Protein: ESP1
Separin (EC 3.4.22.49) (Separase)

Gene: ESP1
Organism: Saccharomyces cerevisiae S288C
Interactions: 170
UniProt ID: Q03018

Gene Ontology (top 5):
GO:0000070 - mitotic sister chromatid segregation
GO:0004197 - cysteine-type endopeptidase activity
GO:0005634 - nucleus
GO:0005737 - cytoplasm
GO:0005739 - mitochondrion

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

822237

SECU
Securin

12

0

Small-scale

0.930

High

1040555

SLK19
Kinetochore protein SLK19

2

1

Small-scale

0.601

High

1040559

SCC1
Sister chromatid cohesion protein 1

2

2

Small-scale

0.595

High

1024981

2ABA
Protein phosphatase PP2A regulatory subunit B

2

0

Small-scale

0.576

High

919350

RAGCD
GTP-binding protein GTR2

2

0

High-throughput

0.563

High

740003

CDC5
Cell cycle serine/threonine-protein kinase CDC5/MSD2

1

1

Small-scale

0.515

High

893431

ZDS1
Protein ZDS1

1

1

Small-scale

0.515

High

814260

RV167
Reduced viability upon starvation protein 167

1

1

Small-scale

0.508

High

660877

PP2A2
Serine/threonine-protein phosphatase PP2A-2 catalytic subunit

1

2

Small-scale

0.501

High

744623

TFB1
General transcription and DNA repair factor IIH subunit TFB1

1

0

High-throughput

0.493

High

364708

CDK1
Cyclin-dependent kinase 1

1

0

Small-scale

0.480

High

368189

THDH
Threonine dehydratase, mitochondrial

1

1

High-throughput

0.479

High

368920

SYMC
Methionine--tRNA ligase, cytoplasmic

1

1

High-throughput

0.479

High

402100

RPB1
DNA-directed RNA polymerase II subunit RPB1

1

1

High-throughput

0.479

High

416277

SYDC
Aspartate--tRNA ligase, cytoplasmic

1

1

High-throughput

0.479

High

439591

LEU1
2-isopropylmalate synthase

1

1

High-throughput

0.479

High

444608

DED1
ATP-dependent RNA helicase DED1

1

1

High-throughput

0.479

High

534304

RPA1
DNA-directed RNA polymerase I subunit RPA190

1

1

High-throughput

0.479

High

581895

SYKC
Lysine--tRNA ligase, cytoplasmic

1

1

High-throughput

0.479

High

597253

BIP
Endoplasmic reticulum chaperone BiP

1

1

High-throughput

0.479

High

632725

DBP4
ATP-dependent RNA helicase HCA4

1

1

High-throughput

0.479

High

661190

SPT6
Transcription elongation factor SPT6

1

1

High-throughput

0.479

High

666599

DBP1
ATP-dependent RNA helicase DBP1

1

1

High-throughput

0.479

High

678445

PWP2
Periodic tryptophan protein 2

1

1

High-throughput

0.479

High

687611

SYLC
Leucine--tRNA ligase, cytoplasmic

1

2

High-throughput

0.479

High

722203

XPO1
Exportin-1

1

1

High-throughput

0.479

High

739449

ZUO1
Zuotin

1

3

High-throughput

0.479

High

740211

RPN2
26S proteasome regulatory subunit RPN2

1

1

High-throughput

0.479

High

750997

PRS7
26S proteasome regulatory subunit 7 homolog

1

1

High-throughput

0.479

High

751114

PDR5
Pleiotropic ABC efflux transporter of multiple drugs

1

2

High-throughput

0.479

High

755156

GCN1
eIF-2-alpha kinase activator GCN1

1

3

High-throughput

0.479

High

785964

LONM
Lon protease homolog, mitochondrial

1

1

High-throughput

0.479

High

800394

YPT31
GTP-binding protein YPT31/YPT8

1

1

High-throughput

0.479

High

802970

6PGD1
6-phosphogluconate dehydrogenase, decarboxylating 1

1

1

High-throughput

0.479

High

803663

RPN1
26S proteasome regulatory subunit RPN1

1

1

High-throughput

0.479

High

808777

NAB3
Nuclear polyadenylated RNA-binding protein 3

1

1

High-throughput

0.479

High

817283

ARB1
ABC transporter ATP-binding protein ARB1

1

2

High-throughput

0.479

High

864505

MTR4
ATP-dependent RNA helicase DOB1

1

1

High-throughput

0.479

High

1040554

SUB2
ATP-dependent RNA helicase SUB2

1

1

High-throughput

0.479

High

1040556

NEW1
[NU+] prion formation protein 1

1

3

High-throughput

0.479

High

1040557

MDN1
Midasin

1

1

High-throughput

0.479

High

1040561

LEU9
2-isopropylmalate synthase 2, mitochondrial

1

1

High-throughput

0.479

High

1040565

GLT1
Glutamate synthase [NADH]

1

1

High-throughput

0.479

High

617813

SNF6
Transcription regulatory protein SNF6

1

0

High-throughput

0.460

High

368250

TRP
Tryptophan synthase

1

0

High-throughput

0.458

High

432356

METE
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

1

0

High-throughput

0.458

High

438824

CYSD
Homocysteine/cysteine synthase

1

0

High-throughput

0.458

High

452528

PUR2
Bifunctional purine biosynthetic protein ADE5,7 [Includes: Phosphoribosylamine--glycine ligase

1

0

High-throughput

0.458

High

456447

ILVB
Acetolactate synthase catalytic subunit, mitochondrial

1

0

High-throughput

0.458

High

470581

RPB2
DNA-directed RNA polymerase II subunit RPB2

1

0

High-throughput

0.458

High

471429

ARO1
Pentafunctional AROM polypeptide [Includes: 3-dehydroquinate synthase

1

0

High-throughput

0.458

High

482082

C1TM
C-1-tetrahydrofolate synthase, mitochondrial

1

0

High-throughput

0.458

High

533527

AK
Aspartokinase

1

0

High-throughput

0.458

High

542733

G6PD
Glucose-6-phosphate 1-dehydrogenase

1

0

High-throughput

0.458

High

553646

ERF1
Eukaryotic peptide chain release factor subunit 1

1

0

High-throughput

0.458

High

555139

G6PI
Glucose-6-phosphate isomerase

1

0

High-throughput

0.458

High

560817

SYQ
Glutamine--tRNA ligase

1

0

High-throughput

0.458

High

566518

DHAS
Aspartate-semialdehyde dehydrogenase

1

0

High-throughput

0.458

High

594990

ODPA
Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial

1

0

High-throughput

0.458

High

598431

TUP1
General transcriptional corepressor TUP1

1

0

High-throughput

0.458

High

607785

HXKG
Glucokinase-1

1

0

High-throughput

0.458

High

632819

DBP5
ATP-dependent RNA helicase DBP5

1

0

High-throughput

0.458

High

637271

PWP1
Periodic tryptophan protein 1

1

0

High-throughput

0.458

High

638734

RIR1
Ribonucleoside-diphosphate reductase large chain 1

1

0

High-throughput

0.458

High

639056

VPS1
Vacuolar protein sorting-associated protein 1

1

0

High-throughput

0.458

High

642452

MCK1
Protein kinase MCK1

1

0

High-throughput

0.458

High

645516

XRN1
5'-3' exoribonuclease 1

1

0

High-throughput

0.458

High

649518

UBA1
Ubiquitin-activating enzyme E1 1

1

0

High-throughput

0.458

High

684825

RL5
Large ribosomal subunit protein uL18

1

0

High-throughput

0.458

High

701639

PYRD
Dihydroorotate dehydrogenase

1

0

High-throughput

0.458

High

701736

URA7
CTP synthase 1

1

0

High-throughput

0.458

High

713513

TRXB1
Thioredoxin reductase 1

1

0

High-throughput

0.458

High

715427

MPI
Mannose-6-phosphate isomerase

1

0

High-throughput

0.458

High

721409

MCM4
DNA replication licensing factor MCM4

1

0

High-throughput

0.458

High

721659

RENT1
ATP-dependent helicase NAM7

1

0

High-throughput

0.458

High

734382

OAC1
Mitochondrial oxaloacetate transport protein

1

0

High-throughput

0.458

High

737487

ODPB
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial

1

0

High-throughput

0.458

High

738605

EI2BE
Translation initiation factor eIF2B subunit epsilon

1

0

High-throughput

0.458

High

739090

PAN1
Actin cytoskeleton-regulatory complex protein PAN1

1

0

High-throughput

0.458

High

740281

SMI1
Cell wall assembly regulator SMI1

1

0

High-throughput

0.458

High

743355

FIMB
Fimbrin

1

0

High-throughput

0.458

High

750135

MRT4
Ribosome assembly factor MRT4

1

0

High-throughput

0.458

High

757525

NCBP1
Nuclear cap-binding protein complex subunit 1

1

0

High-throughput

0.458

High

779440

MAOM
NAD-dependent malic enzyme, mitochondrial

1

0

High-throughput

0.458

High

783504

SYYC
Tyrosine--tRNA ligase, cytoplasmic

1

0

High-throughput

0.458

High

790222

GLYC
Serine hydroxymethyltransferase, cytosolic

1

0

High-throughput

0.458

High

791098

NOP4
Nucleolar protein 4

1

0

High-throughput

0.458

High

791918

AAP1
Alanine/arginine aminopeptidase

1

0

High-throughput

0.458

High

793105

ATPG
ATP synthase subunit gamma, mitochondrial

1

0

High-throughput

0.458

High

793562

ADH5
Alcohol dehydrogenase 5

1

0

High-throughput

0.458

High

796618

EIF3A
Eukaryotic translation initiation factor 3 subunit A

1

0

High-throughput

0.458

High

800913

NCB5R
NADH-cytochrome b5 reductase 1

1

0

High-throughput

0.458

High

801100

FKS1
1,3-beta-glucan synthase component FKS1

1

0

High-throughput

0.458

High

802686

YHI0
Putative proline--tRNA ligase YHR020W

1

0

High-throughput

0.458

High

805869

ARO9
Aromatic amino acid aminotransferase 2

1

0

High-throughput

0.458

High

808440

PUR4
Phosphoribosylformylglycinamidine synthase

1

0

High-throughput

0.458

High

815220

IF4F1
Eukaryotic initiation factor 4F subunit p150

1

0

High-throughput

0.458

High

815547

RSP5
E3 ubiquitin-protein ligase RSP5

1

0

High-throughput

0.458

High

816209

SPF1
Endoplasmic reticulum transmembrane helix translocase

1

0

High-throughput

0.458

High

818695

GPP2
Glycerol-1-phosphate phosphohydrolase 2

1

0

High-throughput

0.458

High

820805

CCS1
Superoxide dismutase 1 copper chaperone

1

0

High-throughput

0.458

High

825976

GVP36
Protein GVP36

1

0

High-throughput

0.458

High

828275

SYA
Alanine--tRNA ligase, mitochondrial

1

0

High-throughput

0.458

High

881819

LYS4
Homoaconitase, mitochondrial

1

0

High-throughput

0.458

High

915246

ACS2
Acetyl-coenzyme A synthetase 2

1

0

High-throughput

0.458

High

917312

INO80
Chromatin-remodeling ATPase INO80

1

0

High-throughput

0.458

High

917464

PRP43
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43

1

0

High-throughput

0.458

High

924014

PDR16
Phosphatidylinositol transfer protein PDR16

1

0

High-throughput

0.458

High

924400

RIA1
Ribosome assembly protein 1

1

0

High-throughput

0.458

High

1009781

PURA
Adenylosuccinate synthetase

1

0

High-throughput

0.458

High

1028022

ACAC
Acetyl-CoA carboxylase

1

0

High-throughput

0.458

High

1040546

OYE2
NADPH dehydrogenase 2

1

0

High-throughput

0.458

High

1040547

CLU
Clustered mitochondria protein 1

1

0

High-throughput

0.458

High

1040548

ARX1
Probable metalloprotease ARX1

1

0

High-throughput

0.458

High

1040549

YHM2
Citrate/oxoglutarate carrier protein

1

0

High-throughput

0.458

High

1040552

SYRC
Arginine--tRNA ligase, cytoplasmic

1

0

High-throughput

0.458

High

1040553

ELP1
Elongator complex protein 1

1

0

High-throughput

0.458

High

1040560

YRA1
RNA annealing protein YRA1

1

0

High-throughput

0.458

High

1040562

RPN5
26S proteasome regulatory subunit RPN5

1

0

High-throughput

0.458

High

1040563

IQG1
Ras GTPase-activating-like protein IQG1

1

0

High-throughput

0.458

High

1040566

SEY1
Protein SEY1

1

0

High-throughput

0.458

High

524941

HSP71
Heat shock protein SSA1

1

1

High-throughput

0.445

High

546019

SSB1
Ribosome-associated molecular chaperone SSB1

1

1

High-throughput

0.445

High

676288

MAS5
Mitochondrial protein import protein MAS5

1

1

High-throughput

0.445

High

804402

SSZ1
Ribosome-associated complex subunit SSZ1

1

1

High-throughput

0.445

High

810751

TCPB
T-complex protein 1 subunit beta

1

2

High-throughput

0.445

High

481912

SYIC
Isoleucine--tRNA ligase, cytoplasmic

1

1

High-throughput

0.437

High

554729

YPK1
Serine/threonine-protein kinase YPK1

1

1

High-throughput

0.437

High

582511

SEC23
Protein transport protein SEC23

1

1

High-throughput

0.437

High

616447

SEC18
Vesicular-fusion protein SEC18

1

1

High-throughput

0.437

High

672339

RV161
Reduced viability upon starvation protein 161

1

1

High-throughput

0.437

High

799765

IF5
Eukaryotic translation initiation factor 5

1

1

High-throughput

0.437

High

805169

SFB3
SED5-binding protein 3

1

2

High-throughput

0.437

High

811024

TCPD
T-complex protein 1 subunit delta

1

2

High-throughput

0.437

High

823638

TCPE
T-complex protein 1 subunit epsilon

1

2

High-throughput

0.437

High

825242

SEC24
Protein transport protein SEC24

1

2

High-throughput

0.437

High

836088

COPB2
Coatomer subunit beta'

1

1

High-throughput

0.437

High

849548

GCN20
Protein GCN20

1

2

High-throughput

0.437

High

850339

UBP6
Ubiquitin carboxyl-terminal hydrolase 6

1

3

High-throughput

0.437

High

909762

YPT32
GTP-binding protein YPT32/YPT11

1

1

High-throughput

0.437

High

919613

6PGD2
6-phosphogluconate dehydrogenase, decarboxylating 2

1

1

High-throughput

0.437

High

922108

COPA
Coatomer subunit alpha

1

1

High-throughput

0.437

High

1036912

MLP1
Protein MLP1

1

1

High-throughput

0.437

High

1038590

PDR12
ATP-dependent permease PDR12

1

2

High-throughput

0.437

High

1040550

PDR15
ATP-dependent permease PDR15

1

2

High-throughput

0.437

High

393218

HSP82
ATP-dependent molecular chaperone HSP82

1

0

High-throughput

0.426

High

912435

PFD6
Prefoldin subunit 6

1

0

High-throughput

0.426

High

739742

SPT16
FACT complex subunit SPT16

2

0

High-throughput

0.305

High

506513

YL016
Putative truncated guanine nucleotide exchange factor SDC25

1

0

High-throughput

0.261

Low

383186

RLA4
Large ribosomal subunit protein P2B

1

0

High-throughput

0.249

Low

405133

LEU3
3-isopropylmalate dehydrogenase

1

0

High-throughput

0.249

Low

438480

EIF3B
Eukaryotic translation initiation factor 3 subunit B

1

0

High-throughput

0.249

Low

513757

RL2A
Large ribosomal subunit protein uL2A

1

0

High-throughput

0.249

Low

536046

SEC7
ADP-ribosylation factor guanine nucleotide-exchange factor SEC7

1

0

High-throughput

0.249

Low

575505

GFA1
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

1

0

High-throughput

0.249

Low

644828

CLH
Clathrin heavy chain

1

0

High-throughput

0.249

Low

671384

PHO84
Inorganic phosphate transporter PHO84

1

0

High-throughput

0.249

Low

673673

USO1
Intracellular protein transport protein USO1

1

0

High-throughput

0.249

Low

732185

COPG
Coatomer subunit gamma

1

0

High-throughput

0.249

Low

735752

VA0D
V-type proton ATPase subunit d

1

0

High-throughput

0.249

Low

737587

ERG1
Squalene epoxidase ERG1

1

0

High-throughput

0.249

Low

737764

IF2G
Eukaryotic translation initiation factor 2 subunit gamma

1

0

High-throughput

0.249

Low

756656

NIC96
Nucleoporin NIC96

1

0

High-throughput

0.249

Low

790428

GLY1
Low specificity L-threonine aldolase

1

0

High-throughput

0.249

Low

836020

COPB
Coatomer subunit beta

1

0

High-throughput

0.249

Low

849971

VTC2
Vacuolar transporter chaperone complex subunit 2

1

0

High-throughput

0.249

Low

924615

NAT10
RNA cytidine acetyltransferase

1

0

High-throughput

0.249

Low

1040551

RRP5
rRNA biogenesis protein RRP5

1

0

High-throughput

0.249

Low

1040558

GET3
ATPase GET3

1

0

High-throughput

0.249

Low

1040564

ERG27
3-keto-steroid reductase ERG27

1

0

High-throughput

0.249

Low


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Last updated: 11Aug2025