Protein: RFA1
Replication factor A protein 1 (RF-A protein 1) (DNA-binding protein BUF2) (Replication protein A 69 kDa DNA-binding subunit) (Single-stranded DNA-binding protein)

Gene: RFA1
Organism: Saccharomyces cerevisiae S288C
Interactions: 230
UniProt ID: P22336

Gene Ontology (top 5):
GO:0000722 - telomere maintenance via recombination
GO:0000723 - telomere maintenance
GO:0000724 - double-strand break repair via homologous recombination
GO:0000781 - chromosome, telomeric region
GO:0000794 - condensed nuclear chromosome

Download interactions: TXT   MITAB-2.5

Interaction Interactor Experiments Homologs Category Interaction
Score
Confidence

647711

RFA2
Replication factor A protein 2

17

9

Small-scale

0.986

High

647712

RFA3
Replication factor A protein 3

18

0

Small-scale

0.935

High

647754

ATR
Serine/threonine-protein kinase MEC1

12

8

Small-scale

0.761

High

448429

RAD52
DNA repair and recombination protein RAD52

12

4

Small-scale

0.759

High

647766

DNA2
DNA replication ATP-dependent helicase/nuclease DNA2 [Includes: DNA replication nuclease DNA2

11

3

Small-scale

0.757

High

647694

RFA1
Replication factor A protein 1

2

3

Small-scale

0.754

High

647782

MPH1
ATP-dependent DNA helicase MPH1

8

10

Small-scale

0.747

High

647816

H3
Histone H3

6

6

Small-scale

0.732

High

647705

RAD51
DNA repair protein RAD51

5

3

Small-scale

0.714

High

647840

IMB1
Importin subunit beta-1

5

7

Small-scale

0.712

High

647745

SGS1
ATP-dependent helicase SGS1

4

13

Small-scale

0.710

High

647832

LCD1
DNA damage checkpoint protein LCD1

6

0

Small-scale

0.705

High

647773

RT105
Regulator of Ty1 transposition protein 105

5

0

Small-scale

0.698

High

647717

MCM2
DNA replication licensing factor MCM2

4

7

Small-scale

0.676

High

647709

MSH2
DNA mismatch repair protein MSH2

3

5

Small-scale

0.662

High

647828

MSH6
DNA mismatch repair protein MSH6

3

5

Small-scale

0.662

High

381406

H2B2
Histone H2B.2

3

4

Small-scale

0.650

High

381881

H4
Histone H4

3

1

Small-scale

0.640

High

392246

HSP82
ATP-dependent molecular chaperone HSP82

3

3

High-throughput

0.640

High

647836

POB3
FACT complex subunit POB3

2

1

Small-scale

0.634

High

454513

PIF1
ATP-dependent DNA helicase PIF1

2

1

Small-scale

0.624

High

647727

SPT16
FACT complex subunit SPT16

2

4

Small-scale

0.624

High

647846

BRE1
E3 ubiquitin-protein ligase BRE1

2

9

Small-scale

0.624

High

365562

PGK
Phosphoglycerate kinase

2

1

High-throughput

0.621

High

647827

SLD5
DNA replication complex GINS protein SLD5

2

1

Small-scale

0.611

High

647830

RUVB1
RuvB-like protein 1

2

3

Small-scale

0.611

High

647869

CMR1
DNA damage-binding protein CMR1

2

6

High-throughput

0.611

High

647731

FIMB
Fimbrin

2

9

Small-scale

0.606

High

647696

BUR1
Serine/threonine-protein kinase BUR1

2

7

Small-scale

0.601

High

647697

MCM3
DNA replication licensing factor MCM3

2

7

Small-scale

0.595

High

647856

SIZ2
E3 SUMO-protein ligase SIZ2

2

1

Small-scale

0.595

High

547851

NHP6B
Non-histone chromosomal protein 6B

2

8

High-throughput

0.588

High

647771

HSP7E
Heat shock protein SSC3, mitochondrial

2

6

High-throughput

0.588

High

647837

RNH1
Ribonuclease H

2

1

High-throughput

0.588

High

380973

H2B1
Histone H2B.1

2

4

Small-scale

0.584

High

419603

H2A2
Histone H2A.2

2

4

Small-scale

0.584

High

448359

RAD1
DNA repair protein RAD1

2

2

Small-scale

0.584

High

647805

INO80
Chromatin-remodeling ATPase INO80

2

12

Small-scale

0.584

High

647819

CTF4
DNA polymerase alpha-binding protein

2

7

Small-scale

0.584

High

647847

CDC45
Cell division control protein 45

2

1

Small-scale

0.584

High

647851

ISW2
ISWI chromatin-remodeling complex ATPase ISW2

2

11

Small-scale

0.584

High

533469

RAD18
Postreplication repair E3 ubiquitin-protein ligase RAD18

1

3

Small-scale

0.573

High

578867

HSC82
ATP-dependent molecular chaperone HSC82

2

3

High-throughput

0.571

High

647702

SIS1
Protein SIS1

2

5

High-throughput

0.571

High

647695

HSP12
12 kDa heat shock protein

2

0

High-throughput

0.563

High

647748

BDF1
Bromodomain-containing factor 1

1

5

Small-scale

0.561

High

590534

HSP26
Heat shock protein 26

2

0

High-throughput

0.559

High

647723

RIM1
Single-stranded DNA-binding protein RIM1, mitochondrial

2

0

Small-scale

0.559

High

647784

DSN1
Kinetochore-associated protein DSN1

2

0

High-throughput

0.559

High

647775

SSB2
Ribosome-associated molecular chaperone SSB2

2

3

High-throughput

0.552

High

622319

ACON
Aconitate hydratase, mitochondrial

1

1

High-throughput

0.536

High

647704

MSH3
DNA mismatch repair protein MSH3

1

5

High-throughput

0.536

High

647710

CDC20
APC/C activator protein CDC20

1

4

High-throughput

0.536

High

647752

GBLP
Small ribosomal subunit protein RACK1

1

6

High-throughput

0.536

High

647772

ICP55
Intermediate cleaving peptidase 55

1

3

High-throughput

0.536

High

647776

PRS4
26S proteasome regulatory subunit 4 homolog

1

5

High-throughput

0.536

High

647810

BRE5
UBP3-associated protein BRE5

1

2

High-throughput

0.536

High

647820

UBP3
Ubiquitin carboxyl-terminal hydrolase 3

1

6

High-throughput

0.536

High

647833

KU80
ATP-dependent DNA helicase II subunit 2

1

5

Small-scale

0.534

High

647737

XRS2
DNA repair protein XRS2

1

0

Small-scale

0.529

High

555228

RAD50
DNA repair protein RAD50

1

6

Small-scale

0.527

High

401890

RPB1
DNA-directed RNA polymerase II subunit RPB1

1

3

High-throughput

0.515

High

647724

ASF1
Histone chaperone ASF1

1

1

High-throughput

0.515

High

647831

HMO1
High mobility group protein 1

1

8

High-throughput

0.515

High

647841

TERT
Telomerase reverse transcriptase

1

1

Small-scale

0.515

High

647803

MDM34
Mitochondrial distribution and morphology protein 34

1

0

Small-scale

0.513

High

647835

DPB4
DNA polymerase epsilon subunit D

1

0

High-throughput

0.513

High

647713

FEN1
Flap endonuclease 1

1

2

Small-scale

0.501

High

647753

ATM
Serine/threonine-protein kinase TEL1

1

7

Small-scale

0.501

High

647797

DPB11
DNA replication regulator DPB11

1

1

Small-scale

0.501

High

647850

ESA1
Histone acetyltransferase ESA1

1

2

Small-scale

0.501

High

368593

PMG1
Phosphoglycerate mutase 1

1

3

High-throughput

0.500

High

386573

TBB
Tubulin beta chain

1

3

High-throughput

0.500

High

425107

OTC
Ornithine carbamoyltransferase

1

2

High-throughput

0.500

High

452820

ATPA
ATP synthase subunit alpha, mitochondrial

1

4

High-throughput

0.500

High

453426

PYR1
Multifunctional protein URA2

1

4

High-throughput

0.500

High

486038

TBA1
Tubulin alpha-1 chain

1

5

High-throughput

0.500

High

486407

TBA3
Tubulin alpha-3 chain

1

5

High-throughput

0.500

High

555009

G6PI
Glucose-6-phosphate isomerase

1

1

High-throughput

0.500

High

592019

VATB
V-type proton ATPase subunit B

1

4

High-throughput

0.500

High

597041

BIP
Endoplasmic reticulum chaperone BiP

1

3

High-throughput

0.500

High

644713

CLH
Clathrin heavy chain

1

2

High-throughput

0.500

High

647703

SCJ1
DnaJ-related protein SCJ1

1

4

High-throughput

0.500

High

647815

ACT
Actin

1

4

High-throughput

0.500

High

647821

PRS8
26S proteasome regulatory subunit 8 homolog

1

6

High-throughput

0.500

High

647849

RTC1
Restriction of telomere capping protein 1

1

4

High-throughput

0.500

High

647734

CDC13
Cell division control protein 13

1

0

Small-scale

0.493

High

647722

ARP2
Actin-related protein 2

1

5

High-throughput

0.489

High

358117

ADH1
Alcohol dehydrogenase 1

1

2

Small-scale

0.481

High

450522

FAS1
Fatty acid synthase subunit beta

1

3

Small-scale

0.481

High

647699

KPC1
Protein kinase C-like 1

1

6

Small-scale

0.481

High

647718

MCM5
Minichromosome maintenance protein 5

1

7

Small-scale

0.481

High

647732

RAD24
Checkpoint protein RAD24

1

8

Small-scale

0.481

High

647735

MRE11
Double-strand break repair protein MRE11

1

1

Small-scale

0.481

High

647743

UBL1
Ubiquitin carboxyl-terminal hydrolase YUH1

1

2

Small-scale

0.481

High

647744

PP2C1
Protein phosphatase 2C homolog 1

1

3

Small-scale

0.481

High

647763

VPS29
Vacuolar protein sorting-associated protein 29

1

1

Small-scale

0.481

High

647854

AIM7
Protein AIM7

1

2

Small-scale

0.481

High

647861

PMG3
Phosphoglycerate mutase 3

1

3

Small-scale

0.481

High

603008

EST1
Telomere elongation protein EST1

1

0

Small-scale

0.480

High

408287

GAL1
Galactokinase

1

0

High-throughput

0.479

High

419359

H2A1
Histone H2A.1

1

4

Small-scale

0.479

High

448923

TOP2
DNA topoisomerase 2

1

2

Small-scale

0.479

High

561286

DPOLA
DNA polymerase alpha catalytic subunit A

1

2

High-throughput

0.479

High

586426

PCNA
Proliferating cell nuclear antigen

1

3

High-throughput

0.479

High

638779

REB1
DNA-binding protein REB1

1

1

Small-scale

0.479

High

638971

VPS1
Vacuolar protein sorting-associated protein 1

1

3

Small-scale

0.479

High

643602

SNF2
Transcription regulatory protein SNF2

1

9

Small-scale

0.479

High

647008

RPC2
DNA-directed RNA polymerase III subunit RPC2

1

3

High-throughput

0.479

High

647708

CDC48
Cell division control protein 48

1

2

High-throughput

0.479

High

647715

TF2B
Transcription initiation factor IIB

1

2

High-throughput

0.479

High

647716

TF3B
Transcription factor IIIB 70 kDa subunit

1

2

High-throughput

0.479

High

647719

FUN30
ATP-dependent helicase FUN30

1

11

Small-scale

0.479

High

647721

RPC3
DNA-directed RNA polymerase III subunit RPC3

1

1

High-throughput

0.479

High

647725

HIR2
Protein HIR2

1

7

Small-scale

0.479

High

647729

SWI3
SWI/SNF complex subunit SWI3

1

4

Small-scale

0.479

High

647730

STH1
Nuclear protein STH1/NPS1

1

8

Small-scale

0.479

High

647741

GCN1
eIF-2-alpha kinase activator GCN1

1

7

Small-scale

0.479

High

647742

TSA1
Peroxiredoxin TSA1

1

5

Small-scale

0.479

High

647746

TAF14
Transcription initiation factor TFIID subunit 14

1

2

Small-scale

0.479

High

647749

CENPA
Histone H3-like centromeric protein CSE4

1

6

High-throughput

0.479

High

647755

DPOA2
DNA polymerase alpha subunit B

1

1

High-throughput

0.479

High

647756

MCM7
DNA replication licensing factor MCM7

1

6

High-throughput

0.479

High

647757

ISW1
ISWI chromatin-remodeling complex ATPase ISW1

1

11

Small-scale

0.479

High

647762

ECM29
Proteasome component ECM29

1

9

High-throughput

0.479

High

647765

TRA1
SAGA complex/NuA4 acetyltransferase complex subunit TRA1

1

7

Small-scale

0.479

High

647774

IMB4
Importin subunit beta-4

1

7

Small-scale

0.479

High

647777

ATAD2
ATPase histone chaperone YTA7

1

4

High-throughput

0.479

High

647781

ULP2
Ubiquitin-like-specific protease 2

1

2

High-throughput

0.479

High

647792

RSC8
Chromatin structure-remodeling complex protein RSC8

1

2

Small-scale

0.479

High

647802

MSN5
Protein MSN5

1

6

Small-scale

0.479

High

647804

MCM6
DNA replication licensing factor MCM6

1

8

High-throughput

0.479

High

647806

EIF2A
Eukaryotic translation initiation factor 2A

1

2

High-throughput

0.479

High

647807

PESC
Pescadillo homolog

1

1

High-throughput

0.479

High

647812

UBP10
Ubiquitin carboxyl-terminal hydrolase 10

1

8

Small-scale

0.479

High

647814

APJ1
J domain-containing protein APJ1

1

6

High-throughput

0.479

High

647817

ARP4
Actin-related protein 4

1

5

Small-scale

0.479

High

647818

HPC2
Histone promoter control protein 2

1

7

Small-scale

0.479

High

647824

ABF2
ARS-binding factor 2, mitochondrial

1

9

Small-scale

0.479

High

647826

TOM1
E3 ubiquitin-protein ligase TOM1

1

4

High-throughput

0.479

High

647855

SCC1
Sister chromatid cohesion protein 1

1

1

High-throughput

0.479

High

647857

RAD59
DNA repair protein RAD59

1

4

Small-scale

0.479

High

647858

SMC4
Structural maintenance of chromosomes protein 4

1

4

Small-scale

0.479

High

647864

EAF3
Chromatin modification-related protein EAF3

1

2

Small-scale

0.479

High

647866

RUVB2
RuvB-like protein 2

1

3

Small-scale

0.479

High

647867

PSF1
DNA replication complex GINS protein PSF1

1

1

Small-scale

0.479

High

647870

H2AZ
Histone H2A.Z

1

5

Small-scale

0.479

High

647844

CORO
Coronin-like protein

1

5

High-throughput

0.470

High

642167

OPI1
Transcriptional repressor OPI1

1

0

Small-scale

0.460

High

647700

UBP1
Ubiquitin carboxyl-terminal hydrolase 1

1

0

Small-scale

0.460

High

647747

CENPC
Inner kinetochore subunit MIF2

1

0

Small-scale

0.460

High

647759

ECM21
Protein ECM21

1

0

Small-scale

0.460

High

647788

MAF1
Repressor of RNA polymerase III transcription MAF1

1

0

Small-scale

0.460

High

647794

MHP1
MAP-homologous protein 1

1

0

Small-scale

0.460

High

647798

DPOD3
DNA polymerase delta subunit 3

1

0

Small-scale

0.460

High

647799

MOG1
Nuclear import protein MOG1

1

0

Small-scale

0.460

High

647834

APC4
Anaphase-promoting complex subunit 4

1

0

Small-scale

0.460

High

647853

MED2
Mediator of RNA polymerase II transcription subunit 2

1

0

Small-scale

0.460

High

647865

PAA1
Polyamine N-acetyltransferase 1

1

0

Small-scale

0.460

High

647868

LDB19
Protein LDB19

1

0

Small-scale

0.460

High

448284

PDE2
3',5'-cyclic-nucleotide phosphodiesterase 2

1

0

High-throughput

0.458

High

594192

RPB3
DNA-directed RNA polymerase II subunit RPB3

1

0

High-throughput

0.458

High

647698

DPB2
DNA polymerase epsilon subunit B

1

0

Small-scale

0.458

High

647707

SGF29
SAGA complex subunit SGF29

1

0

Small-scale

0.458

High

647733

MG101
Mitochondrial genome maintenance protein MGM101

1

0

Small-scale

0.458

High

647736

RSC7
Chromatin structure-remodeling complex subunit RSC7

1

0

Small-scale

0.458

High

647751

MUD2
Splicing factor MUD2

1

0

Small-scale

0.458

High

647760

YBY7
UPF0303 protein YBR137W

1

0

Small-scale

0.458

High

647779

PSF2
DNA replication complex GINS protein PSF2

1

0

Small-scale

0.458

High

647790

IES1
Ino eighty subunit 1

1

0

Small-scale

0.458

High

647795

SUM1
Suppressor of mar1-1 protein

1

0

Small-scale

0.458

High

647800

BIR1
Chromosomal passenger complex protein BIR1

1

0

Small-scale

0.458

High

647808

RT102
Regulator of Ty1 transposition protein 102

1

0

Small-scale

0.458

High

647809

MSO1
Protein MSO1

1

0

High-throughput

0.458

High

647813

NAF1
H/ACA ribonucleoprotein complex non-core subunit NAF1

1

0

Small-scale

0.458

High

647829

MTQ2
eRF1 methyltransferase catalytic subunit MTQ2

1

0

High-throughput

0.458

High

647839

BUR2
Protein BUR2

1

0

Small-scale

0.458

High

647842

EAF1
Chromatin modification-related protein EAF1

1

0

Small-scale

0.458

High

647843

TFC6
Transcription factor tau 91 kDa subunit

1

0

High-throughput

0.458

High

647845

RSC3
Chromatin structure-remodeling complex protein RSC3

1

0

Small-scale

0.458

High

647852

YL11B
Transposon Ty1-LR1 Gag-Pol polyprotein

1

0

High-throughput

0.458

High

647862

IES3
Ino eighty subunit 3

1

0

Small-scale

0.458

High

647871

CENPX
Inner kinetochore subunit MHF2

1

0

High-throughput

0.458

High

647768

HAL5
Serine/threonine-protein kinase HAL5

1

9

High-throughput

0.457

High

510206

HSP77
Import motor subunit, mitochondrial

1

6

High-throughput

0.445

High

523086

HSP71
Heat shock protein SSA1

1

3

High-throughput

0.445

High

525946

HSP72
Heat shock protein SSA2

1

3

High-throughput

0.445

High

543845

SSB1
Ribosome-associated molecular chaperone SSB1

1

3

High-throughput

0.445

High

547764

NHP6A
Non-histone chromosomal protein 6A

1

8

High-throughput

0.445

High

626590

HSP60
Heat shock protein 60, mitochondrial

1

4

High-throughput

0.445

High

647701

ROX1
Repressor ROX1

1

4

High-throughput

0.445

High

647706

MAS5
Mitochondrial protein import protein MAS5

1

5

High-throughput

0.445

High

647720

HS104
Heat shock protein 104

1

3

High-throughput

0.445

High

647726

ZUO1
Zuotin

1

9

High-throughput

0.445

High

647728

HSP7F
Heat shock protein homolog SSE1

1

7

High-throughput

0.445

High

647738

DOM34
Protein DOM34

1

1

High-throughput

0.445

High

647739

HSP78
Heat shock protein 78, mitochondrial

1

2

High-throughput

0.445

High

647740

MSN2
Zinc finger protein MSN2

1

3

High-throughput

0.445

High

647764

SSZ1
Ribosome-associated complex subunit SSZ1

1

2

High-throughput

0.445

High

647770

CAJ1
Protein CAJ1

1

8

High-throughput

0.445

High

647783

DJP1
DnaJ-like protein 1

1

7

High-throughput

0.445

High

647789

TCPH
T-complex protein 1 subunit eta

1

5

High-throughput

0.445

High

647796

JJJ2
J protein JJJ2

1

6

High-throughput

0.445

High

647838

HSP7Q
Iron-sulfur cluster biogenesis chaperone, mitochondrial

1

7

High-throughput

0.445

High

394025

EFTU
Elongation factor Tu, mitochondrial

1

3

Small-scale

0.439

High

618851

GAP1
General amino-acid permease GAP1

1

2

Small-scale

0.439

High

647750

SRPB
Signal recognition particle receptor subunit beta

1

11

Small-scale

0.439

High

647801

IML1
Vacuolar membrane-associated protein IML1

1

5

Small-scale

0.439

High

647785

LYS14
Lysine biosynthesis regulatory protein LYS14

1

0

High-throughput

0.438

High

647823

RSC4
Chromatin structure-remodeling complex subunit RSC4

1

7

Small-scale

0.437

High

647863

DBP10
ATP-dependent RNA helicase DBP10

1

9

High-throughput

0.437

High

647758

SEA4
SEH-associated protein 4

1

0

High-throughput

0.426

High

647860

SMT3
Ubiquitin-like protein SMT3

1

1

Small-scale

0.408

High

647769

TCPD
T-complex protein 1 subunit delta

1

7

High-throughput

0.407

High

647778

JEM1
DnaJ-like chaperone JEM1

1

5

High-throughput

0.407

High

647780

TCPE
T-complex protein 1 subunit epsilon

1

6

High-throughput

0.407

High

647793

ERJ5
ER-localized J domain-containing protein 5

1

5

High-throughput

0.407

High

647786

MMM1
Maintenance of mitochondrial morphology protein 1

1

0

Small-scale

0.279

Low

647791

IOC3
ISWI one complex protein 3

1

0

High-throughput

0.279

Low

647761

MCFS2
Medium-chain fatty acid ethyl ester synthase/esterase 2

1

0

Small-scale

0.251

Low

647767

AXL2
Axial budding pattern protein 2

1

0

Small-scale

0.251

Low

647787

VMA21
Vacuolar ATPase assembly integral membrane protein VMA21

1

0

Small-scale

0.251

Low

647811

YN9A
Uncharacterized isomerase YNR071C

1

0

Small-scale

0.251

Low

647848

ALR1
Magnesium transporter ALR1

1

0

Small-scale

0.251

Low

647859

YD180
Uncharacterized membrane protein YDL180W

1

0

Small-scale

0.251

Low

518709

ADH4
Alcohol dehydrogenase 4

1

0

Small-scale

0.249

Low

647714

PEX3
Peroxisomal biogenesis factor 3

1

0

High-throughput

0.249

Low

647825

RSC9
Chromatin structure-remodeling complex subunit RSC9

1

0

Small-scale

0.249

Low

647822

UBC7
Ubiquitin-conjugating enzyme E2 7

1

0

High-throughput

0.238

Low


Service provided by Combinatics, Japan

Last updated: 11Aug2025