complex subunit CBF5

1

0

High-throughput

0.479

High

796072

IMB2
Importin subunit beta-2

1

0

High-throughput

0.479

High

149739

RS9A
Small ribosomal subunit protein uS4A

1

1

High-throughput

0.479

High

368895

SYMC
Methionine--tRNA ligase, cytoplasmic

1

11

High-throughput

0.479

High

392672

HSP82
ATP-dependent molecular chaperone HSP82

1

11

High-throughput

0.479

High

416231

SYDC
Aspartate--tRNA ligase, cytoplasmic

1

9

High-throughput

0.479

High

435063

RS3
Small ribosomal subunit protein uS3

1

3

High-throughput

0.479

High

438684

SC160
Protein SCP160

1

2

High-throughput

0.479

High

460339

SYV
Valine--tRNA ligase, mitochondrial

1

10

High-throughput

0.479

High

514616

RS18A
Small ribosomal subunit protein uS13A

1

2

High-throughput

0.479

High

560799

SYQ
Glutamine--tRNA ligase

1

6

High-throughput

0.479

High

579329

HSC82
ATP-dependent molecular chaperone HSC82

1

11

High-throughput

0.479

High

581856

SYKC
Lysine--tRNA ligase, cytoplasmic

1

9

High-throughput

0.479

High

597450

EF3A
Elongation factor 3A

1

7

High-throughput

0.479

High

657084

IF5A1
Eukaryotic translation initiation factor 5A-1

1

3

High-throughput

0.479

High

674597

RS2
Small ribosomal subunit protein uS5

1

4

High-throughput

0.479

High

679638

CDC48
Cell division control protein 48

1

5

High-throughput

0.479

High

682267

SPB4
ATP-dependent rRNA helicase SPB4

1

2

High-throughput

0.479

High

687586

SYLC
Leucine--tRNA ligase, cytoplasmic

1

12

High-throughput

0.479

High

713616

EF1G1
Elongation factor 1-gamma 1

1

4

High-throughput

0.479

High

737309

CDC12
Cell division control protein 12

1

1

High-throughput

0.479

High

739357

ZUO1
Zuotin

1

6

High-throughput

0.479

High

747831

SMY2
Protein SMY2

1

2

High-throughput

0.479

High

750646

PRS6A
26S proteasome regulatory subunit 6A

1

4

High-throughput

0.479

High

778304

RS27A
Small ribosomal subunit protein eS27A

1

1

High-throughput

0.479

High

779048

EF1G2
Elongation factor 1-gamma 2

1

4

High-throughput

0.479

High

783472

SYYC
Tyrosine--tRNA ligase, cytoplasmic

1

3

High-throughput

0.479

High

792022

GBLP
Small ribosomal subunit protein RACK1

1

4

High-throughput

0.479

High

795323

ATG8
Autophagy-related protein 8

1

3

High-throughput

0.479

High

796146

OLA1
Obg-like ATPase 1

1

2

High-throughput

0.479

High

802144

RS20
Small ribosomal subunit protein uS10

1

2

High-throughput

0.479

High

802614

RPN1
26S proteasome regulatory subunit RPN1

1

3

High-throughput

0.479

High

802615

SSZ1
Ribosome-associated complex subunit SSZ1

1

2

High-throughput

0.479

High

802622

DHH1
ATP-dependent RNA helicase DHH1

1

3

High-throughput

0.479

High

802624

RV167
Reduced viability upon starvation protein 167

1

6

High-throughput

0.479

High

802628

ARB1
ABC transporter ATP-binding protein ARB1

1

6

High-throughput

0.479

High

802635

RPAB4
DNA-directed RNA polymerases I, II, and III subunit RPABC4

1

1

High-throughput

0.479

High

802644

GCN20
Protein GCN20

1

6

High-throughput

0.479

High

802646

LSB3
LAS seventeen-binding protein 3

1

3

High-throughput

0.479

High

802648

SYEC
Glutamate--tRNA ligase, cytoplasmic

1

6

High-throughput

0.479

High

802649

ARC1
tRNA-aminoacylation cofactor ARC1

1

4

High-throughput

0.479

High

802652

MPS3
Spindle pole body assembly component MPS3

1

1

High-throughput

0.479

High

802656

RS12
Small ribosomal subunit protein eS12

1

1

High-throughput

0.479

High

802664

PIL1
Sphingolipid long chain base-responsive protein PIL1

1

2

High-throughput

0.479

High

802665

SPC98
Spindle pole body component SPC98

1

1

High-throughput

0.479

High

802668

HST2
NAD-dependent protein deacetylase HST2

1

2

High-throughput

0.479

High

802669

PPID
Peptidyl-prolyl cis-trans isomerase CPR6

1

1

High-throughput

0.479

High

802672

GIS2
Zinc finger protein GIS2

1

2

High-throughput

0.479

High

802673

PDR16
Phosphatidylinositol transfer protein PDR16

1

1

High-throughput

0.479

High

802682

RS15
Small ribosomal subunit protein uS19

1

1

High-throughput

0.479

High

802688

RLI1
Translation initiation factor RLI1

1

5

High-throughput

0.479

High

802693

MTQ2
eRF1 methyltransferase catalytic subunit MTQ2

1

1

High-throughput

0.479

High

802695

MYO5
Myosin-5

1

3

High-throughput

0.479

High

802700

IRC20
Uncharacterized ATP-dependent helicase IRC20

1

6

Small-scale

0.479

High

802706

NEW1
[NU+] prion formation protein 1

1

6

High-throughput

0.479

High

802707

KEL3
Kelch repeat-containing protein 3

1

1

High-throughput

0.479

High

802714

DBP10
ATP-dependent RNA helicase DBP10

1

6

High-throughput

0.479

High

394520

EF1A
Elongation factor 1-alpha

1

0

High-throughput

0.458

High

435938

RS13
Small ribosomal subunit protein uS15

1

0

High-throughput

0.458

High

780015

EAP1
Protein EAP1

1

0

High-throughput

0.458

High

797735

AIM4
Altered inheritance of mitochondria protein 4

1

0

High-throughput

0.458

High

802617

IF1A
Eukaryotic translation initiation factor 1A

1

0

High-throughput

0.458

High

802619

ATU2
Copper-transporting ATPase

1

0

High-throughput

0.458

High

802653

DENR
Translation machinery-associated protein 22

1

0

High-throughput

0.458

High

802678

TMA20
Translation machinery-associated protein 20

1

0

High-throughput

0.458

High

802686

ESP1
Separin

1

0

High-throughput

0.458

High

802692

FIN1
Filament protein FIN1

1

0

Small-scale

0.458

High

802697

BCP1
Protein BCP1

1

0

High-throughput

0.458

High

802702

LAM6
Membrane-anchored lipid-binding protein LAM6

1

0

High-throughput

0.458

High

802703

SGO1
Shugoshin

1

0

High-throughput

0.458

High

802710

MLH3
DNA mismatch repair protein MLH3

1

0

High-throughput

0.458

High

802712

MDY2
Ubiquitin-like protein MDY2

1

0

High-throughput

0.458

High

575336

EI2BA
Translation initiation factor eIF2B subunit alpha

1

1

High-throughput

0.457

High

738634

EI2BB
Translation initiation factor eIF2B subunit beta

1

1

High-throughput

0.457

High

793913

TAF5
SAGA complex/transcription factor TFIID complex subunit TAF5

1

4

High-throughput

0.457

High

802640

SA185
SIT4-associating protein SAP185

1

1

High-throughput

0.457

High

802642

COPB2
Coatomer subunit beta'

1

3

High-throughput

0.457

High

802667

COPA
Coatomer subunit alpha

1

3

High-throughput

0.457

High

526596

HSP72
Heat shock protein SSA2

1

3

High-throughput

0.445

High

544735

SSB1
Ribosome-associated molecular chaperone SSB1

1

5

High-throughput

0.445

High

646125

NPR1
Nitrogen permease reactivator protein

1

7

High-throughput

0.445

High

802613

DBP8
ATP-dependent RNA helicase DBP8

1

1

High-throughput

0.445

High

802643

EMW1
Essential for maintenance of the cell wall protein 1

1

4

High-throughput

0.445

High

802666

MPA43
Protein MPA43

1

1

High-throughput

0.445

High

802671

HCH1
Hsp90 co-chaperone HCH1

1

2

High-throughput

0.445

High

802685

PDR12
ATP-dependent permease PDR12

1

1

High-throughput

0.445

High

802694

PDR15
ATP-dependent permease PDR15

1

1

High-throughput

0.445

High

802701

SSK1
Osmolarity two-component system protein SSK1

1

1

High-throughput

0.445

High

802715

RUVB2
RuvB-like protein 2

1

2

High-throughput

0.445

High

802718

DUF1
DUB-associated factor 1

1

6

High-throughput

0.445

High

433618

RL26A
Large ribosomal subunit protein uL24A

1

1

High-throughput

0.437

High

441884

RS14A
Small ribosomal subunit protein uS11A

1

1

High-throughput

0.437

High

452021

TOM70
Mitochondrial import receptor subunit TOM70

1

9

High-throughput

0.437

High

503019

RS22A
Small ribosomal subunit protein uS8A

1

1

High-throughput

0.437

High

619161

IF5A2
Eukaryotic translation initiation factor 5A-2

1

3

High-throughput

0.437

High

647097

RPC2
DNA-directed RNA polymerase III subunit RPC2

1

1

High-throughput

0.437

High

706996

TF2B
Transcription initiation factor IIB

1

2

High-throughput

0.437

High

736379

MET4
Transcriptional activator of sulfur metabolism MET4

1

1

High-throughput

0.437

High

739623

SPT16
FACT complex subunit SPT16

1

2

High-throughput

0.437

High

747339

RSSA1
Small ribosomal subunit protein uS2A

1

3

High-throughput

0.437

High

748606

SRPR
Signal recognition particle receptor subunit alpha homolog

1

1

High-throughput

0.437

High

799742

IF5
Eukaryotic translation initiation factor 5

1

2

High-throughput

0.437

High

802616

RPN10
26S proteasome regulatory subunit RPN10

1

2

High-throughput

0.437

High

802618

SEC31
Protein transport protein SEC31

1

3

High-throughput

0.437

High

802621

CAJ1
Protein CAJ1

1

6

High-throughput

0.437

High

802623

LAA1
AP-1 accessory protein LAA1

1

2

High-throughput

0.437

High

802626

SIT1
Siderophore iron transporter 1

1

3

High-throughput

0.437

High

802638

DSN1
Kinetochore-associated protein DSN1

1

1

High-throughput

0.437

High

802655

HOSC
Homocitrate synthase, cytosolic isozyme

1

2

High-throughput

0.437

High

802657

VATG
V-type proton ATPase subunit G

1

1

High-throughput

0.437

High

802663

EIF2A
Eukaryotic translation initiation factor 2A

1

1

High-throughput

0.437

High

802676

SAM50
Sorting assembly machinery 50 kDa subunit

1

1

High-throughput

0.437

High

802679

NUM1
Nuclear migration protein NUM1

1

2

High-throughput

0.437

High

802681

MAP1
Methionine aminopeptidase 1

1

1

High-throughput

0.437

High

802691

YMC0
Uncharacterized protein YML020W

1

1

High-throughput

0.437

High

802698

TFC6
Transcription factor tau 91 kDa subunit

1

1

High-throughput

0.437

High

802704

RS10A
Small ribosomal subunit protein eS10A

1

2

High-throughput

0.437

High

802705

AP3D
AP-3 complex subunit delta

1

2

High-throughput

0.437

High

802708

TRMB
tRNA

1

1

High-throughput

0.437

High

802711

RRP6
Exosome complex exonuclease RRP6

1

3

High-throughput

0.437

High

632255

RPAB2
DNA-directed RNA polymerases I, II, and III subunit RPABC2

1

0

High-throughput

0.426

High

781926

KAE1
tRNA N6-adenosine threonylcarbamoyltransferase

1

0

High-throughput

0.426

High

782202

GPT2
Glycerol-3-phosphate O-acyltransferase 2

1

0

High-throughput

0.426

High

802625

SLN1
Osmosensing histidine protein kinase SLN1

1

0

High-throughput

0.426

High

802629

MGDP1
Putative magnesium-dependent phosphatase YER134C

1

0

High-throughput

0.426

High

802630

VID27
Vacuolar import and degradation protein 27

1

0

High-throughput

0.426

High

802661

ARI1
NADPH-dependent aldehyde reductase ARI1

1

0

High-throughput

0.426

High

802674

PFD4
Prefoldin subunit 4

1

0

High-throughput

0.426

High

802675

SIW14
Inositol diphosphatase SIW14

1

0

High-throughput

0.426

High

802687

FDC1
Ferulic acid decarboxylase 1

1

0

High-throughput

0.426

High

802689

YM44
Uncharacterized protein YMR178W

1

0

High-throughput

0.426

High

456394

ILVB
Acetolactate synthase catalytic subunit, mitochondrial

1

1

High-throughput

0.407

High

659332

MRS4
Mitochondrial RNA-splicing protein MRS4

1

1

High-throughput

0.407

High

703814

LAC1
Ceramide synthase LAC1

1

1

High-throughput

0.407

High

737287

HXT4
Low-affinity glucose transporter HXT4

1

1

High-throughput

0.407

High

802620

HXT7
High-affinity hexose transporter HXT7

1

1

High-throughput

0.407

High

802631

ELO3
Fatty acid elongase 3

1

1

High-throughput

0.407

High

802658

PHB2
Prohibitin-2

1

2

High-throughput

0.407

High

802716

NOP58
Nucleolar protein 58

1

1

High-throughput

0.407

High

748167

SUI1
Eukaryotic translation initiation factor eIF-1

2

0

High-throughput

0.310

High

793259

TAT1
Valine/tyrosine/tryptophan amino-acid permease 1

2

0

High-throughput

0.307

High

550576

SST2
Protein SST2

1

0

High-throughput

0.279

Low

600685

SEC2
Rab guanine nucleotide exchange factor SEC2

1

0

High-throughput

0.279

Low

737564

ERG1
Squalene epoxidase ERG1

1

0

High-throughput

0.279

Low

459083

RPAC1
DNA-directed RNA polymerases I and III subunit RPAC1

1

0

High-throughput

0.261

Low

383678

RS17A
Small ribosomal subunit protein eS17A

1

0

High-throughput

0.249

Low

410107

RL25
Large ribosomal subunit protein uL23

1

0

High-throughput

0.249

Low

455038

RS19A
Small ribosomal subunit protein eS19A

1

0

High-throughput

0.249

Low

504189

RL31A
Large ribosomal subunit protein eL31A

1

0

High-throughput

0.249

Low

512417

RS6A
Small ribosomal subunit protein eS6A

1

0

High-throughput

0.249

Low

512985

RS8A
Small ribosomal subunit protein eS8A

1

0

High-throughput

0.249

Low

584911

TRM1
tRNA

1

0

High-throughput

0.249

Low

689430

RS7A
Small ribosomal subunit protein eS7A

1

0

High-throughput

0.249

Low

753347

RS3A1
Small ribosomal subunit protein eS1A

1

0

High-throughput

0.249

Low

757649

IF4B
Eukaryotic translation initiation factor 4B

1

0

High-throughput

0.249

Low

781179

RL14A
Large ribosomal subunit protein eL14A

1

0

High-throughput

0.249

Low

802627

IRC22
Increased recombination centers protein 22

1

0

High-throughput

0.249

Low

802639

RLP7
Ribosome biogenesis protein RLP7

1

0

High-throughput

0.249

Low

802677

SEC5
Exocyst complex component SEC5

1

0

High-throughput

0.249

Low

802699

URN1
Pre-mRNA-splicing factor URN1

1

0

High-throughput

0.249

Low

554342

HMDH1
3-hydroxy-3-methylglutaryl-coenzyme A reductase 1

1

0

High-throughput

0.232

Low

613852

ERD2
ER lumen protein-retaining receptor

1

0

High-throughput

0.232

Low

636306

ACO1
Acyl-CoA desaturase 1

1

0

High-throughput

0.232

Low

646729

SEC15
Exocyst complex component SEC15

1

0

High-throughput

0.232

Low

780099

MIA40
Mitochondrial intermembrane space import and assembly protein 40

1

0

High-throughput

0.232

Low

802634

NTA1
Protein N-terminal amidase

1

0

High-throughput

0.232

Low

802637

ICE2
Protein ICE2

1

0

High-throughput

0.232

Low

802650

PHO86
Inorganic phosphate transporter PHO86

1

0

High-throughput

0.232

Low

802651

NU192
Nucleoporin NUP192

1

0

High-throughput

0.232

Low

802654

SEC16
COPII coat assembly protein SEC16

1

0

High-throughput

0.232

Low

802659

NU188
Nucleoporin NUP188

1

0

High-throughput

0.232

Low

802660

ERG25
C-4 methylsterol oxidase ERG25

1

0

High-throughput

0.232

Low

802662

YG1I
Uncharacterized membrane protein YGR026W

1

0

High-throughput

0.232

Low

802684

TIM50
Mitochondrial import inner membrane translocase subunit TIM50

1

0

High-throughput

0.232

Low

802690

SCS7
Ceramide very long chain fatty acid hydroxylase SCS7

1

0

High-throughput

0.232

Low

802709

FSF1
Sideroflexin FSF1

1

0

High-throughput

0.232

Low

802713

MAM3
Protein MAM3

1

0

High-throughput

0.232

Low

802717

TECR
Very-long-chain enoyl-CoA reductase

1

0

High-throughput

0.232

Low


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Last updated: 11Aug2025